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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Agrotek UMMat Biosel: Biologi Science and Education Bioscientist : Jurnal Ilmiah Biologi Perbal : Jurnal Pertanian Berkelanjutan
Sembiring, Albert
Department Of Horticultural Agribusiness, Politeknik Wilmar Bisnis Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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ISOLATION OF ANTIFUNGI BACTERIA FROM BANANA RHIZOSPHERE TO INHIBIT Fusarium Oxysproum f.sp cubense (FOC) GROWTH Albert Sembiring; Natalia Lusianingsih Sumanto
JBIO: jurnal biosains (the journal of biosciences) Vol 5, No 3 (2019): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v5i3.15293

Abstract

Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.
ISOLASI BAKTERI PENGHASIL ASAM INDOL ASETAT (AIA) DAN PENGARUHNYA TERHADAP VIABILITAS BENIH CABAI MERAH Albert Sembiring; Natalia Lusianingsih Sumanto
Jurnal Agrotek Ummat Vol 8, No 1 (2021): April 2021
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v8i1.4153

Abstract

Plant growth-promoting rhizobacteria are names that contribute directly or indirectly to plant growth. This group of bacteria will stimulate plant growth through the synthesis of phytohormones (bio-stimulant). Indole acetic acid (IAA) plant endogenous auxin is one of the phytohormone type that these bacteria can generate. IAA has functions for root elongation, cell division and plant growth and development differentiation. This research aims to obtain possible isolates that can generate IAA to optimize the viability of germinating red pepper seed. Bacteria have been isolated by serial dilution spread of Nutrient agar medium was incubation over a 24-hour period at 28oC (room temperature). The amount of IAA was measured by spectrophotometric analysis, selected isolate on the basis of the results, used to encourage the germination of red pepper seed. Ten out of sixteen bacterial isolates could produce IAA in the range of 7.96 ppm - 47.23 ppm. The RC 3 isolate produced the highest IAA while the RC 12 was the other way round. The isolate chosen using this experiment showed an increase in the viability of red pepper seed compare seed germination to 33%, growth spontaneity 37%, vigor index 10%, growth rates 6% and max. growth 13%. IAA isolates could therefore increase the viability of red pepper and got potential of a biofertilizer component.
PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN STEK STEVIA (Stevia rebaudiana B.) Natalia Sumanto; Albert Sembiring
Jurnal Agrotek Ummat Vol 8, No 1 (2021): April 2021
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v8i1.4156

Abstract

Stevia (Stevia rebaudiana R.) is a shrub that is widely used as a natural sweetener. Stevia is a natural sweetener that contains stevioside and rebaudioside A that is 300 times higher, not carcinogenic, and low in calories as a sweet taste than cane sugar. This value is a chance to make stevia sugar as a cane sugar companion. The aim of this study is to measure the effect of growing media on stevia cuttings' growth. This test was undertaken by randomized complete block design (RBD) with 6 treatments and 3 replications in the PoliteknikWBI Practice Laboratory. In order to assess the effect of each treatment. The data obtained were analyzed using analysis of variance at P<5%. The results of the research indicated that the mixed media of humus soil and litter compost (P4) provided the highest values for the observed parameters; the height of the stevia cuttings was 49.50 cm, had 222.76 leaves, the fresh leaf weight was 24.98 g, the total of fresh weight and dry weight was 49. 72 g and 21.70 g, respectively. From the results of this study, it can be concluded that the suitable planting medium for stevia propagation using shoot cuttings is a mixture of humus soil and litter compost with a ratio of 1: 1.
Pengaruh Sistem Pemupukan Tetes Terhadap Hasil Tanaman Tomat (Lycopersicum Esculentum Mill) Di Lahan Kering Ardian Elonard; Albert Sembiring
Perbal : Jurnal Pertanian Berkelanjutan Vol 8, No 1 (2020)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/perbal.v8i1.1509

