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Deteksi methicillin resistant Staphylococcus aureus pada perokok melalui pemeriksaan kultur apus tenggorokan Ni Wayan Desi Bintari; Putu Ayu Parwati
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15310

Abstract

Perilaku merokok berkorelasi positif dengan meningkatnya angka kejadian infeksi oleh Methicillin Resistant Staphylococcus aureus atau dikenal dengan MRSA. Perokok dilaporkan memiliki tingkat kolonisasi MRSA lebih tinggi dibandingkan non-perokok. Hal tersebut akan sangat berbahaya karena infeksi MRSA dapat menyebabkan infeksi parah seperti infeksi aliran darah, infeksi paru-paru atau necrotising fasciitis (kerusakan jaringan). Penelitian ini bertujuan untuk melakukan deteksi awal keberadaan MRSA yang berasosiasi dengan perokok aktif. Responden pada penelitian ini adalah perokok aktif yang secara rutin melakukan konseling di Klinik Berhenti Merokok (KBM) Puskesmas 1 Denpasar Utara yang memenuhi kriteria inklusi dan eksklusi. Pengujian bakteriologi dilakukan di Laboratorium STIKES Wira Medika Bali dengan melakukan kultur terhadap spesimen apus tenggorokan responden. Identifikasi MRSA dilakukan dengan melakukan pengujian sensitivitas dengan metode Kirby Bauer menggunakan cakram antibiotik oxacillin OX 1 µg. Hasil isolasi dan identifikasi S. aureus pada sampel apus tenggorokan 13 responden didapatkan hasil sebanyak 4 isolat (30,8%) teridentifikasi sebagai S. aureus sedangkan sebanyak 9 (69,2,8%) sampel menunjukkan hasil negatif. Hasil uji sensitivitas terdapat 2 isolat yang memiliki daya hambatan intermediate yaitu SA1 dan SA2 dengan diameter daya hambatan rata-rata sebesar 12 mm dan 11,83 mm. Berdasarkan hasil tersebut disimpulkan bahwa tidak ditemukan bakteri MRSA pada sampel usap tenggorokan perokok aktif pasien konseling di KBM Puskesmas 1 Denpasar. 
Suplementasi Soya Yogurt Untuk Perbaikan Kelimpahan Mikroba Sekum (Bakteri Asam Laktat Dan Escherichia coli) Tikus Diabetes Soya Yogurt Ni Wayan Desi Bintari; Putu Ayu Parwati
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p04

Abstract

Intestinal microbial dysbiosis is one of the triggers for metabolic syndrome. The increase in pathogenic bacteria triggers the high release of lipopolysaccharides which underlie the incidence of insulin resistance in diabetes mellitus. It is important to modulate the intestinal microbiota towards eubiosis through consumption of probiotics. A potential probiotic product is soy yogurt which contains live bacteria and high protein and isoflavones. The aim of this study was to examine the effect of soy yogurt supplementation on the abundance of cecum microbes (Lactic Acid Bacteria (LAB) and Escherichia coli) in diabetic rats. The research was conducted at the Laboratory of Stikes Wira Medika Bali. Type of research is experimental with a completely randomized design consit of 6 groups. The conditioning of diabetes mellitus was done by induction of nicotinamide (NA) 230 mg / kgBW and streptozotozin (STZ) 65 mg / kgBW. Abundance of cecum microbes analysis using Total Plate Count. The results showed soya yogurt supplementation in rat with diabetes mellitus had a positive correlation in improving the abundance of microbial cecum. The total abundance of LAB in diabetic rat was 7.36 ± 0.15 Log10 CFU/mL and was significantly different (p <0.05) compared to diabetic rat treated with soy yogurt supplementation, namely as much as 8.08 ± 0.1 Log10 CFU/ml. Meanwhile, the abundance of E. coli in diabetic rat was 9.42 ± 0.12 Log10 CFU/mL and was significantly different (p <0.05) compared to rats treated with soy yogurt supplementation, namely 8.14 Log10 CFU/ml.
Beneficial Effects of Soygurt Intake in Type 2 Diabetes Mellitus in Animal Model Rat (Rattus Norvegitus) Ni Wayan Desi Bintari; Putu Ayu Parwati
Medical Laboratory Technology Journal Vol 6, No 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v6i1.258

Abstract

Type 2 Diabetes Mellitus (T2DM) is the more common type of diabetes results from the ineffective use of insulin. Improvement of the metabolic system in T2DM patients can be done through the regulation of gut microbiota balance. Gut microbial improvement can be modulated directly by probiotic food consumption. Soygurt is probiotic food with a low glycemic index (GI) and glycemic load (GL) value and rich in isoflavones, which has a potential effect in reducing diabetes risk. The aim of this study is to determine the effect of soygurt consumption in blood glucose levels and body weight of albino wistar rats (Rattus norvegitus). Reseach using a completely randomized design for experimental study. Subjects of this research are 30 male rats (R. norvegistus) aged 2-3 months with average body weight 150-200 gr. Diabetic rats were induced by using single intraperitoneal injection (175 mg/kg BW) alloxan monohydrate. Soygurt feeding given once daily using oral gavage feeding. The result showed that soygurt feeding in diabetic rats with three variations of treatment could significantly (p