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All Journal Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biodidaktika : Jurnal Biologi dan Pembelajarannya
Agustin Wydia Gunawan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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The Production of Nata Colored by Monascus purpureus J1 Pigments as Functional Food TRESNAWATI PURWADARIA; LISRINA GUNAWAN; AGUSTIN WYDIA GUNAWAN
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1055.427 KB) | DOI: 10.5454/mi.4.1.2

Abstract

Pigments from Monascus sp. may color nata. The mold also produces monacolin K that inhibits HMG-CoA reductase affecting the reduction of blood cholesterol. The aim of this study was to produce the colored nata containing monacolin K. The mold was isolated from commercial angkak. Potato sucrose (PS) and synthetic glucose (SG) media were used to ferment nata with Monascus purpureus J1. Fermentation of nata in PS medium produced red nata, while that in SG medium produced orange nata. The color of nata was similar to the color of supernatant. The optimum red production was obtained after five days of incubation, while the orange production increased until the 14th day. The color concentrations of the supernatant of PS medium containing nata (35.4 μg mL-1) were lower than those without nata (12.4 μg mL-1). The colors of nata looked darker than the color of the supernatant. The concentration of monacolin K in the red nata and the supernatant of PS medium were 0.6 μg mL-1 and 4.6 μg mL-1 respectively, while those in the orange nata and the supernatant of SG medium were 3.2 μg mL-1 and 14.6 μg mL-1 respectively. Dry matter biomass in the PS medium was lower than that in the SG medium. Even though the color of nata looked relatively stable, analyses of the nata water extract that showed a stable condition only occurred in freezing (-20OC) and soaking in buffer solution of pH 12; boiling, water washing, and soaking in a solution of pH 3 and 7 reduced the pigment concentration. Monacolin K concentration was not stable for every treatment, especially for water washing and freezing. Eventhough it was not stable, the boiling nata contained red pigments and monacolin K of 19.7 μg mL-1 and 0.1 μg mL-1 respectively, which can be served as functional food.
Arbuscular Mycorrhizal Fungi in Bisbul Trees (Diospyros blancoi) in Bogor Dwi Retno Ningsih; Kartini Kramadibrata; Agustin Wydia Gunawan
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 20 No. 2 (2013)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.2.381

Abstract

Bisbul (Diospyros blancoi) isa kind of edible fruit and could be beneficial as inhibitor forskin ageing process.Majority of root plants have symbiotic associations with arbuscular mycorrhizal fungi (AMF); however, AMFinbisbul has never been reported. The objective of this study was to observe AMF colonies and to identify AMF spores in bisbul tree rhizospheres and in pot-cultured Pueraria javanica. Roots and soil samples from bisbul rhizospheres were collected from three locations in Bogor area. Roots were stained using trypan blue 0.05%.  Soil samples were air dried, then part of them were used for spore isolation and the rest were used for pot cultures. Spores wereisolated by wet sieving and decanting method and then continued with centrifugation. The results showed that the structures of AMF colonies in bisbul roots were arbuscules, vesicles, coiled hyphae, and  internal hyphae. Isolated AMF spores were identified as Acaulospora longula, A. scrobiculata, A. tuberculata, Claroideoglomus geosporum,Funneliformis etunicatum,Gigaspora candida, G. ramisporophora, Glomus albidum, G. glomerulatum, and Scutellospora calospora. This is the first report of mycorrhizal infection in the root of bisbul and AMF association with bisbul root. Keywords:  Acaulospora, Claroideoglomus, Funneliformis, Gigaspora, Glomus,  Indonesia, Scutellospora
Ragam Cendawan Mikoriza Arbuskula pada Areca di Kebun Raya Bogor Ratu Aqila Fahriny; Agustin Wydia Gunawan
Biodidaktika : Jurnal Biologi dan Pembelajarannya Vol 11, No 1 (2016)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v11i1.2348

Abstract

Areca known as the pinang tree and have benefit as a powerful medicine on men. Arbuscular mycorrhizal fungi (AMF) are symbiotic on almost every root plants, but the diversity of AMF on rizosfer Areca is unknown. Therefore research on variety and colonies AMF on rizosfer Areca is needed. Soil samples of Areca were isolated and processed by wet sieving and decauting method. Root samples of Areca were stained by using trypan blue in lactoglycerol. There were 3 genera of AMF collected from Bogor Botanical Garden (4 species Acaulospora, 3 species Glomus, and 2 species Scutellospora) which is associated with 3 species of Areca. Percentage colonies of AMF rizosfer on roots Areca was counted. Areca has an intermediate type of arbuscular.
Co-Authors Dwi Retno Ningsih Kartini Kramadibrata LISRINA GUNAWAN Ratu Aqila Fahriny Tresnawati Purwadaria