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Chemical Properties of 15-year-old Teak (Tectona grandis L.f) from Different Seed Sources Ganis Lukmandaru; Pormando Manalu; Tomy Listyanto; Denny Irawati; Rini Pujiarti; Fanny Hidayati; Dian Rodiana
Wood Research Journal Vol 7, No 1 (2016): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2016.7.1.6-12

Fifteen year-old teak wood samples planted in Ciamis FMU (Perhutani Enterprise) were evaluated for their chemical properties. Three seed sources such as conventional seed, clone, and superior wood and radial positions namely sapwood, outer heartwood, and inner heartwood were the observed factors. The specimens were taken from the bottom parts of their sources. Completely randomized design was used. Cell wall components were analyzed by various gravimetric methods.Analysis of variance and Duncan’s test were performed for data analysis. The results showed that no significant difference in the quantity of cell wall components (cellulose, hemicellulose, and lignin), extractives (ethanol-toluene and hot-water solubles), ash, and silica content among the seed sources. Superior teakwood or Jati Plus Perhutani, which has the highest growth rate (2.1~3.6 cm/year) among others, showed a comparative higher average pH values (7.08~7.38) and solubility in 1% NaOH (17.22~17.83%) than other sources. Radial factors significantly affected ethanol-toluene extractive and lignin content. The ethanol-toluene extractive had the highest content (9.30~11.54%) at the outer part of heartwood while lignin content was the lowest (28.12~30.10%) in the inner part. The result indicated some good characteristics of young teak trees compared to the mature ones in relation to wood processing.

Phenol Contents and Antioxidant Activity of Sonokeling (Dalbergia latifolia Roxb) Wood Masendra Masendra; Denny Irawati; Alamratush Shoolichah Ridlo; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.27-34

Dalbergia latifolia or sonokeling is a native species of Java, Indonesia, used as an important wood for furniture and building materials, due to the high of durability and beautiful color. This study, therefore, aim to investigate the phenol composition, represented by total phenolic, flavonoid, and flavanol content, as well as antioxidant activity, conducted by DPPH (1, 1-diphenyl-2-picrylhydrazyl) method on Dalbergia latifolia wood. The sample was extracted using ethanol-toluene solvent in a Soxhlet apparatus, and subsequently subjected to column chromatography. This treatment yielded 12 fractions, which were then evaluated for phenol contents and antioxidant activity. The results showed a high antioxidant activity and total phenolic content in Fr.1- Fr.3, while latifolin was detected and characterized by GC-MS and a literature comparison. Therefore, it was established that the antioxidant activity of D. latifolia wood extractives properly correlated with the total phenolic, but not with the total flavonoid and flavanol contents.

Mycelia Growth of Shiitake (Lentinula edodes) on 4-Wood Species from Leguminaceae Family Dahayu Ratnanindha; Johanes Pramana Gentur Sutapa; Denny Irawati
Wood Research Journal Vol 10, No 1 (2019): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2019.10.1.26-31

Cultivation of Shiitake mushroom (Lentinula edodes) in Indonesia has not been developed yet, due to its low productivity. It happens because of the limited information of the good ways on cultivation Shiitake in Indonesia. One of the factors that affect the growth of mushroom is the media. In Indonesia, the information about the media composition by using various species of wood have not been found yet. Therefore, this research was conducted to determine the effect of using different wood species to the growth of Shiitake. This study used four wood species from the leguminaceae family, those were: Gamal (Glicidia sepium); Johar (Samanea saman); Leucaena (Leucaena leucocephala); and Sengon (Falcataria moluccana). Those were used as the media by adding 12.5% (w/w) of rice bran and 6% (w/w) of CaCO3, and adjusting the moisture content by adding the distillate water to 65, 70, and 75%. Then, the media was put in to the petridisk (ø 90 mm) and inoculated with Shiitake mushroom. During the mycelia growth, the length of mycelia was measured every 2 days until fifty days, and then glucosamine content was analyzed. In addition, chemical analysis was also conducted to each media. The results showed that different wood species resulted different chemical content of media, except the acid soluble lignin content. The growth rate of mycelia was affected by the moisture content of media, meanwhile the glucosamin content is influenced by the moisture content and various species of wood. The best combination to cultivate the Shiitake mushroom was by using media made of gamal with 70% moisture content. There were positive correlation among the ethanol-toluen extracts content and the mycelia growth, however there was negative correlation among the hemicelullose content the mycelia growth.

