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Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Fatimah R, Siti; ., Kisrini; Puspita Febrinasari, Ratih
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Background:Steviaâs leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of steviaâs ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (steviaâs ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilkâs test of normality and Friedmann test (Î± = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (Î± = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistarâs blood glucose level which was given steviaâs ethanol extract or honey. Steviaâs ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistarâs blood glucose on diabetic model Conclusions:Steviaâs ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.

Efek Diuresis Ekstrak Propolis pada Tikus Putih Jantan (Rattus Norvegicus) Fadityo, .; Doewes, Muchsin; ., Kisrini
Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Background:Diuretics are medicine that increase the amount ofÂ urine volume. Propolis has 150 compounds that have biological activities, one of the is flavonoid. Flavonoid known to has diuretic effect. Methods:The research is a laboratory experimental analytic study using randomized controlled trial done at Laboratory of Setia Budi University Surakarta in June 2012. The research subject is a number of 35 male white rats of Wistar strain. They were divided into five treatment groups, each group consists of seven rats. Simple random sampling is used for sampling technique. Before the treatment,Â rats were adapted for 7 days and fasted for 48 hours but still provided with drinking water. Propolis extract was given orally. Group I was given aquadest 2.5 ml as negative control, group II was given hydrochlorothiazide 0.3 mg/2.5 ml as positive control, group III was given propolis extract at dose 30 mg/2.5 ml as dose I, group IV was given propolis extract at dose 60 mg/2.5 ml as dose II, and group V was given propolis extract at dose 120 mg/2.5 ml as dose III. Rats urine volume was measured every 6 hours for 24 hours. Results:Result of statistic calculation using Anova test with p < 0.05 shows that there is significant difference in total rats urine volume between group I, II, III, IV, and V. The Post-Hoc test result between group I compared with the others group was significant difference.Group II has the largest urine volume from all groups. Group III has the largest urine volume among the groups that given propolis extract. Group IV has larger urine volume than group V and group V has the least urin volume among the groups that given propolis extract. Conclusions:Through observing amount of urine volume and statistic calculation, it can be concluded that propolis extract has diuretic effect in the male white rats.The increase of propolis extract dose cause the decrease of urine volume. Keywords:Propolis extract, hidroclorotiazid, diuretic

Aktivitas Imunomodulator Ekstrak n-Heksana, Etil Asetat, dan Metanol Biji Jinten Hitam (Nigella sativa L.) Prastiwi, Rini; ., Kisrini; Iqbal, Anwar; Kristi, Aprina
Jurnal Farmasi Sains dan Terapan Vol 2, No 2 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Jinten hitam (Nigella sativa L.) adalah tanaman yang memiliki aktivitas imunomodulator. Kandungan biji jinten hitam adalah minyak atsiri, alkaloid, saponin, tanin, polifenol dan minyak lemak. Penelitian bertujuan untuk mengetahui efek imunomodulator ekstrak biji jinten hitam dengan parameter fagositosis makrofag dan peningkatan imunoglobulin G. Penelitian menggunakan 30 ekor mencit balb/c yang dibagi ke dalam 6 kelompok. Kelompok I diberi ekstrak n-heksana dengan dosis 1,6843 mg/20 gBB, kelompok II diberi eksrak etil astat dosis 0,2682 mg/20 gBB, kelompok III diberi eksrak metanol dosis 0,4272 mg/20 gBB, kelompok IV kontrol positif menggunakan ImboostÂ® dosis 0,065 mg/20 gBB, kelompok V diberi CMC 0,5%, dan kelompok VI kontrol negatif siklofosfamid dosis 2 mg/20gBB. Ekstrak diberikan selama 21 hari, hari ke-7 dan hari ke-14 masing-masing mencit diinjeksikan SRBC (Sheep Red Blood Cells) intraperitoneal. Pada hari ke-21 darah mencit diambil untuk diambil serumnya, kemudian kadar IgG diperiksa dengan ELISA reader. Fagositosis makrofag dilihat berdasarkan aktivitas dan kapasitas makrofag dari sediaan apus cairan intraperitoneal, dengan menghitung persentase 100 makrofag yang melakukan fagositosis. Kapasitas fagositosis ditetapkan berdasarkan jumlah lateks yang difagositosis oleh 50 makrofag aktif. Hasil yang didapatkan ekstrak biji jinten hitam memiliki potensi sebagai imunomodulator dan ekstrak n-heksana memiliki potensi yang tinggi sebagai imunostimulator diban-dingkan ekstrak lain dengan hasil rata-rata presentasi aktivitas fagositosis makrofag 89,5% dan kapasitas fagositosis makrofag 1204 lateks, begitu juga hasil dengan uji IgG. Kata kunci: jinten hitam, imunomodulator, IgG, makrofag.

Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Siti Fatimah R; Kisrini .; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Background:Stevias leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of stevias ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (stevias ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilks test of normality and Friedmann test (? = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (? = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistars blood glucose level which was given stevias ethanol extract or honey. Stevias ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistars blood glucose on diabetic model Conclusions:Stevias ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.

Efek Diuresis Ekstrak Propolis pada Tikus Putih Jantan (Rattus Norvegicus) . Fadityo; Muchsin Doewes; Kisrini .
Nexus Kedokteran Translasional Vol 1, No 2 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Background:Diuretics are medicine that increase the amount of urine volume. Propolis has 150 compounds that have biological activities, one of the is flavonoid. Flavonoid known to has diuretic effect. Methods:The research is a laboratory experimental analytic study using randomized controlled trial done at Laboratory of Setia Budi University Surakarta in June 2012. The research subject is a number of 35 male white rats of Wistar strain. They were divided into five treatment groups, each group consists of seven rats. Simple random sampling is used for sampling technique. Before the treatment, rats were adapted for 7 days and fasted for 48 hours but still provided with drinking water. Propolis extract was given orally. Group I was given aquadest 2.5 ml as negative control, group II was given hydrochlorothiazide 0.3 mg/2.5 ml as positive control, group III was given propolis extract at dose 30 mg/2.5 ml as dose I, group IV was given propolis extract at dose 60 mg/2.5 ml as dose II, and group V was given propolis extract at dose 120 mg/2.5 ml as dose III. Rats urine volume was measured every 6 hours for 24 hours. Results:Result of statistic calculation using Anova test with p < 0.05 shows that there is significant difference in total rats urine volume between group I, II, III, IV, and V. The Post-Hoc test result between group I compared with the others group was significant difference.Group II has the largest urine volume from all groups. Group III has the largest urine volume among the groups that given propolis extract. Group IV has larger urine volume than group V and group V has the least urin volume among the groups that given propolis extract. Conclusions:Through observing amount of urine volume and statistic calculation, it can be concluded that propolis extract has diuretic effect in the male white rats.The increase of propolis extract dose cause the decrease of urine volume. Keywords:Propolis extract, hidroclorotiazid, diuretic

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