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All Journal As-syifaa Jurnal Farmasi Majalah Farmaseutik
St. Maryam
Fakultas Farmasi Universitas Muslim Indonesia, Makassar
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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PENELUSURAN FUNGI ENDOFIT SEBAGAI PENGHASIL SENYAWA ANTIBIOTIKA DARI DAUN NANAS (Ananas comosus (L) Meer) Fitriana Fitriana; St. Maryam; Tadjuddin Naid; Maryana Maryana
As-Syifaa Jurnal Farmasi Vol 8, No 1 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.917 KB) | DOI: 10.33096/jifa.v8i1.152

Abstract

A search endophytic fungi from the leaves of pineapple (Ananas comosus (L) Merr) as a producer of antibiotic compounds will be carried out in order to obtain the endophytic fungi that have the ability to produce bioactive compounds useful as antibiotics.. Results of endophytic fungi isolates obtained six fungal isolates (IJA 1, IJA 2, IJA 3, IJA 4, IJA IJA 5 and 6), which have a different macroscopic characterization of forms, edge and elevation colonies. Isolates pure microbial fermentation results in the test antibiotic activity against some bacteria using agar diffusion test. All isolates pure fermentation results showed inhibitory activity against all bacteria in test as Bacillus subtilis, Esherichia coli, Pseudomonas aeruginosa, Salmonella thypi, Shigella dysenteriae, Staphylococcus aureus, Streptococcus mutans, and Vibrio cholerae. The inhibition of fungi isolates shown in (IJA 4) with a 26 mm zone of inhibition against Esherichia coli bacteria.
UJI AKTIVITAS ANTIOKSIDAN ISOLAT FUNGI ENDOFIT DAUN GALING-GALING (Cayratia trifolia L.) DENGAN METODE 1,1-DIPHENYL-2-PICRYLHYDRAZIL (DPPH) Magfirah Abdullah; Fitriana Fitriana; St. Maryam
As-Syifaa Jurnal Farmasi Vol 12, No 2 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v12i2.617

Abstract

Endophytic fungi generally produce secondary metabolites that have beneficial biological activities such as anticancer, antiviral, antibacterial or antioxidant compounds. The aim of this study was to examine the antioxidant activity of galling-galing leaf endophytic fungi isolate (Cayratia trifolia L.) by 1,1-diphenyl-2-picrylhydrazil (DPPH) method. This study used IDGG-03 endophytic fungi isolate fermented for 21 days using MYB medium then extracted and evaporation was carried out. The fermentate extract obtained was then tested qualitatively and quantitatively for antioxidant activity. Qualitative results using TLC plates indicate that galling-leaf fungi isolates have antioxidant activity which is characterized by the presence of yellow spots on a purple background. And in the quantitative test IC50 68,133 µg / mL was obtained, which indicated the antioxidant activity of galling-vane leaf endophytic fungi isolate (Cayratia trifolia L.) classified as a strong antioxidant.
Produksi dan Karakterisasi Gelatin dari Limbah Tulang Ayam dengan Menggunakan Spektrofotometer Ftir (Fourier Transform Infra Red) St. Maryam; Nurmaya Effendi; Kasmah Kasmah
Majalah Farmaseutik Vol 15, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.162 KB) | DOI: 10.22146/farmaseutik.v15i2.47542

Abstract

Gelatin merupakan protein hasil hidrolisis parsial kolagen tulang dan kulit. Gelatin memiliki sifat yang khas, yaitu dapat berubah secara reversible dari bentuk sol ke bantuk gel, mengembang dalam air dingin, dapat membentuk film serta mempengaruhi viskositas suatu bahan, dan melindungi sistem koloid. Penelitian ini bertujuan untuk mengetahui persen rendamen serta menentukan karakteristik proksimat dan fisikokimia dari gelatin tulang ayam. Penelitian ini dilakukan dengan metode eksperimental, proses yang dilakukan untuk menghasilkan gelatin melalui beberapa tahap yaitu degreasing, demineralisasi, ekstraksi dan pengeringan. Kemudian gelatin hasil ekstraksi dari keempat perlakuan tersebut dilakukan beberapa pengujian seperti karakteristik  proksimat  (kadar  air,  abu,  dan protein),  dan  karakteristik  fisikokimia  (pH dan viskositas) serta identifikasi gugus fungsi menggunakan spektrofotometri Fourier Transform Infra Red (FTIR). Hasil penelitian ini menunjukkan persen rendamen gelatin 1,030%, hasil karakterisasi proksimat yaitu kadar air 9,214%, kadar abu 10,396%, kadar protein 67,756% dan hasil karakteristik fisikokimia yaitu pH 6, viskositas relatif 6 cP. Berdasarkan spektra FTIR, gugus fungsi gelatin tulang ayam yang dapat diidentifikasi diantaranya adalah  gugus O-H, N-H, C-N, C=O & C-H. Dari hasil penelitian yang didapatkan dapat disimpulkan bahwa gelatin dari tulang ayam memenuhi standar mutu gelatin.
Co-Authors Fitriana Fitriana Kasmah Kasmah Magfirah Abdullah Maryana Maryana Nurmaya Effendi Tadjuddin Naid