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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Abdi Insani Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Bastian Bastian
Institut Ilmu Dan Kesehatan Teknologi Muhammadiyah Palembang
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Perbedaan Kadar Trigliserida Pada Sampel Darah Segera Disentrifugasi Dan Sampel Darah Dibekukan Selama 20 Menit Sebelum Disentrifugasi Reni Junika Familianti; Indah Sari; Bastian Bastian
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.9580

Abstract

Trigliserida atau yang sering disebut triasilgliserol adalah salah satu jenis lemak yang terdapat dalam darah dan berbagai organ tubuh yang dibentuk dari gliserol dan lemak yang ada dalam makanan yang dikonsumsi secara berlebihan. Nilai trigliserida yang tinggi memiliki kaitan dengan timbulnya penyakit serangan jantung, stroke dan diabetes. Penelitian ini bertujuan untuk mengetahui perbedaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan selama 20 menit yang dilakukan di Balai Besar Laboratorium Kesehatan Palembang. Jenis penelitian yang digunakan adalah Cross sectional komparatif, dengan rancangan penelitian intact group comparison. Sampel berupa 30 serum segera disentrifugasi dan 30 serum dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Penelitian ini dilakukan mulai dari persiapan sampel, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil penelitian. Kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel serum segera disentrifugasi yaitu 0.826 mmol/L sedangkan kadar rata-rata pemeriksaan trigliserida yang diperoleh dari sampel dibekukan 20 menit sebelum disentrifugasi yaitu 0.918 mmol/L. hasil uji dependent t test diketahui bahwa nilai signifikan adalah p = 0,342. Nilai p yang didapatkan adalah p > 0,05. Hasil penelitian dapat disimpulkan bahwa tidak ada perbedaan hasil pemeriksaan kadar trigliserida pada sampel darah segera disentrifugasi dan sampel darah dibekukan terlebih dahulu selama 20 menit sebelum disentrifugasi. Kata kunci : Kadar Trigliserida, Segera Sentrifugasi, Dibekukan 20 MenitDaftar Pustaka: 31 (2013-2020)
Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C Juwy Trianes; Bastian Bastian; Dewi Hartati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.2919

Abstract

Pneumonia is an infection that causes the largest death in children worldwide (South Sumatra Province), which occupies the 6th position. One of the laboratory re-identification of Klebsiella pneumonia is a multi-antibiotic testing. The high sensitivity antibiotic that is still used is amikacin. The uniqueness of the diameter of the inhibition zone using the antibiotic amikacin is that it is active against most gram-negative bacilli. But one of the several factors that affect the diameter of the inhibition zone is the incubation temperature. The optimum temperature for pathogenic bacteria is 37oC by using an incubator; however, several factors in the use of instruments such as frequent instability and disruption of installation lead to a need of incubation at 25oC. The study aimed to determine the difference in the diameter of the growth inhibition zone of K. pneumoniae after incubation at 37oC and 25oC. This research is an experimental research conducted at the Microbiology Laboratory of IKesT Muhammadiyah Palembang. The sample is K. pneumoniae which will be subjected to gram staining, biochemical tests, followed by a sensitivity test on Mueller Hinton media which is given an amikacin antibiotic disk and incubated at 370C and 250C in order to calculate the diameter of the zone of inhibition for the growth of K. pneumoniae bacteria. The data was analyzed using the alternative Wilcoxon test which obtained a p value of 0.014. The results of this investigation showed that K. pneumoniae incubated at 37°C and 25°C had a significantly different diameter of the growth inhibition zone.
PERBEDAAN DARAH SEGERA DIPERIKSA, DILAKUKAN PENYIMPANAN PADA SUHU 20ºC-25ºC DAN 4ºC-8ºC SELAMA 6 JAM TERHADAP JUMLAH ERITROSIT Ferdi Afriansyah; Bastian Bastian; Indah Sari; Denny Juraijin
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.51