Chemical Properties of 15-year-old Teak (Tectona grandis L.f) from Different Seed Sources Ganis Lukmandaru; Pormando Manalu; Tomy Listyanto; Denny Irawati; Rini Pujiarti; Fanny Hidayati; Dian Rodiana
Wood Research Journal Vol 7, No 1 (2016): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2016.7.1.6-12

Fifteen year-old teak wood samples planted in Ciamis FMU (Perhutani Enterprise) were evaluated for their chemical properties. Three seed sources such as conventional seed, clone, and superior wood and radial positions namely sapwood, outer heartwood, and inner heartwood were the observed factors. The specimens were taken from the bottom parts of their sources. Completely randomized design was used. Cell wall components were analyzed by various gravimetric methods.Analysis of variance and Duncan’s test were performed for data analysis. The results showed that no significant difference in the quantity of cell wall components (cellulose, hemicellulose, and lignin), extractives (ethanol-toluene and hot-water solubles), ash, and silica content among the seed sources. Superior teakwood or Jati Plus Perhutani, which has the highest growth rate (2.1~3.6 cm/year) among others, showed a comparative higher average pH values (7.08~7.38) and solubility in 1% NaOH (17.22~17.83%) than other sources. Radial factors significantly affected ethanol-toluene extractive and lignin content. The ethanol-toluene extractive had the highest content (9.30~11.54%) at the outer part of heartwood while lignin content was the lowest (28.12~30.10%) in the inner part. The result indicated some good characteristics of young teak trees compared to the mature ones in relation to wood processing.

Phenol Contents and Antioxidant Activity of Sonokeling (Dalbergia latifolia Roxb) Wood Masendra Masendra; Denny Irawati; Alamratush Shoolichah Ridlo; Ganis Lukmandaru
Wood Research Journal Vol 11, No 1 (2020): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2020.11.1.27-34

Dalbergia latifolia or sonokeling is a native species of Java, Indonesia, used as an important wood for furniture and building materials, due to the high of durability and beautiful color. This study, therefore, aim to investigate the phenol composition, represented by total phenolic, flavonoid, and flavanol content, as well as antioxidant activity, conducted by DPPH (1, 1-diphenyl-2-picrylhydrazyl) method on Dalbergia latifolia wood. The sample was extracted using ethanol-toluene solvent in a Soxhlet apparatus, and subsequently subjected to column chromatography. This treatment yielded 12 fractions, which were then evaluated for phenol contents and antioxidant activity. The results showed a high antioxidant activity and total phenolic content in Fr.1- Fr.3, while latifolin was detected and characterized by GC-MS and a literature comparison. Therefore, it was established that the antioxidant activity of D. latifolia wood extractives properly correlated with the total phenolic, but not with the total flavonoid and flavanol contents.

Mycelia Growth of Shiitake (Lentinula edodes) on 4-Wood Species from Leguminaceae Family Dahayu Ratnanindha; Johanes Pramana Gentur Sutapa; Denny Irawati
Wood Research Journal Vol 10, No 1 (2019): Wood Research Journal
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51850/wrj.2019.10.1.26-31

