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Daya Motil dan Keutuhan Membran Plasma Spermatozoa Domba Garut (Ovis aries) Pada Penambahan Kolesterol Dalam Pengencer Semen Tris Kuning Telur -, Herdis
Jurnal Sains dan Teknologi Indonesia Vol. 17 No. 1 (2015)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.793 KB) | DOI: 10.29122/jsti.v17i1.3424

Abstract

The research was carried out to study the effect of egg-yolk consentration and cholesterol suplementationto tris - 1.2% maltosa extender on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature Garut rams. Semen was equilibrated at 5oC for three hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. Percentages of motility and intact plasma membrane (IPM) parameters were evaluated every stage of freezing semen. The results show that there were no interaction between both treatmenst on the quality of garut rams frozen semen. In the cholesterol treatment, percentages of progressive motile sperm and intact plasma membrane in control (50,50% and 57,92%) were significantly different (P<0,05) than cholesterol 1,0 mg /100 ml treatment (37,08% and 49,42%) respectively. In conclusion, addition of 10% egg yolk concentrationto Tris –1.2% maltosa extender was not significantly different than 20% egg yolk concentration. The frozen semen quality of control produced the best quality semen compared toboth addition of 0.5mg/100 ml and 1.0 mg/100 ml cholesterol treatments. Cholesterol addition to egg yolk Tris – 1.2% maltosa causes degradation of frozen semen quality.Penelitian dilakukan untuk melihat pengaruh konsentrasi kuning telur dan penambahan kolesterol pada pengencer semen Tris dengan maltosa 1,2% terhadap kualitas semen beku domba garut. Semen dikoleksi seminggu sekali dengan vagina buatan dari enam domba garut jantan. Semen diekuilibrasi pada suhu 5 oC selama tiga jam kemudian dibekukan dan disimpan di dalam nitrogen cair. Metode thawing dilakukan pada suhu 30oC selama 30 detik. Parameter persentase motilitas dan membran plasma utuh dievaluasi pada setiap tahap pembekuan. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara kedua perlakuan terhadap kualitas semen beku domba garut. Perlakuan kolesterol menunjukan bahwa persentase motilitas dan membran plasma utuk pada perlakuan kontrol (50,50% dan 57,92%) lebih tinggi dan berbeda nyata dibandingkan perlakuan1,0 mg/100ml kolesterol (37,08% dan 49,42%). Penambahan kuning telur 10% pada pengencer Tris dengan maltosa 1,2% menghasilkan kualitas semen tidak berbeda dengan penambahan kuninf telur 20%, Penelitian menyimpulkan bahwa kualitas semen beku terbaik diperoleh pada perlakuan kontrol lebih tinggi dibandingkan perlakuan kolesterol 0,5 mg/100 ml dan 1,0 mg/100 ml. Penambahan kolesterol akan menurunkan kualitas semen beku domba garut.Keywords: cholesterol, motility, membrane plasm, garut ram, spermatozoa.
Peningkatan Kualitas Spermatozoa Epididimis Kerbau Belang yang Dikriopreservasi dengan Beberapa Konsentrasi Sukrosa (THE QUALITY ENHANCEMENT OF EPIDIDYMAL SPERMATOZOA OF SPOTTED BUFFALO CRYOPRESERVING WITH VARIOUS SUCROSE CONCENTRATIONS) Muhammad Rizal; Herdis -; Yulnawati -; Hera Maheshwari
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian dilakukan untuk mengetahui kualitas spermatozoa epididimis kerbau belang selama proses pembekuan (kriopreservasi). Bahan pengencer yang digunakan adalah AndroMed® sebagai kontrol (AM) serta kombinasi AndroMed® dengan 0,2% (S0,2) dan 0,4% sukrosa (S0,4). Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa epididimis kerbau belang setelah thawing dalam bahan pengencer AM (41%), nyata (P<0,05) lebih rendah dibandingkan dengan S0,2 (46%) dan S0,4 (46%). Demikian pula halnya dengan persentase hidup spermatozoa setelah thawing dalam bahan pengencer AM, S0,2, dan S0,4 secara berturut-turut adalah 52,2; 59,8 dan 60,8% (P<0,05). Sementara itu, tidak terdapat perbedaan nyata (P>0,05) pada persentase membran plasma utuh (MPU) setelah thawing dalam ketiga bahan pengencer. Persentase MPU setelah thawing perlakuan AM, S0,2, dan S0,4 adalah masing-masing 68; 68,8 dan 66,8%. Dapat disimpulkan bahwa penambahan sukrosa sebagai krioprotektan eksternal ke dalam bahan pengencer AndroMed® dapat meningkatkan kualitas spermatozoa epididimis kerbau belang setelah thawing.
Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair Yulnawati -; Hera Maheshwari; Muhammad Rizal; Herdis -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to determine the effect of maltose supplement in andromed based semen extender on theviability of buffalo’s epididymal spermatozoa following 12 and 24 hour storage as liquid semen. Epididymalspermatozoa were collected by combined methods of slicing, flushing and tissue pressure. The spermatozoawas then diluted with three different extenders, i.e Andromed only (A) as control, Andromed + 0.2% w/vMaltose (M1) and Andromed + 0.4% w/v Maltose (M2) as treatments. The percentages of progressivelymotil spermatozoa in the liquid semen in the following treatment of A, M1 and M2 were 48.33%; 53.33%and 55% respectively (after 12h of storage), and 45%; 46.67% dan 45% respectively (after 24h of storage).Besides, the percentage of live spermatozoa in A, M1 and M2 after 12h of storage were 70.33%; 72 % and72.33 % and after 24h of storage were 66.33%; 70% and 70.67 %. In conclusion, the addition of 0.2% and0.4% w/v maltose into Andromed extender could maintain the life of the spotted buffalo epididymalspermatozoa after storage for 4°C up to 24 hours.
Co-Authors Anak Agung Gede Sugianthara HERA MAHESHWARI Yulnawati -