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All Journal Folia Medica Indonesiana The Indonesian Biomedical Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology IJRETINA - International Journal of Retina Ophthalmologica Indonesiana
Gatut Suhendro
Department Of Ophthalmology, Faculty Of Medicine, Airlangga University Dr. Soetomo General Hospital, Surabaya, East Java
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health Other

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Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits Luki Indriaswati; Nurwasis; Gatut Suhendro; Soetjipto; Retno Handajani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14785

Abstract

Background: To identify correlation between caspase-3 expression and degeneration of lateral rectus muscleon experimental esotropia in rabbits by using time dependent analysis. Methods: Twenty four rabbits weredivided into 4 groups, first month sham (S1); second month sham (S2); first month esotropia (E1); andsecond month esotropia (E2) model groups. Immunohistochemical and haematoxyllin eosin staining wereperformed. Results: In first month observation no significant difference in caspase-3 expression between S1(1.56 ± 1.03) and E1 (3.93 ± 1.66) groups (p >0.05) and in the degeneration score of lateral rectus musclebetween S1 (1.33 ± 1.36) and E1 (3.83 ± 2.04) groups (p> 0.05). In second month observation no significantdifference in caspase-3 expression between S2 (1.80 ±1.08) and E2 (6.50 ± 3.26) groups (p >0.05); butthere was significant difference in the degeneration score of lateral rectus muscle between S2 (1.16 ± 1.16)and E2 (4.50 ± 1.04) groups (p <0.05). There was a significant correlation between caspase-3 expressionand the degeneration score of lateral rectus muscle (p=0.020; r=0.471). Conclusion: On the experimentalesotropia with prolonged lateral muscle contraction in rabbits, the increasing trend of caspase-3 expressionhas a correlation with the increasing degeneration score of lateral rectus muscle.
Metformin Reduced Collagen Deposition and Contractility, but Increased Collagen Degradation in in vitro Posterior Capsule Opacification Model Frisma Sagara Brilliyanto; Gatut Suhendro; Indri Wahyuni; Maftucah Rochmanti; Windhu Pramono
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1480

Abstract

BACKGROUND: Posterior capsule opacification (PCO) often occurs after cataract surgery. Metformin has been known to have an ability to inhibit fibrosis. This study aimed to investigate the effects of metformin on cell contractility, collagen deposition and degradation in human lens epithelial cells (HLEC) of cataract patients.METHODS: HLEC were isolated from the anterior lens capsule of patients undergoing cataract surgery. The HLEC culture was carried out using explant culture technique. The in vitro PCO model was created by scratching technique on HLEC cultures. The treatment groups were given 0.1, 0.5 and 1 mM metformin, respectively, while the control group were given 10% fetal bovine serum (FBS). On the 7th day after scratching technique, the collagen deposition, collagen degradation and cell contractility were evaluated.RESULTS: Collagen deposition in HLEC was significantly reduced after given 0.1 mM, 0.5 mM and 1 mM metformin (17.92±6.16 mg/mL, 12.92±4.31 mg/mL, 11.25±5.30 mg/mL, respectively), compared to the control group (31.46±7.52 μg/mL, p=0.002). Collagen degradation significantly was increased in the 0.1 mM, 0.5 mM and 1 mM metformin groups (4.77±9.27 mg/mL, 6.59±1.16 mg/mL, 6.35±1.90 mg/mL, respectively) compared to the control group (2.21±2.78 μg/mL, p=0.002). While, collagen contractility in HLEC was significantly reduced in 0.1mM, 0.5mM and 1 mM metformin groups (16.39±3.89%, 13.89±2.59%, 11.93±2.44%, respectively), compared to the control group (44.25±4.95%, p=0.000).CONCLUSION: Metformin reduced collagen deposition and contractility, but increased collagen degradation in HLEC of cataract patients through mechanism of extracellular matrix remodeling.KEYWORDS: metformin, human lens epithelial cell, fibrosis
PENGARUH PENGAWET BEKU (CRYOPRESERVATION) TERHADAP KADAR EPIDERMAL GROWTH FACTOR (EGF) PADA SELAPUT AMNION Ety Retno S; Gunawan Effendi; Gatut Suhendro; I. Handojo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 1 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i1.953

Abstract

to measure the difference of EgF concentration in between fresh amniotic membranes and with cryopreservation in 16 amnioticmembranes. Each amniotic membrane was divided into two parts. the first part was extracted in fresh forms and the second partunderwent cryopreservation with glycerol 50% and was stored at –80° C during 1 month before extraction. Both parts of the membranewere extracted using ultrasonic disintegrator and concentration of EgF was measured from the obtained extract using ELISA method.the average concentration of EgF in the fresh amniotic membrane was 122.76 ± 11.59 pg/g while the average concentration of EgFin the amniotic membrane underwent cryopreservation was 99.34 ± 9.49 pg/g. Average degradation of EgF concentration due tocryopreservation is 18.49% ± 10.20%. EgF concentration in fresh amniotic membrane is significantly higher than the EgF concentrationin amniotic membrane underwent cryopreservation (p = 0.000). Degradation of EgF concentration due to cryopreservation at 95%fidence interval is 12.33% to 24.66%
A First Step to Novel Approach for Treating Alkali Injury of the Cornea: Effect of Platelet Rich Fibrin Lysates on Cultured Rabbit (Oryctolagus cuniculus) Limbal Stem Cell Proliferation Exposed by Sodium Hydroxide Wahyu Endah Prabawati; Gatut Suhendro; Endang Retnowati
Folia Medica Indonesiana Vol. 58 No. 2 (2022): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (964.761 KB) | DOI: 10.20473/fmi.v58i2.36321

