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All Journal Al-Kimia Jurnal Pijar MIPA
Ulul Khairi Zuryati
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Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

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Determination of Ascorbic Acid Content using the Reverse Phase–High Performance Liquid Chromatography (RP-HPLC) Method Nurul Ismillayli; Dhony Hermanto; I. G. A. Sri Andayani; Ruru Honiar; Ulul Khairi Zuryati; Baiq Mariana; Linda Marta Shofiyana
Al-Kimia Vol 8 No 2 (2020): DECEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v8i2.15097

Abstract

A method of reverse phase-high performance liquid chromatography (RP-HPLC) was evaluated in ascorbic acid determination. The determination was conducted at optimum conditions for RP-HPLC method, including ethanol as mobile phase, flow velocity of 0.8 mL/min, running time of 5 minutes, injection volume of 10 µL, UV detector, wavelength of 245 nm with internal standard of ascorbic acid in metaphosphate-acetic acid. Result of measurement was validated by AOAC Official Method 967.21. It was obtained that RP-HPLC method was high sensitive and precision method due to its limit of detection was 0,5 µg/mL and  RSD  was ≤ 2%. The results of the determination of vitamin C content in fresh ceruring fruit using RP-HPLC method and the titrimetry method namely, 52.9057 ± 0.17 and 54.2066 ± 0.87 mg in 100 g sample, respectively. Based on statistical analysis (t-test), there were no significant differences for the two methods used. The percentage of recovery obtained is excellent in the range of 97.9-104.3%. Therefore, the RP-HPLC method is expected to be an accurate method for routine analysis of vitamin C. Key word: ascorbic acid, reverse phase, HPLC, titrimetric. 
APLIKASI TEKNOLOGI NANOKOMPOSIT LIMBAH PADAT PETERNAKAN SAPI (LPPS) DI INDUSTRI GERABAH DESA BANYUMULEK KABUPATEN LOMBOK BARAT Saprini Hamdiani; Ulul Khairi Zuryati; Suthami Ariessaputra
Jurnal Pijar Mipa Vol. 12 No. 2 (2017): September
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.201 KB) | DOI: 10.29303/jpm.v12i2.353

Abstract

Desa Banyumulek Kabupaten Lombok Barat adalah sentra industri gerabah terbesar di Provinsi NTB sejak tahun 1960-an. Gerabah dari Desa Banyumulek bahkan telah mampu menembus pasar ekspor ke beberapa negara Eropa dan Australia. Perkembangan industri gerabah yang semakin positif, tidak didukung oleh ketersediaan bahan baku pembuatan gerabah. Salah satu bahan baku utama pembuatan gerabah yaitu pasir semakin mahal harganya. Penggunaan pasir dapat digantikan oleh limbah padat peternakan sapi (LPPS). Limbah padat peternakan sapi mengandung selulosa yang tinggi sehingga efektif sebagai filler penguat tanah berlempung berdasarkan konsep teknologi nanokomposit. Kandungan silikat pada limbah padat peternakan sapi juga dapat meningkatkan plastisitas tanah berlempung, sehingga gerabah yang dibuat semakin mudah dibentuk. Selain itu, kandungan C-organik yang tinggi akan mengurangi bobot gerabah yang dibuat sampai 24,5 %. Limbah padat peternakan sapi sangat potensial diaplikasikan pada industri gerabah karena ketersediaannya yang sangat melimpah di Provinsi NTB yang terkenal sebagai Bumi Sejuta Sapi (BSS). Oleh karena itu, melalui kegiatan Ipteks Bagi Masyarakat (IbM) dilakukan kegiatan sosialisasi dan praktek pengolahan limbah padat peternakan sapi kepada para pengrajin gerabah. Produk dari kegiatan ini adalah gerabah yang kuat dan ringan, dengan bahan baku alternatif yang ramah lingkungan.
Co-Authors Baiq Mariana dhony hermanto I. G. A. Sri Andayani Linda Marta Shofiyana Nurul Ismillayli Ruru Honiar Saprini Hamdiani Suthami Ariessaputra