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All Journal Journal of Nutrition College Vektora : Jurnal Vektor dan Reservoir Penyakit Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat
Nova Pramestuti
Balai Penelitian dan Pengembangan Kesehatan Banjarnegara
Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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The Natural Rodenticidal Effect of Papain Papaya (Carica papaya L.) in Histopathological Changes of Albino Mice Organs Nova Pramestuti; Corry Laura Junita Sianturi; Eva Lestari; Siwi Pratama Mars Wijayanti
Kesmas Indonesia Vol 11 No 2 (2019): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.386 KB) | DOI: 10.20884/1.ki.2019.11.2.1636

Abstract

Reports on the rodenticide effect of papain papaya to control rats and mice is scarce. The aim of this study was to determine pathological changes in albino mice internal organs (gaster, kidney, liver and intestine) associated with toxicity effect of papain papaya (Carica papaya L.). Seventy-eight adult BALB/c mice of both sexes were divided into 5 groups: 1 control group and 4 treatment groups that were given papain feeding block. Feed intake and mortalities have been monitored in previous study. At the end of the experimental period, tissues of mice were harvested for necropsy and histopathological examination. The abnormality of post mortem examination were hepatomegaly, gastro-intestinal bleeding. Dark discoloration was founded in liver and gaster. Histopathological changes founded in all treatment albino mice were necrosis in gaster, kidney, liver and intestine. Cloudy swelling occurred in liver and dilatation in kidney. Fatty degeneration occured in gaster, liver and intestine. In addition, the kidney and liver were congested. Feed intake of 16 to 32% papain natural rodenticide gave effect to the organs of studied mice, i.e gaster, kidney, liver and intestine.Reports on the rodenticide effect of papain papaya to control rats and mice is scarce. The aim of this study was to determine pathological changes in albino mice internal organs (gaster, kidney, liver and intestine) associated with toxicity effect of papain papaya (Carica papaya L.). Seventy-eight adult BALB/c mice of both sexes were divided into 5 groups: 1 control group and 4 treatment groups that were given papain feeding block. Feed intake and mortalities have been monitored in previous study. At the end of the experimental period, tissues of mice were harvested for necropsy and histopathological examination. The abnormality of post mortem examination were hepatomegaly, gastro-intestinal bleeding. Dark discoloration was founded in liver and gaster. Histopathological changes founded in all treatment albino mice were necrosis in gaster, kidney, liver and intestine. Cloudy swelling occurred in liver and dilatation in kidney. Fatty degeneration occured in gaster, liver and intestine. In addition, the kidney and liver were congested. Feed intake of 16 to 32% papain natural rodenticide gave effect to the organs of studied mice, i.e gaster, kidney, liver and intestine.
DETEKSI VIRUS JAPANESE ENCEPHALITIS PADA MANUSIA DAN VEKTOR DI KABUPATEN TULUNGAGUNG, JAWA TIMUR Nova Pramestuti; Tri Wijayanti; Dyah Widiastuti; Tri Isnani
Vektora : Jurnal Vektor dan Reservoir Penyakit Vol 12 No 1 (2020): Vektora : Jurnal Vektor dan Reservoir Penyakit
Publisher : Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/vk.v12i1.2649

