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EFEK PAPARAN KADMIUM (Cd) TERHADAP FUNGSI GINJAL PEKERJA BENGKEL LAS hernayanti hernayanti; Slamet Santoso; Sri Lestari; Lucky Prayoga; Kamsinah Kamsinah; Rochmatino Rochmatino
Kesmas Indonesia Vol 11 No 1 (2019): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

ABSTRAK Kadmium merupakan salah satu logam berat yang bersifat nefroksik dan membahayakan kesehatan manusia, terutama pekerja bengkel las. Kadmium masuk ke dalam tubuh berikatan dengan metalotionin membentuk ikatan Cd+Mt akan terdeposit di organ ginjal dan menginduksi terbentuknya radikal bebas sehingga mengakibatkan peroksidasi lipid yang dapat merusak organ ginjal, ditandai dengan peningkatan kreatinin dan β2 mikroglobulin. Tujuan penelitian adalah untuk mengetahui efek paparan Cd terhadap fungsi ginjal pekerja bengkel las ditinjau dari parameter kreatinin dan β2 mikroglobulin (β2-M). Rancangan penelitian menggunakan rancangan kasus kontrol. Subyek penelitian terdiri dari 30 orang pekerja bengkel las di Purwokerto dengan masa kerja lebih dari 3 tahun sebagai subyek kasus dan subyek kontrol dari desa Pamijen Baturaden yang mewakili bukan pekerja bengkel las. Data dianalisis menggunakan uji t independent. Hasil penelitian menunjukkan kadar Cd darah, kreatinin darah dan β2 mikroglobulin pada subyek kasus berbeda nyata dibandingkan dengan subyek kontrol. Kadar Cd, kreatinin darah dan β2 mikroglobulin subyek kasus lebih tinggi dibandingkan subyek kontrol. Kesimpulan penelitian paparan Cd berpengaruh terhadap penurunan fungsi ginjal pekerja bengkel las yang ditandai dengan peningkatan kadar Cd darah, kreatinin dan β2-M.

PENAMBAHAN IAA DAN BAP TERHADAP PERTUMBUHAN KALUS EKSPLAN DAUN ANGGREK Coelogyne pandurata Lindl. Johanes De Britto Bintan Cahyo Adi; Kamsinah Kamsinah; Lucky Prayoga
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 2 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.2.4255

Anggrek Coelogyne pandurata Lindl atau anggrek hitam merupakan anggrek endemik dari Kalimantan. Anggrek ini mempunyai keunikan yakni mempunyai sepal berwarna hijau serta labelum yang berwarna hitam. Perbanyakan secara alami tingkat keberhasilannya sangat kecil maka perlu di lakukan kultur in vitro. Salah satu cara kultur in vitro adalah kultur menggunakan eksplan daun untuk memacu pertumbuhan kalus. Pertumbuhan kalus dapat dipacu dengan auksin (IAA) dan sitokinin (BAP). Perpaduan antara konsentrasi auksin dan sitokinin yang seimbang diharapkan bisa memacu pertumbuhan kalus. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan IAA dan BAP terhadap pertumbuhan kalus anggrek C. pandurata Lindl dan mengetahui interaksi IAA dan BAP yang terbaik terhadap pertumbuhan kalus anggrek C. pandurata Lindl. Metode penelitian dilakukan secara eksperimental dengan rancangan acak lengkap menggunakan pola faktorial , faktor 1 kosentrasi IAA dengan 4 taraf : 0, 1, 2, 3 mg/L. Dan faktor ke 2 konsentrasi BAP dengan 4 taraf perlakuan : 0, 1 , 2, 3 mg/L, sehingga ada 16 perlakuan , dan masing-masing perlakuan diulang 3 kali sehingga ada 48 unit percobaan. Parameter yang diamati adalah : waktu munculnya kalus, tebal kalus, jenis kalus, berat kalus dan presentasi terbentuknya kalus. Data yang diperoleh dianalisis menggunakan ANOVA dan dilakukan uji lanjut DMRT dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan pemberian IAA dan BAP dapat mempengaruhi pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. Pengaruh secara interaksi terdapat pada wantu munculnya kalus, tebal kalus dan Prosentase hidup kalus. Serta mampu memacu perkembangan kalus ke arah proliferatif. Sedangkan pemberian IAA secara mandiri dapat memacu penebalan kalus. Interaksi yang terbaik dalam pertumbuhan kalus eksplan daun anggrek Coelogyne pandurata Lindl. adalah A2B2 (IAA 2 mg/L dan BAP 2 mg/L) dan A2B3 (IAA 2 mg/L dan BAP 3 mg/L).

Komposisi Jenis dan Kepadatan Rumput Laut Hydrokoloid berdasarkan Karakteristik Dasar Perairan di Daerah Intertidal Nusakambangan Timur Cilacap Dwi Sunu Widyartini; Achmad Ilalqisny Insan; Kamsinah kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3568

ABSTRACT Hydrocolloid seaweed is seaweed which contains agar, carrageenan and alginate, which are widely used as industrial raw materials. This study aims to determine the type composition of hydrocolloid seaweed and its density based on the characteristics of the bottom waters in the intertidal area of East Nusakambangan Cilacap. The waters of East Nusakambangan have a central coral beach and a hollow coral beach which has a substrate in the form of sand, coral, volcanic rock, and coral fragments. This study used a survey method and sampling using a transect, a perpendicular line of the coast towards the sea, each transect line was placed with a quadrant plot (1 x 1m2) randomly selected on hard and soft substrates. The results of this study, the coral substrate on the two beaches obtained 2-8 types of hydrocoloid seaweed, with the highest composition of seaweed species of 57.6% on hard substrate with the highest density in the Gracilaria gigas species; 440.6-1239.7 g / m2, Gracilaria gigas has a Dichotomus branching type with a cylindrical talus shape. In the mixed substrate on the coast of Karang Tengah, there were 4 types of Hydrocoloid seaweed with a composition of 46.3% hydrocoloid species with the highest density in the species Padina australis; 387.7g / m2. Padina australis sheet and unbranched talus form Keywords: hydrocolloid, composition, East Nusakambangan, Intertidal

