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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Bioleuser Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Siti Maulida
Program Studi Budidaya Perairan, Fakultas Kelautan Dan Perikanan, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh, Aceh 23111
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering

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Kondisi Biometrik Kerang Darah, Tegillarca granosa, di Pesisir Pantai Utara Kota Banda Aceh Ilham Zulfahmi; Kamaliah Helmi; Siti Rahmah; Neri Kautsari; Siti Maulida; Firman Muhammad Nur
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 4 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.4.620

Abstract

The blood clam, Tegillarca granosa (Linnaeus, 1758) is one of the economically important aquatic organisms. Therefore, information related to biometric conditions is crucial as preventive and responsive efforts to manage blood clams. This study aims to analyze the biometric condition of blood clams collected from the northern coast of Banda Aceh City. Blood-clam samples were collected from three locations, namely Alue Naga, Tibang, and Deah Raya. A total of 300 blood clams were observed. The biometric parameters included the distribution of length and weight classes, the relationship between length and weight, condition factors, ratios, and correlations between total weight, meat weight, and shell weight were measured. The results showed that the majority of blood clams found in Deah Raya were in the smaller length and weight classes (30-32 mm and 12,25-17,24 g) compared to those found in Alue Naga (33-35 mm and 17,25-22,24 g) and Tibang (42-44 mm and 22,25-27,24 g). Despite having identical growth patterns (negative allometric) and condition-factor values, the blood clams collected from Alue Naga had higher meat weight ratios than those collected from the other two locations. The correlation values between the weight of the meat and the total weight of the blood clams collected from Deah Raya tends to be lower than those of blood clams obtained from Alue Naga and Tibang, which are 0,55; 0,81; and 0,78, respectively. Keywords: biometric, environmental factor, Deah Raya
Potensi ekstrak daun flamboyan [Delonix regia (Boj. Ex Hook.) Raf.] terhadap peningkatan aktivitas dan kapasitas makrofag Rosnizar Rosnizar; Siti Maulida; Kartini Eriani; Suwarno Suwarno
Jurnal Bioleuser Vol 1, No 3 (2017): Desember 2017
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.815 KB)

Abstract

Aktivitas dan kapasitas makrofag merupakan faktor penting dalam menjaga sistem imun dalam tubuh. Beberapa senyawa berasal dari tumbuhan seperti daun flamboyan [Delonix regia (Boj. ex hook.) Raf.] dilaporkan banyak mengandung senyawa flavonoid yang berpotensi sebagai imunostimulan dan dilaporkan berpengaruh terhadap peningkatan aktivitas dan kapasitas fagositosis makrofag. Penelitian ini mengkaji tentang potensi  daun flamboyan sebagai agen imunostimulan pada peritonium mencit (Mus muculus) yang diinfeksi dengan bakteri staphylococcus aureus. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari lima perlakuan dan lima ulangan yaitu kontrol negatif (akuades) (P0), kontrol positif (Stimuno) (P1), ekstrak daun flamboyan pada dosis 250 mg/kg bb (P2), 500 mg/kg bb (P3) dan 750 mg/kg bb (P4). Mencit diberikan ekstrak selama 10 hari secara intubasi esofagus dan pada hari ke 11 diinfeksi dengan bakteri Staphylococcus aureus secara intraperitoneal. Jumlah makrofag yang aktif memfagositosis dan jumlah bakteri yang di fagosit menjadi parameter pengukuran. Hasil menunjukkan ekstrak daun flamboyan pada dosis 750 mg/kg bb (P4) memiliki nilai rata-rata aktivitas dan kapasitas fagositosis makrofag yang signifikan lebih tinggi berbanding P0, P1, P2, dan P3 (P 0,05). Dari hasil ini disimpulkan bahwa ekstrak daun flamboyan pada dosis 750 mg/kg bb mampu mempengaruhi dan meningkatkan aktivitas dan kapasitas fagositosis makrofag.
Tinjauan kepustakaan tentang pengembangan kriopreservasi sperma ikan asli Indonesia Siti Maulida; Firman M. Nur; Kartini Eriani; Zainal Abidin Muchlisin
Depik Vol 9, No 2 (2020): August 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.316 KB) | DOI: 10.13170/depik.9.2.16572

Abstract

Sperm cryopreservation is one of the methods in preserving germplasm and avoid the extinction of native fish. The success of this technique requires proper use of protocol because each species needs a different response to certain protocols.  However,  very limited of works have been done in Indonesian fish; Presently, there were only 9 species of freshwater, namely; Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, and Tor soro, and one species marine fish Ephinephelus lanceolatus have been successfully developed the cryopreservation protocols.  The objective of this review paper is to summarized and evaluate the best protocol for sperm cryopreservation of above species  The reviewed aspects are including the type of extender and cryoprotectant, freezing process (storage time and temperature), thawing (thawing time and temperature) and observations (including the percentage of motility, viability, abnormality, fertility, and hatching rate).  Keywords: Extender, Cryoprotectant, Freezing, Thawing ABSTRAKKriopreservasi sperma adalah salah satu cara untuk melestarikan plasma nutfah Indonesia sehingga dapat terhindar dari kepunahan. Keberhasilan kriopreservasi sangat tergantung pada protokol yang digunakan, karena setiap spesies memiliki respon yang berbeda terhadap protokol tertentu. Namun sayangnya sangat sedikit penelitian terkait kriopreservasi sperma ikan-ikan asli Indonesia. Sampai saat ini hanya 9 spesies ikan air tawar (Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, dan Tor soro) dan satu spesies ikan laut Ephinephelus lanceolatus yang telah tersedia protokol kriopreservasi spermanya. Oleh karena itu, penelitian ini mencoba merangkum dan mengevaluasi protokol terbaik dalam proses kriopreservasi masing-masing spesies yang tersebut diatas.  Aspek yang direview adalah jenis extender, krioprotektan, proses freezing (lama penyimpanan sperma dan suhu), thawing (lama thawing dan suhu) dan data hasil pengamatan (meliputi persentase motilitas, viabilitas, abnormalitas, fertilitas dan hatching rate.Kata kunci: Extender, Krioprotektan, Freezing, Thawing 
Tinjauan kepustakaan tentang pengembangan kriopreservasi sperma ikan asli Indonesia Siti Maulida; Firman M. Nur; Kartini Eriani; Zainal Abidin Muchlisin
Depik Vol 9, No 2 (2020): August 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.2.16572

