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All Journal JURNAL BIOLOGI INDONESIA Proceeding Biology Education Conference
Fifi Afiati
Pusat Penelitian Bioteknologi LIPI Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor 16911 Telp. 021-8754587, Fax. 021-8754588
Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Pengaruh Antioksidan Eksopolisakarida dari Tiga Galur Bakteri Asam Laktat pada Sel Darah Domba Terinduksi tert-Butil Hidroperoksida (t-BHP) Afiati, Fifi; Widad, Nina Ainul; ., Kusmiati
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2196

Abstract

 ABSTRACTExopolysaccharides (EPS) are water soluble polymers synthesized by three strains of lactic acid bacteria (LAB) namely EPS-S1, EPS-S2 and EPS-S7. Some exopolysaccharides (EPSs) produced by lactic acid bacteria (LAB) present potential health-beneficial properties, such as immune stimulation and antioxidative activities. Antioxidants function in several ways including preventing the formation of radicals, scavenging free radicals, formation of hydrogen peroxide and lipid peroxides. The Objectives of this research were to evaluate the antioxidant activities of exopolysaccharides (EPS) extracts from lactic acid bacteria (LAB). The extracts were investigated by using in vitro assays induced tersier-Butilhidroperoksida (t-BHP) 6 µg.ml-1 red blood cell (RBC) for their effects on lipid peroxidation malondealdehyde (MDA) level and  activities of some antioxidant enzymes which includes catalase (CAT) and superoxide dismutase (SOD). Six groups consisted of 3 replicated per group; (I) normal control, RBC (II) negative control, RBC+ t-BHP (III) positive control, RBC+t-BHP+vitamin-C, and (IV-VI) samples, RBC+t-BHP+EPS (S1, S2 and S7) extracts. The results showed that exopolysaccharides (EPS) of three strains of lactic acid bacteria (LAB) increased catalase (CAT) and superoxide dismutase (SOD) enzyme activity and decreased malondealdehyde (MDA) content, exopolysaccharides (EPS)-S2 most potential to increase the activity of catalase (CAT; 77.46%) and superoxide dismutase (SOD; 33.87%) enzyme and inhibit the increase malondealdehyde (MDA; 32.19%) levels. Keywords: exopolysaccharide, lactic acid bacteria, malondealdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) 
PENGARUH ANTIOKSIDAN EKSOPOLISAKARIDA DARI TIGA GALUR BAKTERI ASAM LAKTAT PADA SEL DARAH DOMBA TERINDUKSI TERT-BUTIL HIDROPEROKSIDA (T-BHP) Afiati, Fifi; Widad, Nina Ainul; ., Kusmiati
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2196

Abstract

 ABSTRACTExopolysaccharides (EPS) are water soluble polymers synthesized by three strains of lactic acid bacteria (LAB) namely EPS-S1, EPS-S2 and EPS-S7. Some exopolysaccharides (EPSs) produced by lactic acid bacteria (LAB) present potential health-beneficial properties, such as immune stimulation and antioxidative activities. Antioxidants function in several ways including preventing the formation of radicals, scavenging free radicals, formation of hydrogen peroxide and lipid peroxides. The Objectives of this research were to evaluate the antioxidant activities of exopolysaccharides (EPS) extracts from lactic acid bacteria (LAB). The extracts were investigated by using in vitro assays induced tersier-Butilhidroperoksida (t-BHP) 6 µg.ml-1 red blood cell (RBC) for their effects on lipid peroxidation malondealdehyde (MDA) level and  activities of some antioxidant enzymes which includes catalase (CAT) and superoxide dismutase (SOD). Six groups consisted of 3 replicated per group; (I) normal control, RBC (II) negative control, RBC+ t-BHP (III) positive control, RBC+t-BHP+vitamin-C, and (IV-VI) samples, RBC+t-BHP+EPS (S1, S2 and S7) extracts. The results showed that exopolysaccharides (EPS) of three strains of lactic acid bacteria (LAB) increased catalase (CAT) and superoxide dismutase (SOD) enzyme activity and decreased malondealdehyde (MDA) content, exopolysaccharides (EPS)-S2 most potential to increase the activity of catalase (CAT; 77.46%) and superoxide dismutase (SOD; 33.87%) enzyme and inhibit the increase malondealdehyde (MDA; 32.19%) levels. Keywords: exopolysaccharide, lactic acid bacteria, malondealdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) 
ISOLASI DAN SELEKSI BAKTERI PENGHASIL BIOSURFAKTAN DARI SAMPEL LUMPUR MINYAK, KALIMANTAN TIMUR Sari, Martha; Afiati, Fifi; Kusharyoto, Wien
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Abstract- A total of six bacteria isolated from waste of oil sludge in the area of East Kalimantan were examined to confirm the ability in biosurfactant activity. The active compounds from each bacteria were subjected to screening test of biosurfactant by usingcanola oil as substratein fermentation system. The present study aims to evaluatedthe initial potency ofbiosurfactant producing bacteria from sludge oil in East Kalimantan. All of the isolates were confirmed as producers of biosurfactants with diverse of emulsification activity. Strain SMF4 showed the highest emulsification activity and oil displacement test within 9 days of cultivation.The screening results revealed that the six strains of sludge oil bacteria gave good values in emulsification and oil displacement activity. Keywords: oil sludge, six bacteria, emulsification, and biosurfactant
Co-Authors ., Kusmiati ., Kusmiati Kusharyoto, Wien Martha Sari Widad, Nina Ainul Widad, Nina Ainul