Pengaruh Pemberian Sitokinin Terhadap Pertumbuhan Palea dan Lemma Padi Melaui Kultur In Vitro Kamsinah Kamsinah; Triani Hardiyati; Sugiyono Sugiyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.117

A research has been carried out with the aims to: 1. study the influence of both the kind and concentration of cytokinins on palea and lemma growth in in vitro culture; 2. study the influence of palea and lemma age towards external application of cytokinin in in vitro culture; and3. determine the best palea-lemma age, kind and concentration of cytokinin which resulted the best growth of palea and lemma in in vitro culture. The used experimental method was Completely Randomised Design (CRD) with factorial treatment pattern. The applied treatment consisted of three factors i.e. palea-lemma age (U): (55, 60, and 65 days after planting); kind of cytokinin (S): (BAP and Kinetin); and Cytokinin Concentration (K): (0, 5, 10, and 15 M) with 3 replications. The observed variables were the palea and lemma growth with the parameters were palea-lemma length and width. The results showed that the kind of cytokinin had influenced the growth of palea-lemma in in vitro culture, and kinetin has better influence on the palea-lemma growth. The age of the palea-lemma determined the responsiveness of the palea-lemma towards external application of cytokinin. Older palea-lemma showed less responsive than younger ones towards external application of plant growth regulators. Moreover, the treatment combination (U1S2K2) (10 µM Kinetin applied to 55-day-old rice palea-lemma) had the best effect on increasing the size of palea-lemma of IR 64 rice.

The Effect Of Date Palm Extract (Phoenix dactylifera L.) On Orchid Plantlets (Vanda tricolor Lind) Growth using In Vitro Culture Resita Nursechan; kamsinah kamsinah; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4227

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.

Uji Viabilitas Polen Tanaman Kedelai (Glycine max (L.) Merril) pada berbagai Lama Penyimpanan Hastya Tri Andini; Muachiroh Abbas; Kamsinah kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4595

Soybean (Glycine max (L.) Merril) is one of the most important grain-producing crops after rice, wheat, millet and barley. Pollen is a male gametophyte produced by seed plants. Pollen can be used for plant identification because it has a distinctive structure and ornamentation, besides that it can also be used as a plant breeding agent as a contributor to male parental characters. The character of pollen as parental male is very important in plant breeding. One way to know the quality of pollen is to look at its viability. Pollen viability can be tested by staining method or by germination method. Pollen viability is known to be lost over a certain period of time. The purpose of this study was to determine the effect of storage time on viability of soybean pollen, and to determine the best storage period with the highest viability of soybean pollen. This research was conducted experimentally with a Completely Randomized Design (CRD) consisting of 4 treatments, namely P0, Control. P1, 7 days of storage. P2, 14 days of storage. P3, 21 days of storage. The independent variable is variation in storage time while the dependent variable is pollen viability. The parameters observed were the number of stained pollen, germination capacity, and the length of the germinating pollen tube as evidence that the pollen was viable. The research data obtained were analyzed using Analysis of Variance (ANOVA) with an error rate of 5%, then continued with the BNT test with an error rate of 5%. The results showed that the provision of storage time treatment on soybean pollen had an effect on pollen viability, namely by reducing pollen viability. The best shelf life to get the highest pollen viability in soybean pollen is 0 to 7 days after the sample is taken.

The Effect of Date Palm Extract (Phoenix dactylifera L.) on Orchid Plantlets (Vanda tricolor Lind.) Growth Using In Vitro Culture Resita Nursechan; Kamsinah Kamsinah; iman budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.6874

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.

APLIKASI NAA PADA PROPAGASI KANTONG SEMAR Nepenthes adrianii Batoro, Wartono & Jebb Rosa Amalia Wahdani; Murni Dwiati; Kamsinah Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5481

Nepenthes adrianii is an endemic species of Mount Slamet. The existence of this species has begun to be threatened. This situation encourages efforts to conserve N. adrianii, which is through propagation using stem cuttings of N. adrianii. In order for the propagation of stem cuttings to grow properly, it is necessary to add growth hormone (ZPT) from the auxin group such as NAA (Napthalene Acetic Acid). This research has been conducted to study the effect of NAA and its effective consentration for the growth of N. adrianiistem cuttings. This research was conducted using experimental methods. The experimental design used was a Randomized Completely Block Design (RCBD). The block in this study is total leaves per cuttings. Block 1 is the leafless cuttings, block 2 is cuttings with one leaf, block 3 is cuttings with two leaves, and block 4 is cuttings with three leaves. The NAA concentrations in this study are 0, 5, 10, 15, 20, and 25 ppm. There are a total of 24 experimental units. The variable observed was the growth of N. adrianii stem cuttings. Parameters observed in this study are days taken for sprouting, number of shoots, number of leaves, leaf length, and leaf width. The results were analyzed using Variety Analysis and then analyzed with Tukey's test at the test level of 0.05 at the time of significant treatment. The results showed that the application of NAA did not affect the growth of the stem cuttings of N. adrianii. The best concentration of NAA could not be determined because there was no effect of NAA on the growth of N. adrianii stem cuttings.

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