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All Journal Jurnal Analis Kesehatan Lontara: Journal of Health Science and Technology Lontara Journal Of Health Science And Technology
Andri Sukeksi
Universitas Muhammadiyah Semarang
Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Potensi Ekstrak Etanol Daun Sirih (Piper Betle L.) Sebagai Pengganti ADP (Adenosine Diphosphate) Pada Pemeriksaan Agregasi Trombosit Andri Sukeksi; Rizqi Yogania Rahmawati
Lontara Journal of Health Science and Technology  Vol 2 No 1 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v2i1.184

Abstract

Adenosine diphosphate (ADP) reagent is often used in the examination of platelet aggregation, yet has limitations in terms of price and availability. Natural source namely betel leaf (Piper betle L.) are believed to be used as a substitute for ADP because betel leaf have the characteristics of styptic (to resist bleeding), vulnerary (to heal skin wounds), and anti-inflammatory (to preserve inflammation) (Moeljanto & Mulyono, 2003). The aim of this study was to determine the potential of betel leaf on the examination of platelet aggregation. The method used in this research included the betel leaf extraction using ethanol as solvent with an evaporator. The extract obtained was used as a substitute for the ADP reagent in platelet examination in the form of determining the percentage of platelet aggregation in 16 human blood samples, with a population of students at the University of Muhammadiyah Semarang. The final results showed that the addition of betel leaf extraction to the 16 blood samples examined gave an average value of platelet aggregation percentage which was higher (65%) than the mean value of platelet aggregation using ADP reagent mixing (57%). Both results fall within the normal value range, namely 50-70%. It can be concluded that betel leaf extract has the potential to be used as a replacement reagent for ADP in the process of examining platelets, especially in determining the percentage value of platelet aggregation.
Pengaruh Sentrifugasi Spesimen Darah Anemia Terhadap Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Slide Sri Puji Handayani; Andri Sukeksi; Muhammad Ardi Afriansyah
Jurnal Analis Kesehatan Vol 11, No 1 (2022): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v11i1.3196

Abstract

Anemic patients often have low hemoglobin and red blood cell level, which on the slide method and ABO system blood type test is used to obtain low degree of agglutination. Addition of treatment of a centrifugation was used to obtain a stronger degree of agglutination. The purpose of this research was to determine the difference degree of agglutination between centrifuged and uncentrifuged blood specimens in anemic patients. Quasi experimental research with non-equivalent control group design was conducted in March 2022 at the Hematology Laboratory, Universitas Muhammadiyah Semarang. The research sample were 16 specimens of anemic patients for slide method and ABO system blood type test. All samples were checked for blood type testbefore being centrifuged, then re-checked after the centrifugation there only concentrated red blood cell deposit were taken with only a small amount of plasma. The data from the test were analyzed using the Kruskal Wallis method. Comparison anti-A, anti-B, and anti-AB between centrifuged and uncentrifuged blood specimens there was a significant difference (p value < 0,005 means p = 0,002). The result showed that there were effect by centrifugation for the degree of agglutination improved better in centrifuged if compared with uncentrifugedanemicpatients blood specimens on blood type test slide method. 
Potensi Ekstrak Etanol Daun Sirih (Piper Betle L.) Sebagai Pengganti ADP (Adenosine Diphosphate) Pada Pemeriksaan Agregasi Trombosit Andri Sukeksi; Rizqi Yogania Rahmawati
Lontara Journal of Health Science and Technology Vol 2 No 1 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v2i1.184

Abstract

Adenosine diphosphate (ADP) reagent is often used in the examination of platelet aggregation, yet has limitations in terms of price and availability. Natural source namely betel leaf (Piper betle L.) are believed to be used as a substitute for ADP because betel leaf have the characteristics of styptic (to resist bleeding), vulnerary (to heal skin wounds), and anti-inflammatory (to preserve inflammation) (Moeljanto & Mulyono, 2003). The aim of this study was to determine the potential of betel leaf on the examination of platelet aggregation. The method used in this research included the betel leaf extraction using ethanol as solvent with an evaporator. The extract obtained was used as a substitute for the ADP reagent in platelet examination in the form of determining the percentage of platelet aggregation in 16 human blood samples, with a population of students at the University of Muhammadiyah Semarang. The final results showed that the addition of betel leaf extraction to the 16 blood samples examined gave an average value of platelet aggregation percentage which was higher (65%) than the mean value of platelet aggregation using ADP reagent mixing (57%). Both results fall within the normal value range, namely 50-70%. It can be concluded that betel leaf extract has the potential to be used as a replacement reagent for ADP in the process of examining platelets, especially in determining the percentage value of platelet aggregation.
Co-Authors Muhammad Ardi Afriansyah Rizqi Yogania Rahmawati Sri Puji Handayani