Abstract

Sperm cryopreservation is one of the methods in preserving germplasm and avoid the extinction of native fish. The success of this technique requires proper use of protocol because each species needs a different response to certain protocols.  However,  very limited of works have been done in Indonesian fish; Presently, there were only 9 species of freshwater, namely; Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, and Tor soro, and one species marine fish Ephinephelus lanceolatus have been successfully developed the cryopreservation protocols.  The objective of this review paper is to summarized and evaluate the best protocol for sperm cryopreservation of above species  The reviewed aspects are including the type of extender and cryoprotectant, freezing process (storage time and temperature), thawing (thawing time and temperature) and observations (including the percentage of motility, viability, abnormality, fertility, and hatching rate).  Keywords: Extender, Cryoprotectant, Freezing, Thawing ABSTRAKKriopreservasi sperma adalah salah satu cara untuk melestarikan plasma nutfah Indonesia sehingga dapat terhindar dari kepunahan. Keberhasilan kriopreservasi sangat tergantung pada protokol yang digunakan, karena setiap spesies memiliki respon yang berbeda terhadap protokol tertentu. Namun sayangnya sangat sedikit penelitian terkait kriopreservasi sperma ikan-ikan asli Indonesia. Sampai saat ini hanya 9 spesies ikan air tawar (Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, dan Tor soro) dan satu spesies ikan laut Ephinephelus lanceolatus yang telah tersedia protokol kriopreservasi spermanya. Oleh karena itu, penelitian ini mencoba merangkum dan mengevaluasi protokol terbaik dalam proses kriopreservasi masing-masing spesies yang tersebut diatas.  Aspek yang direview adalah jenis extender, krioprotektan, proses freezing (lama penyimpanan sperma dan suhu), thawing (lama thawing dan suhu) dan data hasil pengamatan (meliputi persentase motilitas, viabilitas, abnormalitas, fertilitas dan hatching rate.Kata kunci: Extender, Krioprotektan, Freezing, Thawing 
Tinjauan kepustakaan tentang pengembangan kriopreservasi sperma ikan asli Indonesia Siti Maulida; Firman M. Nur; Kartini Eriani; Zainal Abidin Muchlisin
Depik Vol 9, No 2 (2020): August 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.2.16572

Abstract

Sperm cryopreservation is one of the methods in preserving germplasm and avoid the extinction of native fish. The success of this technique requires proper use of protocol because each species needs a different response to certain protocols.  However,  very limited of works have been done in Indonesian fish; Presently, there were only 9 species of freshwater, namely; Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, and Tor soro, and one species marine fish Ephinephelus lanceolatus have been successfully developed the cryopreservation protocols.  The objective of this review paper is to summarized and evaluate the best protocol for sperm cryopreservation of above species  The reviewed aspects are including the type of extender and cryoprotectant, freezing process (storage time and temperature), thawing (thawing time and temperature) and observations (including the percentage of motility, viability, abnormality, fertility, and hatching rate).  Keywords: Extender, Cryoprotectant, Freezing, Thawing ABSTRAKKriopreservasi sperma adalah salah satu cara untuk melestarikan plasma nutfah Indonesia sehingga dapat terhindar dari kepunahan. Keberhasilan kriopreservasi sangat tergantung pada protokol yang digunakan, karena setiap spesies memiliki respon yang berbeda terhadap protokol tertentu. Namun sayangnya sangat sedikit penelitian terkait kriopreservasi sperma ikan-ikan asli Indonesia. Sampai saat ini hanya 9 spesies ikan air tawar (Channa striata, Chromobotia macracanthus, Barbonymus gonionotus, Mystus nemurus, Osphronemus goramy, Osteochilus hasseltii atau Osteochilus vittatus, Poropontius tawarensis, Rasbora tawarensis, dan Tor soro) dan satu spesies ikan laut Ephinephelus lanceolatus yang telah tersedia protokol kriopreservasi spermanya. Oleh karena itu, penelitian ini mencoba merangkum dan mengevaluasi protokol terbaik dalam proses kriopreservasi masing-masing spesies yang tersebut diatas.  Aspek yang direview adalah jenis extender, krioprotektan, proses freezing (lama penyimpanan sperma dan suhu), thawing (lama thawing dan suhu) dan data hasil pengamatan (meliputi persentase motilitas, viabilitas, abnormalitas, fertilitas dan hatching rate.Kata kunci: Extender, Krioprotektan, Freezing, Thawing 
Co-Authors Firman M. Nur Firman Muhammad Nur Ilham Zulfahmi Kamaliah Kamaliah Kartini Eriani Kartini Eriani Neri Kautsari Rosnizar Rosnizar Siti Rahmah Suwarno Suwarno Zainal Abidin Muchlisin