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All Journal Kultivasi Jurnal Agrotek Indonesia OrchidAgro Composite: Jurnal Ilmu Pertanian
Iis Aisyah
Dosen Program Studi Agroteknologi Fakultas Pertanian-Universitas Winaya Mukti, Jl. Raya Bandung-Sumedang Km.29 Tanjungsari 45362, Kab. Sumedang, Jawa Barat, Indonesia.
Agriculture, Biological Sciences & Forestry Veterinary

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Pengaruh air kelapa sebagai sitokinin organik dan sukrosa terhadap pertumbuhan protocorm anggrek (Phalaenopsis hybrid MP 253 x F1 3363 (M)) in vitro Muhammad Isyraq; Lia Amalia; Iis Aisyah
Kultivasi Vol 20, No 1 (2021): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v20i1.31941

Abstract

Abstrak. Sitokinin dan sukrosa dibutuhkan untuk meregenerasi protocorm anggrek. Air kelapa diketahui memiliki kandungan sitokinin sehingga berpotensi dijadikan sebagai sitokinin organik. Percobaan ini bertujuan untuk mempelajari pengaruh konsentrasi air kelapa sebagai sitokinin organik dan sukrosa terhadap pertumbuhan protocorm anggrek. Percobaan ini dilaksanakan di Laboratorium Bioteknologi Tanaman, Fakultas Pertanian Universitas Winaya Mukti, Kecamatan Tanjungsari Kabupaten Sumedang. Percobaan dimulai pada bulan Mei sampai dengan bulan Agustus 2019. Rancangan percobaan yang digunakan adalah rancangan acak kelompok dengan dua faktor dan diulang dua kali. Faktor pertama adalah konsentrasi air kelapa, terdiri dari lima taraf yaitu 0 mL L-1, 50 mL L-1, 100 mL L-1, air kelapa 150 mL L-1, dan air kelapa 200 mL L-. Faktor kedua adalah konsentrasi sukrosa dengan lima taraf, yaitu 0 g L-1, 10 g L-1, 20 g L-1, 30 g L-1 dan 40 g L-1. Hasil percobaan menunjukkan terjadi pengaruh interaksi pada pengamatan jumlah daun umur 12 MST dan bobot segar protocorm. Perlakuan air kelapa 50 mL L-1 dengan tanpa sukrosa menunjukkan jumlah daun yang paling baik dari perlakuan lainnya. Bobot segar protocorm menunjukkan hasil yang terbaik pada umur 12 MST pada konsentrasi air kelapa 100 mL L-1 dengan tanpa sukrosa.Kata kunci: Air Kelapa, In vitro, Protocorm anggrek, Sukrosa. Abstract. Cytokinins and sucrose are needed to regenerate orchid protocorms. Coconut water contains cytokinin so that it has the potential to be used as organic cytokinin. This experiment aims to study the effect of coconut water and sucrose concentrations on the growth of orchid protocorms. This experiment was carried out at the Plant Biotechnology Laboratory, Faculty of Agriculture, University Winaya Mukti Tanjungsari, Sumedang. The experiment was conducted from May to August 2019. The experimental design used a randomized block design with two factors and was repeated twice. The first factor was coconut water concentration, consisted by five levels: 0 mL L-1, 50 mL L-1, 100 mL L-1, 150 mL L-1, and 200 mL L-1. The second factor was sucrose concentration, consisted by five levels: 0 mL L-1, 10 g L-1, 20 g L-1, 30 g L-1 and 40 g L-1. The results of the experiment showed that there were the interaction effect of coconut water and sucrose concentrations on the number of leaves and fresh weight of protocorms. Treatment of 50 mL L-1 coconut water treatment with no sucrose showed the best number of leaves, compared to other treatments. The best fresh weight of protocorm was given by treatment of coconut water 100 mL L-1 without sucrose.Keywords: Coconut water, In vitro, Orchid protocorm, Sucrose.
Keberhasilan Pertumbuhan Stek dan Hasil Bunga Krisan Varietas Puspita Nusantara Akibat Pemberian Dosis Pupuk Kandang Sapi dan Konsentrasi Auksin Lia Amalia; Wahyono Widodo; Anne Berliana; Iis Aisyah; Ai Komariah; Odang Hidayat; Nunung Sondari
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 5 No 2 (2020): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v5i2.4342