Abstract

Japanese encephalitis is a zoonotic disease transmitted by mosquitoes with pigs as the main reservoir. A small percentage of infected people experience acute encephalitis syndrome, with one in four cases fatal. Recently, the existence of a growing pig population has the potential to increase the risk of JE transmission in Tulung Agung, East Java, Indonesia. The purpose of this study was to detect JE infection in humans and mosquitoes in Tulungagung Regency. A cross-sectional design was conducted from March to October 2016. Human blood screening was carried out in six hospitals in Tulungagung and the mosquitos survey was carried out using human landing collection, resting collection, and light trap around the pig farms in Kaliwungu District. Detection of JE infection was carried out by indirect immuno-linked immunosorbent assay (ELISA) testing of IgM/IgG in human serum samples and Polymerase Chain Reaction (RT-PCR). Transcription reaction in mosquitoes. The ELISA test showed that one of 19 human serum samples was confirmed positive with JE specific IgG. The result of the mosquito survey showed that Anopheles vagus was predominantly collected in Kaliwungu village, while Culex quinquefasciatus was was predominantly species collected in Pulosari Village. The analyses using molecular assays showed that all captured mosquitoes were negative Javanese encephalitis virus (JEV). Abstrak Japanese Encephalitis (JE) merupakan penyakit zoonotik yang ditularkan oleh nyamuk dengan reservoir utama babi. Sebagian kecil orang yang terinfeksi mengalami radang otak (ensefalitis), sekitar satu dari empat kasus berakibat fatal. Peningkatan populasi ternak babi di Kabupaten Tulungagung berpotensi menyebarkan virus JE. Tujuan penelitian untuk mendeteksi infeksi JE pada manusia dan nyamuk di Kabupaten Tulungagung. Penelitian dilakukan pada Bulan Maret-Oktober tahun 2016 dengan desain studi potong lintang. Survey darah manusia dilakukan pada enam rumah sakit di Kabupaten Tulungagung. Survei nyamuk dilakukan satu kali dengan metode umpan badan manusia dan perangkap cahaya, serta penangkapan nyamuk istirahat di sekitar peternakan babi di Kecamatan Kaliwungu. Deteksi infeksi JE dilakukan dengan pemeriksaan Indirect Enzyme-Linked Immunosorbent Assay (ELISA) IgM/IgG pada sampel serum manusia dan Reverse Transcription Polymerase Chain Reaction (RT-PCR) pada nyamuk. Hasil pemeriksaan laboratorium terhadap antibodi IgM/IgG JE menunjukkan satu kasus positif IgG JE dari 19 sampel serum manusia yang diperiksa. Spesies nyamuk yang tertangkap di Desa Kaliwungu didominasi Anopheles vagus, sedangkan di Desa Pulosari didominasi Culex quinquefasciatus. Hasil pemeriksaan RT-PCR terhadap semua sampel nyamuk yang tertangkap menunjukkan negatif virus JE. Satu pasien ditemukan positif antibodi IgG Japanese encephalitis di Kabupaten Tulungagung.
A POOLED DATA ANALYSIS TO DETERMINE RISK FACTORS OF CHILDHOOD STUNTING IN INDONESIA Tri Siswati; Bunga Astria Paramashanti; Nova Pramestuti; Lukman Waris
Journal of Nutrition College Vol 12, No 1 (2023): Januari
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jnc.v12i1.35413

Abstract

Background: Stunting among children remains a public health concern in Indonesia, where the prevalence of stunting in rural areas is higher than in urban areas.Objectives: This study aimed to analyze risk factors for stunting and severe stunting among children aged 0-59 months in rural and urban Indonesia.Method: This cross-sectional study used pooled datasets from Indonesia’s Basic Health Research in 2007, 2010, and 2013. Our study samples included 38,246 children aged 0-59 months whose height-for-age Z-score (HAZ) was between -5.99 and 5.99 standard deviations. The primary outcomes were stunting and severe stunting, whereas dependent variables included factors at the child, household, and community levels. We apply Stata 13 for univariate, bivariate, and multivariate analysis.The prevalence of stunting and severe stunting was higher in rural than urban areas. On one hand, risk factors significantly associated with stunting and severe stunting in urban areas were low birth weight, maternal height, informal father’s occupation, low economic level, and children from East Indonesia and Sumatra. Furthermore, the father’s height was only associated with stunting, while the number of children >3 was only associated with severe stunting. On the other hand, risk factors age, low birth weight, maternal height, father’s height, household member 5-9 people, middle and low economic level were significantly associated with stunting and severe stunting in rural areas. Moreover, poor WASH was only associated with stunting, while informal mother occupation, low father education, number of household members > 9 people, and living in Sumatra and East Indonesia were associated with severe stunting in rural areas.Conclusion: Low birth weight, short parents, and economic income were risk factors for stunting and severe stunting children in urban and rural areas. Keywords: Children; Pooled data; Risk factors; Stunting
Co-Authors Bunga Astria Paramashanti Corry Laura Junita Sianturi Dyah Widiastuti Eva Lestari Lukman Waris Siwi Pratama Mars Wijayanti Tri Isnani Tri Siswati Tri Wijayanti