Abstract

Errors that often occur in laboratory examinations are in the pre-analytic stage with an error percentage of 50%-75%. Examination of blood samples to maintain conditions so that they are not damaged, the samples are usually stored at a temperature 4-8oC for several hours to 4-5 days and blood specimens stored temperature of 20-24oC for a maximum examination 24 hours. However, the examination of the number of erythrocytes should be carried out immediately after collection without storage because the delay in examination time can affect the number of erythrocytes, the longer the delay, the number of erythrocytes decreases because the cells undergo hemolysis, biochemical, biomechanical and immunological reactions. This researcher aims to determine the difference in blood immediately examined, blood stored temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes. The research design used a true experiment with a pre-test design and a post-test control group design with a hypothesis test using the Two-way ANOVA test. This research was conducted 30 respondents of DIV Medical Laboratory Technology students. The results of the study the number of erythrocytes in the blood immediately with an average value of 4.57 million/µI, at a temperature of 20oC-25oC has an average value of 4.65 million/µL, temperature of 4oC-8oC has an average value of 4.50 million/µL and Friedman test obtained p value = 0.172. Conclusion of the study showed that there was no difference in blood immediately examined, the blood was stored at a temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes.
Edukasi Pemeriksaan Kadar CRP Dengan Sampel Darah Kapiler Pada Petugas Laboraturium RS MMC Palembang Bastian Bastian; Feronica Putri Pratama; Faaizah Ramadhania
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 2 No. 6 (2022): November 2022 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25008/altifani.v2i6.288

Abstract

Clinical laboratory examination is a system that can determine decisions regarding a disease diagnosis or monitoring of healing through laboratory results. An examination that can help diagnose and detect abnormalities in the body. One of the parameters for examining inflammation markers is C-Reactive Protein (CRP). In the CRP examination, laboratory officers are still using blood samples. However, in addition to venous blood, capillary blood samples can also be used specifically in infant and pediatric patients where CRP levels have the same results as venous blood. As an effort faced by MMC Hospital laboratory workers regarding CRP examination using capillary blood, socialization and education were carried out so that they could increase knowledge and understanding of MMC Hospital laboratory workers. Explanation of material regarding CRP examination using Venous Blood through poster media, and demonstrating at the end of the activity. Keywords: C-Reaktive Protein, Capillary Blood, Inflammation, Laboratory
Perbedaan Kadar C-Reaktive Protein (CRP) Pada Sampel Serum dan Plasma K3EDTA Dengan Metode Imunoturbidimetri Helniasari Helniasari; Nurhidayanti Nurhidayanti; Bastian Bastian
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.13350

Abstract

Examination of levels of C-Reactive Protein (CRP) in the blood is one of the tests. that can detect inflammation at an early stage. C-Reactive Protein (CRP) is an inflammatory marker that is synthesized in the liver. CRP levels increase due to trauma, bacterial infection and inflammation (inflammation and tissue damage). CRP is synthesized by the liver and It released into the bloodstream for 6-10 hours after the acute inflammatory process and acute destruction. Tissue detection CRP is also one of several proteins often referred to as the acute phase and It is used to monitor changes in the acute inflammatory phase associated with many infectious and autoimmune diseases. Examination of C-Reactive Protein (CRP) used serum and plasma samples of K3EDTA. C-Reactive Protein levels were measured using the Immunoturbidimetric method. This type of research was cross sectional. The study was conducted at the Clinical Pathology Laboratory, Faculty of Science and Technology, Institute of Health Sciences and Technology Muhammadiyah Palembang on December 21, 2021 with samples were 30. The results of the study had an average value in serum samples of 6.3 mg/L and an average value in plasma. K3EDTA 6.5 mg/L. Wilcoxon test results obtained p value = 0.000. The conclusion of the study was that there were differences in the examination of C-Reactive Protein (CRP) levels in serum and plasma samples of K3EDTA using the immunoturbidimetric method.
Perbedaan Hasil Derajat Aglutinasi Serum Grouping Tube Test Dengan Suspensi Reagen NaCl 0,9% Siap Pakai dan Suspensi Reagen NaCl 0,9% Dari Garam Dapur Hani Ammariah; Nurhidayanti Nurhidayanti; Bastian Bastian; Trimin Kartika
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Vol. 19 No. 2 (2022): Sainmatika : Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v19i2.9500

