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All Journal Indonesian Journal of Agricultural Science ASIAN JOURNAL FOR POVERTY STUDIES (AJPS)
M.A. Aziz
Bangladesh Tea Research Institute, Moulvibazar, Srimangal 3210, Bangladesh
Agriculture, Biological Sciences & Forestry Social Sciences

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EFFECT OF PERMEABLE VESSEL CLOSURE AND GELLING AGENT ON REDUCTION OF HYPERHYDRICITY IN IN VITRO CULTURE OF CARNATION Winarto, B.; Aziz, M.A.; Rashid, A.A.; Ismail, M.R.
Indonesian Journal of Agricultural Science Vol 5, No 1 (2004): April 2004
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Hyperhydricity, an abnormal morphological appearance and physiologicalfunction, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce  hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and  acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. Thecombination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate upto 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower.
RELEASE OF CLONE BT19 AND BT20 FOR POVERTY REDUCTION IN TEA SECTOR OF BANGLADESH Hossain, M.I.; Ahmed, M.; Aziz, M.A.; Arefin, M.R.; Ashrafuzzaman, M. Ashrafuzzaman; Hossain, M.A.
ASIAN JOURNAL FOR POVERTY STUDIES (AJPS) Vol 3, No 2 (2017)
Publisher : Regional Network on Poverty Eradication

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Bangladesh is a country of 140 million populations where poverty reduction is the prior challenge. With a low level of per capita income, Bangladesh needs to formulate a multi-sectorial strategy for poverty reduction. As Bangladesh is an agricultural based country poverty can be minimized by enhancing agricultural inputs to maximize our production. Tea is an important cash crop in our country. Vast population directly or indirectly depends on our tea industry. The present study was conducted to investigate yield, quality and drought performance of the two newly released variety named BT19 and BT20 by Bangladesh Tea Research Institute. The experiment was conducted at Bangladesh Tea Research Institute experimental farm where four test clones namely Sh/D/11/313, A/8/8, A/17/7, A/22/39 and a well renowned Indian clone TV1 used as a control. The experimental result revealed that amongst the four test clones A/17/7 and A/8/8 are statistically more high yielder and drought tolerant than the control. A/17/7 is more high yielding than A/8/8 but A/8/8 is more drought tolerant. So considering yield, quality and drought potentials, the test clone A/17/7 and A/8/8 have recently released as BT19 and BT20 respectively which can be used as improved planting material in our tea growing areas by small growers and small holders to increase our production, quality as well as job opportunities and national income to eradicate our poverty.
Co-Authors A.A. Rashid B. Winarto M. Ahmed, M. M. Ashrafuzzaman Ashrafuzzaman, M. Ashrafuzzaman M.A. Hossain M.I. Hossain, M.I. M.R. Arefin, M.R. M.R. Ismail