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Growth, biomass, and chlorophyll-a and carotenoid content of Nannochloropsis sp. strain BJ17 under different light intensities Muhammad Fakhri; Nasrullah Bai Arifin; Anik Martina Hariati; Ating Yuniarti
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3159.068 KB) | DOI: 10.19027/jai.16.1.15-21

Abstract

ABSTRACT  Nannochloropsis sp. has been identified as sources of live feed and pigment in aquaculture. To increase the production, the optimal environmental conditions for microalgae are required. Light intensity is one of the important factors that significantly affects the biomass and pigment of microalgae. The study aimed to determine the effect of light intensity (1,500; 3,000; and 4,500 lux) on growth, biomass production, chlorophyll-a, and carotenoid content of Nannochloropsis sp. strain BJ17. The results showed that different light intensities significantly affected the growth, biomass, chlorophyll-a and carotenoid contents of Nannochloropsis sp. strain BJ17. Increasing light intensity resulted in the increase of the growth rate, biomass, chlorophyll-a, and carotenoid contents of Nannochloropsis sp. strain BJ17. The cell achieved the highest specific growth rate of 1.729 %/day and the cell concentration of 43.333×106 cell/mL at a light intensity of 4,500 lux. The highest chlorophyll-a and carotenoid concentrations of algae were obtained at 4,500 lux (8.304 μg/mL and 3.892 μg/mL, respectively). This study suggested that increasing light intensity led to the increase in the growth, biomass, chlorophyll-a, and carotenoid content of Nannochloropsis sp. strain BJ17. Keywords: carotenoid, chlorophyll, biomass, growth rate, light intensity  ABSTRAK  Nannochloropsis sp. diketahui sebagai sumber pakan alami dan pigmen pada budidaya perikanan. Budidaya pada kondisi lingkungan yang optimal diperlukan untuk meningkatkan produksi mikroalga. Intensitas cahaya merupakan salah satu faktor esensial yang secara signifikan mempengaruhi biomassa dan pigmen mikroalga. Tujuan penelitian ini adalah untuk menentukan pengaruh intensitas cahaya yang berbeda (1.500, 3.000, and 4.500 lux) terhadap pertumbuhan, produksi biomassa, klorofil-a, dan karotenoid Nannochloropsis sp. strain BJ17. Hasil menunjukkan bahwa intensitas cahaya yang berbeda berpengaruh secara signifikan terhadap pertumbuhan, biomassa dan klorofil-a dan karotenoid Nannochloropsis sp. strain BJ17. Semakin tinggi intensitas cahaya maka laju pertumbuhan, biomassa, kandungan klorofil-a dan total karotenoid Nannochloropsis sp. strain BJ17 semakin tinggi. Laju pertumbuhan spesifik tertinggi 1,729%/hari dan konsentrasi sel maksimum tertinggi 43,333×106 sel/mL dihasilkan pada intensitas cahaya 4.500 lux. Konsentrasi klorofil-a (8,304 μg/mL) dan karotenoid (3,892 μg/mL) tertinggi juga diperoleh pada intensitas cahaya 4.500 lux. Studi ini menunjukkan bahwa peningkatan intensitas cahaya berperan dalam meningkatkan pertumbuhan, produksi biomassa, klorofil-a, dan karotenoid Nannochloropsis sp. strain BJ17. Kata kunci: karotenoid, klorofil, biomassa, pertumbuhan, intensitas cahaya
THE DEVELOPMENT OF ALTERNATIVE FEED AND PROBIOTIC APPLICATION ON CATFISH INSTRUMENTS IN JEMIRAHAN VILLAGES nasrullah bai arifin
Journal of Innovation and Applied Technology Vol 3, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (899.082 KB) | DOI: 10.21776/ub.jiat.2017.003.01.6

Abstract

African catfish (Clarias gariepinus) is widely cultured freshwater species in Indonesia as well as in Jemirahan, Sidoarjo. Two major problems of catfish culture in this area is low survival rate and low margin due to high cost in feed utilization. The purpose of this activity was to evaluate the application of probiotic and alternative feed in catfish culture. Fish fry of 7-9 cm in size was cultivated for 90 days in rearing activity. All tanks were treated by probiotic during one cycle of culture. Probiotic was directly given to the water and administered by feed. The results showed that survival rates obtained during cultured periods was 92%. Meanwhile, feed conversion ratio value was 1.5. It can be concluded that probiotic application was able to improve the survival rate and the use of alternative feed was able to replace commercial feed.
Efikasi Oxytetracycline Terhadap Kesehatan Ikan Lele yang Diinfeksi Bakteri Edwardsiella tarda Qurrota A'yunin; Hartati Kartikaningsih; Sri Andayani; Nasrullah Bai Arifin
JFMR (Journal of Fisheries and Marine Research) Vol 3, No 1 (2019): JFMR VOL 3 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.183 KB) | DOI: 10.21776/ub.jfmr.2019.003.01.15

Abstract

Edwardsielliosis is a disease that commonly infects freshwater fish including catfish (Clarias sp). Edwardsielliosis is caused by Edwardsiella tarda infection and causes mass mortality in catfish farming. Overcoming diseases in freshwater fish can use drugs or antibiotics that are classified as safe and their use is in accordance with government regulations. This study aims to determine the appropriate dosage of Oxytetracycline in catfish infected by E. tarda bacteria through histological analysis. The study design used Completely Randomized Design with 3 treatments, 1 control and 3 replications in each treatment. Catfish used were ± 25 cm in size and the dose of oxytetracycline used were 15, 30, and 45 ppm. The results showed tissue damage edema, vacuolization, necrosis, and haemorhage in fish infected with Edwardsiella tarda bacteria with the lowest damage at 45 ppm of oxytetracycline treatment. The results indicate that oxytetracycline has an effect on muscle histology and recovery of fish health.
Efikasi Oxytetracycline Terhadap Kesehatan Ikan Lele yang Diinfeksi Bakteri Edwardsiella tarda Qurrota A'yunin; Hartati Kartikaningsih; Sri Andayani; Nasrullah Bai Arifin
JFMR (Journal of Fisheries and Marine Research) Vol. 3 No. 1 (2019): JFMR
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.01.15

Abstract

Edwardsielliosis is a disease that commonly infects freshwater fish including catfish (Clarias sp). Edwardsielliosis is caused by Edwardsiella tarda infection and causes mass mortality in catfish farming. Overcoming diseases in freshwater fish can use drugs or antibiotics that are classified as safe and their use is in accordance with government regulations. This study aims to determine the appropriate dosage of Oxytetracycline in catfish infected by E. tarda bacteria through histological analysis. The study design used Completely Randomized Design with 3 treatments, 1 control and 3 replications in each treatment. Catfish used were ± 25 cm in size and the dose of oxytetracycline used were 15, 30, and 45 ppm. The results showed tissue damage edema, vacuolization, necrosis, and haemorhage in fish infected with Edwardsiella tarda bacteria with the lowest damage at 45 ppm of oxytetracycline treatment. The results indicate that oxytetracycline has an effect on muscle histology and recovery of fish health.