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All Journal Hemera Zoa
I. Djuwita .
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Biochemistry, Genetics & Molecular Biology

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Restriction Fragment Length Polymorphism (RFLP) gen sitokrom b dari delapan species burung D.A. Wibowo .; W.E. Prasetyaningtyas .; I. Djuwita .
Hemera Zoa Vol. 1 No. 2 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The aim of this research is to study the Restriction Fragment Length Polyorphism (RFLP) of eight species local birds of the cytochrome b mitochondrial DNA fiagment. The results gainedfiom this study is expected to be used as basic information for tissue birds species recognition. Eight bird I I tissues collected from Bogor area were preserved in 4,5 M NaCI containing 25% DMSO. DNA were isolated using ammonium acetat precipitation. Universal oligonucleotide cytochrome b primers L14841/H15149 were used for amplification of the mitochondrial cytochrome b DNA fragment using Polymerase Chain Reaction (PCR) method, followed by determination of the PCR product by electrophoresis. The amplicon were digested using restriction enzymes Hinf I (2,5 unit) and Rsa I (20 unit) for 2 and 6 hours, respectively. The calculation of the DNA amplicon and restriction fragment is done by measuring the distance between sample DNA migration with the DNA ladder in 2% agarose gel. The results showed that eight local birds species have 359 bp amplicon, and have different Hinf I restriction fragment size, except between scaly-breasted munia and streaked weaver, and also have difflerent Rsa I restriction fragment size except streaked weaver and javan turtle dove, and also chicken and yellow-vented bulbul. In conclusion, universal oligonucleotide cytochrome b primer followed by digestion with Hinf I and Rsa I restriction enzymes can be used for the eight birds species recognition.  
Karakteristik histologi perkembangan folikel ovariumfase luteal pada kancil (Tragulus javanicus) Hamny .; S. Agungpriyono .; I. Djuwita .; W.E. Prasetyaningtyas .; I.Nasution .
Hemera Zoa Vol. 2 No. 1 (2010): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The lesser mouse-deer (Tragulus javanicus) is one of the Indonesia biodiversity. This animal distributes only in South-East Asia and Hindia. The lesser mouse-deer is the smallest ungulate in the world and regarded as an ideal model to biomedic and ruminant research. As their population becoming extincted, conservation efSort is very important. One of the eflorts in supporting the lesser mouse-deer conservation is by improving our knowledge reproductive physiology of the female lesser mouse-deer. The aim of this study was to investigate the histological characteristic of ovarian follicles development of the female lesser mouse-deer. Experiment was done on the ovary (n=4). The ovary was fixed in Bouin solution andprocessed according to standard of histology. The slides were stained in HE. At luteal phase, the developing follicles can be classified into 10 stages. The number of developing follicles in the left ovary were higher then in the right.  
Co-Authors D.A. Wibowo . Hamny Sofyan I.Nasution . S. Agungpriyono . W.E. Prasetyaningtyas .