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All Journal HAYATI Journal of Biosciences
Noor Cholies Zaini
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Potency of Dextranase from Arthrobacter sp. Strain B7 as Dental Plaque Removal AFAF BAKTIR; NOOR CHOLIES ZAINI; UNTUNG MURDIYATMO; KUNTAMAN KUNTAMAN
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.646 KB) | DOI: 10.4308/hjb.12.4.162

Abstract

Dextranase of Arthrobacter sp. strain B7 (B7DEX enzyme) was characterized in this study. This enzyme hydrolyzed sucrose and dextran, but not other glucans (starch, nigeran, cellulose, -soluble glucan). It also hydrolyzed glucan from dental plaque with the activity of 7.38 +/- 66 U/ml, where the activity toward dextran was 31.88 +/- 1.24 U/ml. The enzyme exhibited the pH optimum of 7 and the temperature optimum of 50 0C. Its optimum stability was at pH 7 and 50 0C. The enzyme was inhibited by Fe3+, Cu2+, Zn2+, and Ag+, but not by the anionic detergent (SDS) and the nonionic detergent (Triton-X). The enzyme was activated by Ca2+, Na+, Mg2+, and saliva.
Co-Authors AFAF BAKTIR KUNTAMAN KUNTAMAN UNTUNG MURDIYATMO