Garuda - Garba Rujukan Digital

Eris Septiana

LIPI

Author-ID : 2386188

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC, AND FLAVONOID CONTENTS OF THE EXTRACT OF ENDOPHYTIC FUNGI DERIVED FROM TURMERIC (Curcuma longa) LEAVES Eris Septiana; Siti Irma Rahmawati; Fauzia Nurul Izzati; Partomuan Simanjuntak
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 16, No 2 (2019)
Publisher : Sanata Dharma University

Air pollution can increase free radicals that may worsen some diseases. Antioxidants such as phenol and flavonoid compounds are known to counteract these free radicals. Long term use of synthetic antioxidants is known to cause bad effects on the body. Therefore, the necessity to search for natural antioxidants from plants and its endophytic microbes continues with the hope to obtain potential natural antioxidants with minimum side effects. The purpose of this study was to determine the antioxidant activity, total phenolic, and flavonoid contents of the extract of endophytic fungi which were derived from the Bogor-originated turmeric leaves via in vitro isolation. DPPH free radical scavenging method was used to determine in vitro antioxidant activity. The total phenolic and flavonoid contents assays were based on the Follin-Ciocalteu and aluminium chloride reagents, respectively. The extract of endophytic fungi of Bo.Ci.Cl.D1 and Bo.Ci.Cl.D2 showed antioxidant activity with IC50 value at 24.04 and 96.08 mg/L, respectively. Total phenolic content of Bo.Ci.Cl.D1 and Bo.Ci.Cl.D2 extracts were 113.47 and 81.83 mg gallic acid equivalent/g extract respectively. Total flavonoid content of Bo.Cl.D1 and Bo.Ci.Cl.D2 extracts were 41.79 and 38.50 mg quercetin equivalent/g extract, respectively. Based on these assays, it could be concluded that the extract of Bo.Ci.Cl.D1 has better antioxidant activities than Bo.Ci.Cl.D2.

AKTIVITAS PENGHAMBATAN BAKTERI PEMBENTUK HISTAMIN DAN ANTIOKSIDAN KAPANG ENDOFIT KUNYIT YANG BERPOTENSI SEBAGAI PENGAWET ALAMI Eris Septiana; Partomuan Simanjuntak
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Microorganisms activity and oxidation process can contribute to food spoilage occurrence. Natural source that have antimicrobial activity and antioxidant are potential as a natural preservative. The aims of this study are to investigate the endophytic fungi from turmeric plant which have antibacterial toward histamine-producing bacteria and antioxidant activity. Antibacterial activity of histamine-producing bacteria was done by paper disc diffusion, whereas the antioxidant activity was performed by free radical scavenging method. In the paper disc diffusion method were obtained 3 isolates which have the highest diameter of inhibition at concentration of 20.000 ppm: from root (15 mm), flower (14 mm) and rhizome (13 mm). Also at the antioxidant assay, the lowest IC50 values were isolates from root (39,52 ppm), flower (157,93 ppm) and rhizome (96 ppm). Endophytic fungi from root, flower and rhizome had antimicrobial and antioxidant activity thus they could potentially used as a natural food preservative.Keywords: antibacterial, antioxidant, endophyte, preservative, turmericABSTRAKAktivitas mikroorganisme dan proses oksidasi dapat menyebabkan kerusakan pangan. Bahan alam yang mempunyai aktivitas sebagai antimikroba dan antioksidan berpotensi sebagai bahan pengawet alami. Penelitian ini bertujuan untuk mengetahui kapang endofit asal tanaman kunyit yang memiliki aktivitas antibakteri penghasil histamin dan antioksidan. Aktivitas antibakteri penghasil histamin dilakukan dengan menggunakan metode difusi cakram kertas, sedangkan aktivitas antioksidan dilakukan dengan metode peredaman radikal bebas. Pada metode difusi cakram kertas didapatkan 3 isolat yang memiliki diameter daya hambat tertinggi sampai konsentrasi 20.000 ppm yaitu berasal dari akar (15 mm), bunga (14 mm) dan rimpang (13 mm). Pada uji antioksidan didapatkan nilai IC50 terendah yaitu isolat yang berasal dari akar (39,52 ppm), bunga (157,93 ppm) dan rimpang (96 ppm). Isolat kapang endofit yang berasal dari akar, bunga dan rimpang memiliki aktivitas antimikroba dan antioksidan sehingga berpotensi digunakan sebagai bahan pengawet makanan alami.Kata kunci: antibakteri, antioksidan, endofit, kunyit, pengawet

AKTIVITAS ANTIDIABETES SECARA IN VITRO EKSTRAK ETANOL DAUN DAN KULIT BATANG BINTANGUR (Calophyllum rigidum Miq.) Eris Septiana; Partomuan Simanjuntak
Jurnal Tumbuhan Obat Indonesia Vol 13 No 2 (2020): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v13i2.1295

ABSTRACT Diabetes mellitus is still a serious health problem both in the world and in Indonesia. The use of plants as a source of antidiabetic medicine is still needed. Bintangur plant, Calophyllum rigidum, contains active compounds that have the potential to act as anti-diabetic drugs but have not been used optimally. The purpose of this study was to determine the in vitro antidiabetic activity of the ethanol extract of Calophyllum rigidum stem bark and leaves. The antidiabetic method used was α-glucosidase enzyme inhibition. Phytochemical screening is based on color change reactions. The results obtained showed that the ethanol extract of stem bark and leaf had α-glucosidase enzyme inhibitory activity with IC50 values of 63.75 and 65.86 µg/mL, respectively. Both extracts contain alkaloids, flavonoids, steroids/triterpenoids, saponins, and tannins, while quinones are only found in the stem bark extracts. The conclusion is that the ethanol extract of the stem bark and leaves of Calophyllum rigidum has active antidiabetic activity through inhibition of the α-glucosidase enzyme. Key words: antidiabetic, α-glucosidase, Calophyllum rigidum, phytochemical screening ABSTRAK Penyakit diabetes mellitus masih merupakan masalah kesehatan serius baik di dunia maupun di Indonesia. Pemanfaatan tanaman sebagai sumber bahan obat antidiabetes masih diperlukan. Tanaman bintangur, Calophyllum rigidum, mengandung senyawa aktif yang berpotensi sebagai obat antidiabetes namun belum dimanfaatkan secara optimal. Tujuan penelitian ini adalah untuk mengetahui aktivitas antidiabetes secara in vitro dari ekstrak etanol kulit batang dan daun Calophyllum rigidum. Metode antidiabetes yang digunakan adalah penghambatan enzim α-glukosidase. Penapisan fitokimia berdasarkan pada reaksi perubahan warna. Hasil yang didapatkan yaitu ekstrak etanol kulit batang dan daun memiliki aktivitas penghambatan enzim α-glukosidase dengan nilai IC50 masing-masing sebesar 63,75 dan 65,86 µg/mL. Kedua ekstrak mengandung golongan senyawa alkaloid, flavonoid, steroid/triterpenoid, saponin, dan tannin, sedangkan kuinon hanya ditemukan pada ekstrak kulit batang. Kesimpulannya ialah bahwa ekstrak etanol kulit batang dan daun Calophyllum rigidum memiliki aktivitas antidiabetes yang aktif melalui penghambatan enzim α-glukosidasi Kata Kunci : antidiabetes, α-glukosidase, Calophyllum rigidum, penapisan fitokimia

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