Abstract

Highlights: Sodium hydroxide causing platelet rich fibrin stimulates limbal stem cell proliferation in chemical trauma. Limbal stem cell niche and influences limbal stemness was repaired by Platelet Rich Fibrin.   Abstract: Chemical injuries of the eye produce extensive damage to the ocular surface and limbal stem cells, resulting in permanent unilateral or bilateral visual impairment. Alkali injuries occur more frequently than acid injuries. Platelets are a rich source of potential wound healing, promoting polypeptide growth factors. This study aimed to investigate the effect of platelet-rich fibrin (PRF) lysates on limbal stem cell proliferation, which was exposed to sodium hydroxide that resembled limbal stem cell deficiency due to chemical trauma. Confluent rabbit (Oryctolagus cuniculus) limbal stem cells wounded using 20µL of 0.00625 M sodium hydroxide (pH 13) were treated with platelet-rich fibrin lysates (PRF) (0, 5, and 10%). PRF lysates were prepared from peripheral rabbit blood according to Choukroun's method without using anticoagulant and foreign factors for platelet activation. The proliferation of limbal stem cells was measured by a 3-(4,5-dimethylthiazol-2-yl)-2.50 diphenyl tetrazolium bromide (MTT) colorimetric assay at 24, 48, and 72 hours after exposure to sodium hydroxide. Proliferation significantly increased limbal stem cells with PRF lysates 5% (p<0.01) and 10% (p<0.01) group compared with the control (PRF 0%). There was no significant difference between PRF lysates of 5% and 10% (p>0.01). The highest proliferation of limbal stem cells was found in the PRF lysates 5% group after 48 hours (100.24%). PRF stimulated limbal stem cell proliferation in chemical trauma caused by the sodium hydroxide model. PRF repaired the limbal stem cell niche and influenced the limbal stemness. The present findings warrant further research on PRF as a novel alternative treatment for limbal stem cell deficiency.
IMPROVEMENT OF LEUKEMIC RETINOPATHY AFTER LEUKAPHERESIS IN CHRONIC MYELOGENOUS LEUKEMIA WITH LEUKOSTASIS Ruchyta Ranti; Sauli Ari Widjaja; Wimbo Sasono; Muhammad Firmansjah; Ima Yustiarini; Ady Dwi Prakosa; Moestidjab Moestidjab; Gatut Suhendro
International Journal of Retina Vol 3 No 1 (2020): International Journal of Retina (IJRetina) - INAVRS
Publisher : Indonesian Vitreoretinal Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35479/ijretina.2020.vol003.iss001.87

Abstract

ABSTRACT Introduction: to report a case of bilateral leukemic retinopathy due to leukostasis that was successfully managed by leukapheresis. Case Presentation: 31-year-old male with mild visual disturbance was referred to ophthalmology department. He suffered from Chronic Myelogenous Leukemia (CML) with white blood cell (WBC) count 533.900/microL. He was started on hydroxyurea, allopurinol, and once leukapheresis. Ophthalmologic evaluation revealed visual acuity of 4/4 in the right eye and 4/6,3 in the left eye. Funduscopy examination showed the presence of bilateral papilledema, venous engorgement, tortuosity, and retinal hemorrhages. Then this patient continued with second leukapheresis. Result: Visual acuity, laboratory examination, and funduscopic finding was evaluated. His visual acuity was improved, papilledema and retinal blood vessels abnormality had markedly reduced concurring with the patient’s hematological remission. Decreasing WBC count after leukapheresis has improved blood flow that reflected from the retinal findings and visual acuity improvement. Conclusion: Leukapheresis treatment is sufficient to improved clinical condition for leukemic retinopathy caused by CML with leukostasis. Keywords: chronic myelogenous leukemia (CML), hyperleukocytosis, leukostasis, leukemic retinopathy
Effect of Pterygium Size on Astgmatism and Corneal Topography Before and After Surgery Mochammad Sjarifudhin; Gatut Suhendro
Majalah Oftalmologi Indonesia Vol 41 No 2 (2015): Ophthalmologica Indonesiana
Publisher : The Indonesian Ophthalmologists Association (IOA, Perhimpunan Dokter Spesialis Mata Indonesia (Perdami))

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35749/journal.v41i2.31

Abstract

Background: Objective: To analyze the association between pterygium size with astigmatism and corneal topography changes before and after surgery. Methods: This study was conducted at Dr. Soetomo General Hospital and Surabaya Eye Clinic on February-June 2013. In 31 eyes of 20 patients with primary pterygium, size of pterygium was measured in millimeters from the limbus (horizontal length) by caliper and the beam of slit lamp microscope. Twenty four eyes with pterygium type 2 and 3 were excised with bare sclera technique and intraoperative MMC application. Astigmat and corneal topography was investigated with the help of Orbscan preoperatively and 4-6 weeks postoperatively. Result: The size of pterygia had a significant correlation on the elevation of the preoperative corneal astigmatism and irregularities (p<0.001). The mean Sim K astigmatism (p=0.005) and irregularity index (<0.001) significantly decreased after pterygium surgery. The mean refractive power significantly increased after the operation (p<0.001). Conclusion: Pterygium leads to significant changes in corneal refractive status, which increase with the increase in the size of pterygia and improve following pterygium surgery. Keywords: Pterygium, astigmatism, corneal topography
Co-Authors Ady Dwi Prakosa Endang Retnowati Ety Retno S Frisma Sagara Brilliyanto Gunawan Effendi I. Handojo Ima Yustiarini Indri Wahyuni Luki Indriaswati Maftucah Rochmanti Mochammad Sjarifudhin Moestidjab Moestidjab Muhammad Firmansjah Nurwasis Nurwasis Retno Handajani Ruchyta Ranti Sauli Ari Widjaja SOETJIPTO Wahyu Endah Prabawati Wimbo Sasono Windhu Pramono