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All Journal Journal of the Indonesian Tropical Animal Agriculture JURNAL BIOLOGI INDONESIA
W. Asmara
Faculty of Veterinary Medicine, Gadjah Mada University, Karangmalang Campus, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Kemampuan Primer General CK4/CK2 Virus Infectious Bronchitis (IBV) untuk Mengaplifikasi Genom IBV Isolat Lapang Indonesia Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
KEMAMPUAN PRIMER GENERAL CK4/CK2 VIRUS INFECTIOUS BRONCHITIS (IBV) UNTUK MENGAPLIFIKASI GENOM IBV ISOLAT LAPANG INDONESIA Dharmayanti, N.L P.I.; Asmara, W.; Artama, W. T.; Indriani, R.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3473

Abstract

ABSTRACTCapability of general primer CK4/CK2 infectious bronchitis virus (IBV) to amplify of genomic IBV field isolates in Indonesia. Avian infectious bronchitis virus (IBV), the prototype of coronaviridae, is highly contangius, economically important pathogen of the chickens. Comparative sequence analysis of IBV S1 gene revealed regions that were conserved among serotypes. Two of conservesd region were used to develop degenarate general primer CK4/CK2 for amplifying IBV genomic RNA by RT-PCR. The aims of this study to demonstrated ability of general primer CK4/CK2 for amplified S1 gene IBV field isolates in Indonesia. The result of this is study suggest that only three IBV field isolates that can be amplified by primer CK4/CK2 that are I-14, I-37 and I-269.Key words: Capability, general primer CK4/CK2, infectious bronchitis virus, field isolates
THE EFFECT OF VIRGIN COCONUT OIL ON LYMPHOCYTE AND CD4 IN CHICKEN VACCINATED AGAINST Avian Influenza VIRUS Yuniwarti, E.Y.W.; Asmara, W.; Artama, W.T.; Tabbu, C.R.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.64-69

Abstract

This research aimed to find preventing alternative of avian influenza (AI) disease in broiler chickenby increasing body immune. Lymphocyte T would directly react to antigen presented to the cell surfaceby antigen presenting cell (APC). Th-CD4 interaction functioned to maintain Th-APC bond intactduring specific antigen activation. Fatty acid in virgin coconut oil (VCO) was potential asimmunostimulant, which therefore could increase chicken immunity through the increase of lymphocyteT and Th-CD4. This research used 40 one-day-old broiler chickens. The method applied was CompletelyRandomized Factorial Design in which the first factor was two levels of vaccine, namely groups of AIvaccinated and unvaccinated. The second factor was four levels of VCO namely 0, 5, 10, 15 mL/kg feed.Day Old Chick (DOC) were divided into eight treatment groups and repeated five times. Feed and waterwere given ad libitum for four weeks. The result showed that the number of lymphocyte and Th-CD4 inchickens given 10 mL per kg feed and vaccinated with AI was higher than that in chickens given VCOwithout AI vaccine.
Polymorphism identification of Diacylglycerol Acyltransferase1 gene and its correlation with fat content of Saanen goat Darmoatmodjo, L. M. Y. D.; Widodo, W.; Asmara, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.211-217

Abstract

Gene markers are often usefull for selection process of cattle on commercial certain phenotypes. Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertion-deletion in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of this study was to identify differences variation in milk fat content among individual Saanen goats, to identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL, 1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%, 3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon 14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene.
Co-Authors C.R. Tabbu Darminto . Darmoatmodjo, L. M. Y. D. Dharmayanti, N.L P.I. Dharmayanti, N.L P.I. E.Y.W. Yuniwarti R. Indriani W. T. Artama, W. T. W. Widodo W.T. Artama