Abstract

Abstrak Tomat merupakan komoditas strategis yang sangat mempengaruhi perekonomian khususnya Indonesia. Hal ini terlihat pada kebutuhan tomat yang sangat tinggi per tahunnya, bahkan konsumsi untuk setiap tahun mengalami kenaikan hampir 7 %. Permasalahan yang muncul adalah rendahnya produktivitas dari tanaman tomat di Indonesia, sehingga perlu perbaikan untuk meningkatkan produktivitas dengan teknologi pemupukan. Tujuan dari penelitian ini adalah untuk melihat pengaruh sistem pemupukan tetes dengan jenis pupuk dan interval pemupukan yang berbeda terhadap hasil tanaman tomat. Penelitian dilaksanakan dengan menggunakan metode percobaan lapangan yang terdiri atas dua faktor dan tiga ulangan, dirancang dengan menggunakan Rancangan Acak Kelompok (RAK). Faktor pertama adalah interval pemberian pupuk dan faktor kedua adalah jenis pupuk. Faktor pertama terdiri dari 3 aras, yaitu interval 1 minggu (I1), interval 3 minggu (I2) dan Interval 5 minggu (I3). Faktor kedua terdiri 2 aras, yaitu pupuk organik cair (J1) dan pupuk anorganik cair (J2). Pemupukan sistem konvensional digunakan sebagai kontrol untuk pembanding terhadap sistem pemupupan tetes (Drip Fertilization). Analisis menggunakan sidik ragam taraf 5 % dan uji lanjut DMRT (Duncan Multiple Range Test) dengan taraf 5 %. Kemudian untuk membandingkan antara perlakuan dengan kontrol dilakukan uji Kontras Ortogonal . Hasil penelitian menunjukkan sistem pemupukan tetes lebih baik dibanding kan dengan sistem pemupukan konvensional serta memiliki tren meningkatkan pertumbuhan vegetatif dan generatif tanaman tomat di lahan kering. Kata Kunci: Tomat, drip fertilization, interval, jenis pupuk
Isolasi Dan Uji Aktivitas Bakteri Penghasil Selulase Asal Tanah Kandang Sapi Albert Sembiring
BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan Vol 8, No 1 (2019): BIOSEL (Biology Science and Education): Jurnal Penelitian Science dan Pendidikan
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.323 KB) | DOI: 10.33477/bs.v8i1.843

Abstract

Plant biomass from waste agricultural can be transformed to useful like bioethanol and organic fertilizer in industry and agriculture using bacterial cellulase is one alternative to convert cellulose become organic fertilizer. This research had an objective to obtain potential bacterial cellulase isolated from the soil around of cowshed in Institut Pertanian Bogor. Bacteria were isolated from soil had used serial dilution that spread in 1% Carboxy Methyl Cellulose Medium (CMC 1%) and it's incubated at 37oC for 48 hours. Qualitative test for bacteria producing cellulose was carried out by picking the selected colony in the centered of CMC medium with adding congo red 1%. Based on the results were obtained three potential isolates could produce cellulose that were KS 0.1, KS 0.7 and KS 9.1. The highest cellulolytic index is 1.33 that produced by KS 0.1. The test quantitative activity of KS 0.1 using spectrophotometry method showed that the highest of enzyme activity at the first day and lowest eighth day during incubation period. Key Words: bacteria, cellulose, soil around of cowshed
Identification of Potential Isolate Phosphate Solubilizing Bacteria Used 16S rRNA Gene Albert Sembiring; Natalia Lusianingsih Sumanto
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.6218

Abstract

Plants always interact with microorganisms either negatively or positively during their life cycle. The Phosphate Solubilizing Bacteria (PSB) group is one example that has some beneficial effects on the plant. PSB can transform insoluble soil inorganic phosphates into soluble phosphorous so plants can absorb them. This research aimed to test the isolate's ability to solubilize phosphorous and use the 16S rRNA marker gene to identify it. Testing the ability of bacteria to solubilize phosphorous on Pikovskaya's agar medium used a qualitative method by observing the halo zone in the colony around it. Then the bacterial isolate was identified by analyzing the result of sequencing the 16S rRNA gene. The RZ02 bacterial isolate showed the highest performance in solubilizing phosphorous, with a 9.1 mm solubilization index. The gene of 16S rRNA was carried out by using primers 27F and 1492R with an amplicon size of 1500 base pairs. Sequencing analysis and construction of a phylogeny tree were conducted by the Mega X neighbor-joining method and showed 100% similarity with Pseudomonas aeruginosa OIS 481. The solubilization index indicated that the bacteria had a potential candidate to be one of the inoculant components for biofertilizer.
Co-Authors Ardian Elonard Natalia Lusianingsih Sumanto Natalia Sumanto