Data on Riset Kesehatan Dasar (Riskesdas) 2013, 86,1% antibiotics were used and kept in houses without indication and prescription (Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan Rl, 2013). This data supported WHO's study result in 2005 that 50% of the use of drugs in hospitals and health centers was antibiotics. Irrational use of antibiotics were disadvantaged, both, for health, which could induced bacterial resistance, and cost. Unfortunately, people were not yet informed properly. The study, using quantitative methods an interview, assessed the patients' knowledge of antibiotic, described and explored the factors that influence the use of antibiotics and determine the effect of patient education on the use of antibiotics in pharmacies K24Wiyung and Karah Agung Surabaya. The data obtained from the respondents were analyzed with Amos 18. O. From the results, the significant factors that influenced the consumption of antibiotics in pharmacies K24 Wiyung and Karah Agung Surabaya, among others: income (p = 0.031), complaints (p = 0.005), the reason consumption (0.000), resources of information (p = 0001). The factors that were not significant to the use of generic antibiotics include: age (p = 0791), patient knowledge regarding antibiotics (p = 0.404). Thus, information and education about rational use of antibiotics needs to involve these factors in order to be accepted by the patient. To support the rational antibiotic pharmacist should be bold and willing to remind the patient to consult a doctor before taking antibiotics.