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Isolasi Bakteri Termofilik Penghasil Biosurfaktan yang Ber-potensi sebagai Agen MEOR (Microbial Enhanched Oil Re-covery) dari Sumur Minyak di Sungai Angit Rora Eva Laini; A. Napoleon A. Napoleon; Munawar Munawar
Jurnal Penelitian Sains Vol 17, No 1 (2014)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.269 KB) | DOI: 10.56064/jps.v17i1.42

Abstract

Salah satu teknik EOR yang dikembangkan saat ini adalah memanfaatkan mikroba yang dikenal den-gan microbial enhanced oil recovery (MEOR). MEOR merupakan teknologi yang memanfaatkan aktivitas mikro-ba dan dilakukan dengan tujuan meningkatkan perolehan minyak pada suatu reservoir minyak bumi dengan cara menginjeksikan mikroba ke dalam reservoir tersebut. Riset ini bertujuan untuk mendisolasi bakteri termo-filik penghasil biosurfaktan yang berpotensi sebagai agen MEOR. Sampel minyak diambil dari tiga sumur mi-nyak yang berbeda di desa Sungai Angit, Musi Banyuasin menggunakan metode Multiple Sampling. Bakteri yang berhasil diisolasi diuji kemampuannya bertahan hidup pada suhu tinggi dan kemampuannya menghasil-kan biosurfaktan. Ada 8 bakteri hasil isolasi, namun hanya 7 yang lolos uji termofilik dan 3 bakteri yang secara signifikan menghasilkan biosurfaktan, yaitu : Pseudomonas citronellolis, Pseudomonas fluorescens dan Burkholde-ria glumae.
Eksplorasi Bakteri Hidrokarbonoklastik dari Rhizosfer di Lahan Tambang Minyak Rakyat, Kecamatan Babat Toman, Sumatera Selatan Dwi Hardestyariki; Bambang Yudono; Munawar Munawar
Jurnal Penelitian Sains Vol 16, No 3 (2013)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.983 KB) | DOI: 10.56064/jps.v16i3.65

Abstract

This study aims to obtain rhizosphere bacteria as a potential agent of bioremediation. The method of sampling the plant rhizosphere by purposive sampling. Soil samples were taken from around the rhizos-phere of plants that live in soil contaminated oil. Bacteria were isolated from the rhizosphere soil samples, then they were selected into steps : performed purification, selection I and selection II. The among bacteria are tested the synergism, then characterized and identified it’s genus. The results showed that there were as many as 34 rhizosphere bacterial isolates were collected from three different sampling locations. Identification of bacteria that have potential as a bioremediation agent. Each bacterial isolate was identified as Sporosarcina sp. A.4.10, Proteus sp. C.6.7, Actinobacillus sp. A.6.3, and Flavobacterium sp. A.5.8.
Co-Authors A. Napoleon Bambang Yudono Dwi Hardestyariki, Dwi Rora Eva Laini