Abstract

The availability of nutrients and growth hormones will affect plant growth and yield. Experiments to study the effect of the interaction between cow manure dose and auxin concentration on the success of cuttings growth, growth and yield of Puspita Nusantara chrysanthemum flowers were carried out. The experimental design used was a factorial randomized block design. There are two treatment factors that were repeated 2 times. The first factor is the dose of manure consisting of four levels of 0 t ha-1, 10 t ha-1, 20 t ha-1, and 30 t ha-1. The second factor is the auxin concentration consisting of four levels, namely 0 ml L-1 solution, 1.3 ml L-1 solution, 2.6 ml L-1 solution and 3.9 ml L-1 solution. The results showed that there was an interaction between cow manure dose and auxin concentration on the number of leaves, stem diameter, number of flower buds, flower diameter, flower stalk length, and vaselife. Independently, the dose of cow manure 20 t ha—1, 30 t ha-1 and auxin concentration of 2.6 ml L-1 , 3.9 ml L-1 solution gave higher plant height than the control.
Pengaruh Dosis Kasgot Limbah Hotel Terhadap Pertumbuhan Dan Hasil Tanaman Pakcoy (Brassica rapa L.) Varietas Nauli F1 Rohana Abdullah; Junjun Pratama; Iis Aisyah
OrchidAgro Vol 3, No 2 (2023)
Publisher : Fakultas Pertanian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/orchidagro.v3i2.572

Abstract

This research was conducted in polybags with Andisol soil type in the Experimental Garden of the Faculty of Agriculture, University of Winaya Mukti, Tanjungsari District, Sumedang Regency from June to July 2021. The purpose of this study was to see how the best of hotel food waste BSF frass affected the growth and yield of pakcoy (B. rapa L.). The research utilized a Randomized Block Design with six treatments: A = 0 ton.ha-1 (without BSF frass/control), B= 3 ton.ha-1 cow dung compost, C =1,5 ton.ha-1 BSF frass, D = 3 ton.ha-1 BSF frass, E = 4.5 ton.ha-1 BSF frass, and F = 6 ton.ha-1 BSF frass, all of which are repeated four times.The use of hotel waste BSF frass had a substantial impact on plant height, leaf number, root volume, canopy fresh weight, root fresh weight, canopy dry weight, and root dry weight, according to the findings. Treatment F (6 ton.ha-1) had the best results in terms of plant height, number of leaves, root volume, crown fresh weight, root fresh weight, and canopy dry weight, according to the study's findings. Treatment E (4.5 tons.ha-1) produced the best results in terms of root dry weight.
Pengaruh konsentrasi auksin terhadap pertumbuhan setek pucuk tanaman ciplukan(Physalis peruviana L.) Rizal Nursachya; Endeh Masnenah; Iis Aisyah
Composite: Jurnal Ilmu Pertanian Vol 5 No 2 (2023): Agustus
Publisher : University of Insan Cendekia Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37577/composite.v5i2.580

Abstract

Ciplukan (Physalis peruviana L.) has potential as a medicinal plant. The availability of quality Ciplukan seeds for farmers were still low, while there were also very little information on ciplukan propagation techniques. Therefore it were necessary to study ciplukan propagation techniques. The objectives of research were to study the effect of auxin concentration on the growth of Ciplukan shoot cutting. This experiment have been done at the Waaida Farm in Lembang, Pamulihan village, Sumedang, at an altitude of 943 meters above sea level and temperatures of 18-22 oC, from June to August 2021. The experiment used was a Randomized Block Design (RBD), consisted of five treatments namely A (0 ppm); B (100 ppm); C (200 ppm); D (300ppm); and E (400 ppm) and replicated three times. The result was indicated that the concentration of auxin had an effect on the height of cutting (28 DAP and 42 DAP) and the number of leaves (28 DAP, 42 DAP and 56 DAP) but had no effect on the root volume, fresh weight and dry weight of cutting of Ciplukan. Auxin concentration of 100 ppm (B) had a better effect on the height of cutting (28 DAP and 42 DAP) and number of leaves (28 DAP, 42 DAP and 56 DAP).
Co-Authors Ai Komariah -, Ai Komariah Anne Berliana Endeh Masnenah Hidayat, Odang Junjun Pratama Lia Amalia Muhammad Isyraq Rizal Nursachya Rohana Abdullah Wahyono Widodo