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All Journal Journal of Enviromental Engineering and Sustainable Technology JPPUMA: Jurnal Ilmu Pemerintahan dan Sosial Politik Universitas Medan Area Jurnal Kebidanan Akademi Kebidanan Jember (JKAKJ) Jurnal Kesehatan dr. Soebandi
Noorhamdani Noorhamdani
Universitas Brawijaya
Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : Journal of Enviromental Engineering and Sustainable Technology

 1 
MICROENCAPSULATION OF Cytrus hystrix OIL AND ITS ACTIVITY TEST AS AN ANTIMICROBIAL AGENT Warsito Warsito; Noorhamdani Noorhamdani; Sukardi Sukardi; Rissa Dwi Susanti
Journal of Environmental Engineering and Sustainable Technology Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (729.465 KB) | DOI: 10.21776/ub.jeest.2017.004.02.9

Abstract

Microencapsulation Kaffir lime oil (C. hystrix DC.) has advantages such as capable of controlling the evaporation process, oxidation by air or oxygen and release the active ingredient is stable, thus providing a constant concentration over long periods of therapy. In this research microencapsulation of kaffir lime oil from leaves (MJP-D) and rind (MJP-KB) using chitosan to crosslinking with glutaraldehyde (ratio 2:1). Characterization of kaffir lime oil use Gas chromatography-mass spectrometry (GC-MS), characterization of microcapsule use FTIR and particle size analyzer, while analysis of essential oil content in the microcapsules with UV-Vis spectroscopy. Composition of MJP-D consist of citronellal (85,07%), lonalool (3,47%), citronelil acetate (2,77%) and sabinene (2,79%), while MJP-KB consist of citronellal (20,91%), terpinen-4-ol (11,93%), a-pinene (21,44%) and limonene (12,59%). Microcapsul MJP-D and MJP-KB has a distinctive absorption crosslinking results on 1558 cm-1 (-C=N) and particle size 340,43-404,90 nm. The effectiveness microcapsulation of MJP-D and MJP-KB 37,5% and 86,88% with each zone of inhibition against Pseudomonas aeruginosa bacteria is 12,8 mm and 17,1 mm. Keywords: microcapsul, kaffir lime oil, antimicrobial P. aeruginosa
AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA MINYAK JERUK PURUT (Citrus hystrix DC.) DAN KOMPONEN UTAMANYA Warsito Warsito; Noorhamdani Noorhamdani; Sukardi Sukardi; Suratmo Suratmo
Journal of Environmental Engineering and Sustainable Technology Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.34 KB) | DOI: 10.21776/ub.jeest.2017.004.01.3

Abstract

Kaffir lime oil (C. hystrix DC.) is consist of a complex mixture of hydrocarbons terpenoids and terpenoids which contain oxygen are distributed in every part of the plant. In this research tested the antioxidant and antimicrobial activity, both against kaffir lime oil from twigs, leaves and rind including the main components citronellal. The antioxidant was evaluated by testing its ability to catch DPPH radical. Antimicrobial activity is measured by its ability to inhibit the growth of gram-negative bacteria, Escheria coli. Gas chromatography-mass spectrometry (GC-MS) is used to analyze chemical composition of kaffir lime oil. Value of IC50 of kaffir lime oil obtained was 6,43 (mL/mL); 6,83 (mL/mL); 9,48 (mL/mL) and 10,1 (mL/mL), consecutive to kaffir lime oil from rind, leaves, twig-leaf mixture and citronellal. Compared to the other kaffir lime oil and major components citronellal, kaffir lime oil from rind show the most vigorous activity in inhibiting growth of Escheria coli bacteria, by MIC values of 12,5 (mL/mL). GC-MS analysis showed that citronellal and citronellol as major component on kaffir lime oil from leaves and twig-leaf mixture, while the major component of kaffir lime oil from rind is b-pinene, limonene, citronellal dan terpinen-4-ol. Keywords: kaffir lime oil, citronellal, citronellol, antioxidant, antimicrobial
Co-Authors Dwi Yuni Nur Hidayati Hening Ryan Aryani Istifadatul Ilmiya Mukhamad Fathony Novi Budi Nur Rohmah Prihantanti Rissa Dwi Susanti Sri Poeranto Sujarwo Sujarwo Sukardi Sukardi Suratmo Suratmo Warsito Warsito