Cultivation of Shiitake mushroom (Lentinula edodes) in Indonesia has not been developed yet, due to its low productivity. It happens because of the limited information of the good ways on cultivation Shiitake in Indonesia. One of the factors that affect the growth of mushroom is the media. In Indonesia, the information about the media composition by using various species of wood have not been found yet. Therefore, this research was conducted to determine the effect of using different wood species to the growth of Shiitake. This study used four wood species from the leguminaceae family, those were: Gamal (Glicidia sepium); Johar (Samanea saman); Leucaena (Leucaena leucocephala); and Sengon (Falcataria moluccana). Those were used as the media by adding 12.5% (w/w) of rice bran and 6% (w/w) of CaCO3, and adjusting the moisture content by adding the distillate water to 65, 70, and 75%. Then, the media was put in to the petridisk (ø 90 mm) and inoculated with Shiitake mushroom. During the mycelia growth, the length of mycelia was measured every 2 days until fifty days, and then glucosamine content was analyzed. In addition, chemical analysis was also conducted to each media. The results showed that different wood species resulted different chemical content of media, except the acid soluble lignin content. The growth rate of mycelia was affected by the moisture content of media, meanwhile the glucosamin content is influenced by the moisture content and various species of wood. The best combination to cultivate the Shiitake mushroom was by using media made of gamal with 70% moisture content. There were positive correlation among the ethanol-toluen extracts content and the mycelia growth, however there was negative correlation among the hemicelullose content the mycelia growth.

Pemanfaatan Limbah Biomassa Sawit Ramah Lingkungan Denny Irawati; Ganis Lukmandaru; Joko Sulistyo; Sigit Sunarta; Tomy Listyanto; Jaka Widada; Nunuk Supriyatno; Yose Rizal
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 6, No 4 (2020): Desember
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Dalam rangka memenuhi kebutuhan lahan untuk pembangunan perumahan karyawan, PT Semen Baturaja Tbk. (PTSB) membuka kurang lebih 27 hektar lahan yang di dalamnya diperkirakan terdapat kurang lebih 2700 batang tanaman kelapa sawit yang kurang produktif. Pembukaan lahan tersebut akan menghasilkan biomassa sawit yang cukup besar. Salah satu usaha pemanfaatan limbah biomassa sawit adalah dengan mengolahnya menjadikompos. Kompos dapat digunakan untuk rehabilitasi lahan bekas tambang oleh PTSB. Hingga saat ini, PTSB belum memiliki pengalaman di bidang pembuatan kompos. Oleh karena itu, PTSB bekerja sama dengan Fakultas Kehutanan, Universitas Gadjah Mada untuk memproduksi pupuk kompos dari limbah biomassa tanaman sawit. Metode yang digunakan pada kegiatan pengabdian ini adalah sosialisasi, pembuatan kompos secara partisipatif, dan pendampingan di lapangan. Luaran dari kegiatan ini berupa kompos limbah biomassa sawit serta pengetahuan mengenai teknologi proses pembuatan kompos bagi pihak PTSB.Jumlah pupuk kompos yang bisa diperoleh dari delapan gundukan daun kelapa sawit adalah 248,9 ton dan dari batang kelapa sawit sebanyak 1.236,6 ton. Pupuk kompos setelah pengomposan selama dua bulan mempunyai rasio C/N 13,7. Biaya yang dikeluarkan untuk pengomposan adalah Rp591.405.000,00 dengan potensi keuntungan sebesar Rp6.093.232.500,00. Pengetahuan perihal pembuatan kompos berdampak pada penghematan pengeluaran PTSB untuk membeli pupuk guna rehabilitasi lahan serta penanganan permasalahan limbah biomassa.

Pemanfaatan Serbuk Kayu untuk Produksi Etanol dengan Perlakuan Pendahuluan Delignifikasi Menggunakan Jamur Phanerochaete Chrysosporium Denny Irawati; Norman Razief Azwar; Wasrin Syafii; I Made Artika
Jurnal Ilmu Kehutanan Vol 3, No 1 (2009)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Utilization of Sawdust to Produce Ethanol Using Delignification Pre-treatment with White Rot Fungi Phanerochaete chrysosporiumCurrently, Indonesia is in the middle ofpetroleum crisis. One ofthe alternative fuels which can be used as a petroleum substitute is ethanol. Ethanol can be produced from timber waste (sawdust). Indonesia in 2003 had timber waste potency of about 3-4 millions m3. However, ethanol production from sawdust has problems due to its lignin content. Therefore, research on bio-delignification treatment of sawdust prior to ethanol making process is required. In the present study ethanol was produced by simultaneous saccharification and fermentation (SSF) using crude cellulose from Trichoderma viride and Saccharomyces cerevisiae. The raw materials for ethanol production are sengon (Paraserianthes falcataria (L.) Nielsen syn.), meranti (Shorea sp.) and teak (Tectona grandis LIIVN.f.) sawdust after pretreatment with white rot fungi Phanerochaete chrysosporium for 10, 20 and 30 days incubation time. The yield of ethanol was between 1.65-44.83 g/1. The best combination treatment is sengon sawdust with 30 day incubation time.