Abstract

ABO blood group examination is a simple laboratory test performed to determine a person’s blood group. There are two types of ABO blood group test, namely cell grouping and serum grouping. Serum grouping examination is very important to prevent incompatible transfusion reactions and organ transplant rejection. Serum grouping was carried out using 10% saline wash suspension of cell A, B and O. The blood cell washing stage was used to remove fragile cells and interfering substances that could result in errors in the examination. The main ingredient used in the blood cell washing procedur is 0.9% NaCl. This study aims to determine the degree of agglutination serum grouping tube test with ready to use 0.9% NaCl reagent suspension and 0.9% NaCl reagent suspension from table salt. The type of research is an experiment. The study was conducted at UDD PMI South Sumatra Province on 30 December 2021 to 5 January 2022 with a totalsample of 67 samples. The results showed that the average degree of agglutination the serum grouping tube test with 0.9% NaCl reagent ready to use was 3.73 and the average degree of agglutination the serum grouping tube test with0.9% NaCl reagent from table salt was 3.69. Wilcoxon test results obtained p value = 0.450. The conclusion of the study, that there is no differences in the results of the agglutination serum grouping tube test with ready to use 0.9% NaCl reagen suspension and 0.9% NaCl reagent suspension from table salt. The table salt showed that 0.9% NaCl reagent can be used as alternative reagent NaCl 0.9% ready to use in the examination of Iserum grouping tube test.
PERBEDAAN JUMLAH KOLONI JAMUR Candida albicans PADA MEDIA Sabouraud Dextrose Agar (SDA) DAN MEDIA MODIFIKASI BIJI NANGKA (Artocarpus heterophyllus lamk) Anjas Prayoga; Bastian Bastian; Aristoteles Aristoteles
Journal of Indonesian Medical Laboratory and Science Vol 4 No 1: April 2023
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v4i1.142

Abstract

Candida albicans is a fungus that lives as normal flora in certain parts of the human body which can instantly become pathogenic if the patient experiences changes or decreases in body resistance. One of the diagnoses for the Candida albicans fungus is culturing, namely isolating it on gowth media such as Sabouraud Dextrose Agar (SDA), but in some areas, it is difficult to use this medium because the price is quite expensive. Jackfruit seeds contain many nutrients needed by fungi such as protein, carbohydrates, and other minerals so that they can gow the Candida albicans fungus, this study aims to determine the difference in the number of Candida albicans colonies on SDA media and jackfruit seed modification media. The research method used a pure experiment. This research was conducted at the microbiology laboratory of the IKesT Muhammadiyah Palembang with a total of 32 samples. The results showed that the average number of colonies of Candida albicans on SDA was 112 CFU/mL media and jackfruit seed modified media 156 CFU/mL. Wilcoxon test obtained a p-value of 0.05 with a value of 0.000. This study concludes that there were differences between SDA media and jackfruit seed modification media.
EDUKASI PEMANFAATAN POOLED SERA SEBAGAI BAHAN KONTROL ALTERNATIF UNTUK PEMANTAPAN MUTU INTERNAL DI LABORATORIUM Bastian; Maria Ulva
Jurnal Abdi Insani Vol 11 No 1 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i1.1459

Abstract

Quality control is a series of checks performed to evaluate the testing process. Increased automation in the clinical laboratory field has increased the need for QC materials for laboratory performance monitoring. One of them is in the Clinical Chemistry laboratory where before conducting an examination, calibration of equipment must be carried out using commercial control materials. Commercial control serum can be purchased at a high price. Thus, it requires homemade alternative control materials in the form of pooled sera. The purpose of the activity is to provide knowledge about the importance of utilizing pooled sera as an alternative control material for strengthening internal quality in the laboratory. The activity method used is socialization and education through poster media with the aim of increasing knowledge and understanding in officers about the importance of utilizing pooled sera as an alternative control material for strengthening internal quality in the laboratory. There is an understanding and improvement in the results of education on laboratory staff with a pretest value of 45.75 and a posttest of 85.83. Laboratory RSUD Kayuagung have understood the utilization of pooled sera as an alternative control material for internal quality assurance in the laboratory.
Co-Authors Anjas Prayoga Aristoteles Denny Juraijin Dewi Hartati Faaizah Ramadhania Ferdi Afriansyah Feronica Putri Pratama Hani Ammariah Helniasari Helniasari Indah Sari Indah Sari Juwy Trianes Maria Ulva Nurhidayanti Nurhidayanti Reni Junika Familianti Trimin Kartika