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Journal : Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)

PERBEDAAN DARAH SEGERA DIPERIKSA, DILAKUKAN PENYIMPANAN PADA SUHU 20ºC-25ºC DAN 4ºC-8ºC SELAMA 6 JAM TERHADAP JUMLAH ERITROSIT Ferdi Afriansyah; Bastian Bastian; Indah Sari; Denny Juraijin
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.51

Abstract

Errors that often occur in laboratory examinations are in the pre-analytic stage with an error percentage of 50%-75%. Examination of blood samples to maintain conditions so that they are not damaged, the samples are usually stored at a temperature 4-8oC for several hours to 4-5 days and blood specimens stored temperature of 20-24oC for a maximum examination 24 hours. However, the examination of the number of erythrocytes should be carried out immediately after collection without storage because the delay in examination time can affect the number of erythrocytes, the longer the delay, the number of erythrocytes decreases because the cells undergo hemolysis, biochemical, biomechanical and immunological reactions. This researcher aims to determine the difference in blood immediately examined, blood stored temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes. The research design used a true experiment with a pre-test design and a post-test control group design with a hypothesis test using the Two-way ANOVA test. This research was conducted 30 respondents of DIV Medical Laboratory Technology students. The results of the study the number of erythrocytes in the blood immediately with an average value of 4.57 million/µI, at a temperature of 20oC-25oC has an average value of 4.65 million/µL, temperature of 4oC-8oC has an average value of 4.50 million/µL and Friedman test obtained p value = 0.172. Conclusion of the study showed that there was no difference in blood immediately examined, the blood was stored at a temperature of 20oC-25oC and 4oC-8oC for 6 hours on the number of erythrocytes.
PERBEDAAN JUMLAH KOLONI JAMUR Candida albicans PADA MEDIA Sabouraud Dextrose Agar (SDA) DAN MEDIA MODIFIKASI BIJI NANGKA (Artocarpus heterophyllus lamk) Anjas Prayoga; Bastian Bastian; Aristoteles Aristoteles
Journal of Indonesian Medical Laboratory and Science Vol 4 No 1: April 2023
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v4i1.142

Abstract

Candida albicans is a fungus that lives as normal flora in certain parts of the human body which can instantly become pathogenic if the patient experiences changes or decreases in body resistance. One of the diagnoses for the Candida albicans fungus is culturing, namely isolating it on gowth media such as Sabouraud Dextrose Agar (SDA), but in some areas, it is difficult to use this medium because the price is quite expensive. Jackfruit seeds contain many nutrients needed by fungi such as protein, carbohydrates, and other minerals so that they can gow the Candida albicans fungus, this study aims to determine the difference in the number of Candida albicans colonies on SDA media and jackfruit seed modification media. The research method used a pure experiment. This research was conducted at the microbiology laboratory of the IKesT Muhammadiyah Palembang with a total of 32 samples. The results showed that the average number of colonies of Candida albicans on SDA was 112 CFU/mL media and jackfruit seed modified media 156 CFU/mL. Wilcoxon test obtained a p-value of 0.05 with a value of 0.000. This study concludes that there were differences between SDA media and jackfruit seed modification media.