Hidrolisis Media Sisa Budidaya Jamur Kuping Menggunakan Tiga Jenis Enzim Selulase Denny Irawati
Jurnal Ilmu Kehutanan Vol 11, No 1 (2017)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Jamur kuping (Auricularia polytricha) termasuk dalam kelas Basidiomycetes yang banyak dibudidayakan di Indonesia. Setelah 6-8 bulan masa pembudidayaan media budidaya jamur kuping harus diperbaharui. Sejauh ini media sisa budidaya jamur kuping belum dimanfaatkan dengan baik, bahkan limbah tersebut hanya dibuang begitu saja sebagai limbah. Hal ini sangat berbahaya karena sisa miselia yang terdapat di dalam media sisa dapat mendegradasi lebih lanjut media dan mengakibatkan pencemaran lingkungan yaitu terlepasnya gas metana ke udara. Oleh karena itu pemanfaatan sisa media budidaya kuping perlu dilakukan antara lain untuk memproduksi gula pereduksi dengan menggunakan berbagai jenis enzim selulase. Penelitian ini menggunakan media sisa budidaya jamur kuping yang berasal dari 3 jenis kayu, yaitu sengon (Falcataria moluccana), jati (Tectona grandis), dan meranti (Shorea sp.), yang dihidrolisis menggunakan 3 jenis enzim selulase yaitu Driselase, Cellulase “Onozuka” R-10, dan Meicelase. Setelah hidrolisis, kemudian diukur laju hidrolisisnya dan dianalisis kadar gula pereduksinya. Rancangan yang digunakan adalah rancangan acak lengkap dengan aras 3 x 3 serta ulangan sebanyak 3 untuk setiap perlakuan. Hasil penelitian menunjukkan bahwa media sisa budidaya jamur kuping memiliki potensi sebagai bahan baku gula pereduksi yang selanjutnya dapat difermentasi menjadi bio-etanol atau bahan kimia lainnya. Laju hidrolisis berkisar antara 1,43-21,29%, dengan kombinasi tertinggi yaitu media sisa budidaya yang terbuat dari serbuk kayu meranti dengan menggunakan enzim Meicelase. Kadar dan rendemen gula pereduksi tertinggi juga dihasilkan dari kombinasi perlakuan yang sama, yaitu berturut-turut sebesar 127,7 mg/g dan 12,8% dengan kisaran hasil antara 56,01-127,7 mg/g atau rendemen 5,6-12,8%.The Hydrolysis of Ear-Mushroom Cultivation Media Residue by Using Three Kinds of Cellulase EnzymesAbstractEar mushrooms (Auricularia polytricha) belongs to class Basidiomycetes is widely cultivated in Indonesia. After 6-8 months of cultivation, the media should be renewed. Therefore, the rest of the media is under utilized and just thrown away as a waste. This is dangerous because the residual mycelia that contained in the rest of the media may further degrade the media. This pollutes the environmental pollution due to discharge of methane into the air. Therefore, the utilization of residual ear mushroom media needs to be processed. In fact, this residue can produce a reducing-sugar using various commercial cellulase enzymes. This study used the residual media of the cultivation of mushroom derived from three types of wood, i.e. sengon (Falcataria moluccana), teak (Tectona grandis), and meranti (Shorea sp.), and then they were hydrolyzed using three types of cellulase enzymes, i.e. Driselase, Cellulase “Onozuka” R-10, and Meicelase. The hydrolysis rate was measured and the reducing-sugar yield was analyzed. The design used was completely randomized design with 3 x 3 factors and 3 replications for each treatment. The results showed that the residue of the mushroom cultivation media has the potential of reducing sugar as a raw material which can then be fermented into bio-ethanol or other chemicals. Hydrolysis rate ranged from 1.43 to 21.29%. The highest combination of residual cultivation medium was made from meranti sawdust by using Meicelase enzyme. The highest reducing sugar content yield were also resulted from the combination of the same treatment, which amounted to 127.7 mg/g or 12.8% with output range between 56.0 and 127.7 mg/g (or yield 5.6-12.8%).

