Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Biogenesis: Jurnal Ilmiah Biologi
Mashuri Masri
Jurusan Biologi, Fakultas Sains dan Teknologi, UIN Alauddin Makassar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Batang Nanas (Ananas comosus) Berdasarkan Variasi pH Nurhidayah N; Masriany M; Mashuri Masri
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 2 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i2.457

Abstract

This research aims to determine the highest protein content and the optimum activity of the enzyme bromelain extracted from pineapple stem. This research was conducted at the Laboratory of Microbiology Makassar Alauddin State Islamic University in May 2013. Variable in this study was the isolation and measurement of bromelain enzyme activity as the independent variable while the crude extract of pineapple stem by variations in pH as the dependent variable. The type of this research was experiments with bradford method for determination of protein content enzyme bromelain, with ammonium sulfate concentration variation for precipitation was 10-60%, as well asthe determination of the enzyme bromelain activity at variation pH was 4.0; 5.0, 6.0; 7.0 and 8.0, at 650C temperature with incubation time was 10 minutes. Each stage has done three times in repetition and analyzed spectrometry. The results showed the highest levels of protein precipitation with ammonium sulfate at 60% was 37,785 mg/ml. While the optimum pH of the enzyme bromelain activity at pH 6.0 was 1.021 activity units /gram.Keywords: bromelain enzyme, Pineapple stem (Ananas comosus), precipitation with ammonium sulfate, protein contents, pH
Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Bonggol Nanas (Ananas comosus) pada Variasi Suhu dan pH Mashuri Masri
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.478

Abstract

This study was conducted to isolate the bromelain enzyme from pineapple weevil (Ananas comosus) and measure the protein and enzyme activity of bromelain with gelatin substrate. Research stage involves determining the protein content of the bromelain enzyme in the treatment of ammonium sulfate precipitation by 10-60% concentration and determination of bromelain enzyme activity at various pH 4.0; 5.0; 6.0; 7.0 and 8.0, with the incubation time of 10 minutes at a temperature of 650 C. Each stage performed three repetitions and analyzed using spectrometry. The results obtained showed the highest protein content in precipitation with ammonium sulfate 60% in the amount of 37.214 mg / ml and the optimum pH of bromelain enzyme activity at pH 7.0 with the value of the activity of 1,081 units / gram.Keywords: ammonium sulfate precipitation, bromelain enzyme, pineapple weevil (Ananas comosus),pH
Co-Authors Masriany, Masriany NURHIDAYAH NURHIDAYAH