Association of Mushroom Cultivation and Ozonolysis as Pretreatment for Enzymatic Saccharification of Sengon(Falcataria moluccana) Sawdust Denny Irawati; Soekmana Wedatama; Futoshi Ishiguri; Shinso Yokota
Jurnal Ilmu Kehutanan Vol 12, No 1 (2018)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Wood industry based on sengon (Falcataria moluccana) material has developed rapidly and cause the huge wastes. Lignocellulosic materials, such as sengon sawdust, offer a great potential as cheap and abundant feedstock for biofuels production. Ozone oxidation pretreatment was carried out on sengon wastes to improve fermentable sugar production by enzymatic saccharification. Sengon wood (SW), sengon media (SM), and sengon spent media (SMM), that was waste of media after mushroom cultivation, treated with ozone for different treatment time were enzymatically saccharified. Then the decrease of hydrolysis weight, reducing sugar yield, and monosaccharide yields were determined. Ozone treatment resulted in Klason lignin degradation in the all samples, resulting in the improvement of subsequent enzymatic saccharification. Ozone treatment with the duration more than 30 min was not suitable for SMM sample. Glucose yield after saccharification from the ozone-treated sample for 60 min was the highest among all samples in SW and SM treated, but for SMM it was 30 min. Based on these results, we concluded that mushroom cultivation can reduce the ozone time treatment and it is an effective treatment to improve sugar yield by enzymatic saccharification of sengon wastes. Gabungan Pra-perlakuan Ozonolisis dan Budidaya Jamur Sebelum Sakarifikasi Enzimatis Serbuk Kayu Sengon (Falcataria moluccana)IntisariIndustri kayu berbahan baku kayu sengon (Falcataria moluccana) saat ini tengah berkembang dengan sangat pesat dan hal ini menyebabkan timbulnya banyak limbah kayu sengon. Materi lignosellulosa, seperti limbah serbuk kayu sengon, merupakan potensi yang sangat baik sebagai bahan baku bio-fuel karena murah dan banyak tersedia. Praperlakuan dengan oksidasi ozon pada limbah kayu sengon dilakukan untuk meningkatkan produksi gula reduksi melalui proses sakarifikasi enzimatis. Serbuk sengon (SW), media sengon (SM), dan limbah media sengon (SMM), yang merupakan sisa dari media budidaya jamur, diberi perlakuan dengan ozon pada berbagai lama waktu perlakuan. Sampel yang sudah diberi perlakuan ozon tersebut kemudian dianalisis kandungan kimianya dan di-sakarifikasi secara enzimatis. Selanjutnya diukur laju hidrolisis, kadar gula pereduksi, dan kadar monosakarida. Perlakuan dengan ozon menyebabkan degradasi kadar Klason lignin di semua sampel, sehingga mengakibatkan peningkatan kemampuan sakarifikasi dari enzim. Perlakuan ozon dengan lama waktu lebih dari 30 menit tidak sesuai untuk sampel SMM. Kadar glukosa hasil sakarifikasi yang tinggi diperoleh dari sampel SW dan SM yang diberi perlakuan ozon selama 60 menit, serta SMM yang diberi perlakuan 30 menit. Berdasarkan hasil ini, dapat disimpulkan bahwa perlakuan jamur dapat mengurangi lama waktu perlakuan ozon dan praperlakuan efektif untuk meningkatkan kadar gula reduksi yang dihasilkan dari sakarifikasi enzimatis limbah kayu sengon.

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