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Bioakumulasi Timbal (Pb) Pada Makroalga Padina australis Hauck Di Perairan Laut Kota Makassar, Sulawesi Selatan Supardi, Wahyu; Nugroho, Andika Puspito
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Pb lead metal is one of marine pollution material that can accumulate on macroalga Padina australis. The purpose of this research is to know the accumulation of Pb on P.australis, the abundance, and to know the Pb content correlation with abundance on P. australis. Research conducted on February 2018 in Makassar marine waters. The results indicate that there is a difference of the metal content of Pb on P.australis in each station. The metal content of Pb on the station I was at the range 0,0964-0,1388 ppm, Station II 0,0496-0,1050 ppm, and Station III 0,0597-0,1035 ppm which has exceeded the waters standard limit set>0.008 ppm.Â The high average Pb concentration on the station I because the station I was closer to the mainland of potentially greater influx of Pb heavy metals on macroalgae. P. australis abundance on a station I 92 individuals, II 152 individuals, and III 319 individuals. The data analysis result shows the value of significant correlations 0.384>0.05 that there is no correlation between the Pb concentrations with abundance but the direction of correlation indicates negative (-0331) which means that if the Pb concentration rises thenÂ P. australis abundance will decrease.

Bioakumulasi Timbal (Pb) Pada Makroalga Padina australis Hauck Di Perairan Laut Kota Makassar, Sulawesi Selatan Supardi, Wahyu; Nugroho, Andika Puspito
Bioma : Berkala Ilmiah Biologi Vol. 22, No 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Pb lead metal is one of marine pollution material that can accumulate on macroalga Padina australis. The purpose of this research is to know the accumulation of Pb on P.australis, the abundance, and to know the Pb content correlation with abundance on P. australis. Research conducted on February 2018 in Makassar marine waters. The results indicate that there is a difference of the metal content of Pb on P.australis in each station. The metal content of Pb on the station I was at the range 0,0964-0,1388 ppm, Station II 0,0496-0,1050 ppm, and Station III 0,0597-0,1035 ppm which has exceeded the waters standard limit set>0.008 ppm.Â The high average Pb concentration on the station I because the station I was closer to the mainland of potentially greater influx of Pb heavy metals on macroalgae. P. australis abundance on a station I 92 individuals, II 152 individuals, and III 319 individuals. The data analysis result shows the value of significant correlations 0.384>0.05 that there is no correlation between the Pb concentrations with abundance but the direction of correlation indicates negative (-0331) which means that if the Pb concentration rises thenÂ P. australis abundance will decrease.

Sintesis dan Aplikasi Nanopartikel Kitosan Sebagai Adsorben Cd dan Antibakteri Koliform Swara Yudhasasmita; Andhika Puspito Nugroho
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3432

Chitosan is a derivative compound of chitin which has a linear polysaccharide composed of β-(1–4)-linked d-glucosamine and N-acetyl-d-glucosamine. This compound is found in shrimp shell. In nanoparticle form, chitosan has a great antibacterial activity and adsorption ability rather than normal form. The aims of this study are to study the effect of concentration chitosan nanoparticle to adsorpt cadmium and its antibacterial effect on coliform. This research was started with synthesis of chitosan nanoparticles using acetic acid 2% and TPP 0,1 %, then the sampel was dried by spray dryer. For cadmium adsorption test was conducted by giving chitosan nanoparticle of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, into Cd solution with the concentration of 7 ppm and was filtered with theWhatman paper number 42 (n=3). Cadmium contents in the filtrates and pellets were analyzed by using AAS. For antibacterial test with Salmonella typhimurium andEscherichia coliwere conducted by disc diffusion method, contained of 0.1, 0.2, 0.3, 0.4, and 0.5 g, respectively, were dissolved in 50 ml of 1% acetic acid. Data were analyzed by using oneway ANOVA followed by LSD. Based on the results, the optimum adsorption of cadmium present in addition of 0.4 g/ 50mL, can reduce Cd concentration by 98,7%. For the antibacterial test, the addition of 0.4 g/50mL is the largest zone of inhibition Salmonella thypimuriumand Escherichia coli.

Akumulasi Mn dalam Cairan Tubuh Ligumia nasuta (Say, 1817) di Sungai Ciputri Bandung Jawa Barat Endah Kartikawati; Andhika Puspito Nugroho
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Ciputri upriver, Bandung, West Java has polluted by heavy metal Mn coming from cow feces in the Cikidang village. The aim of research is to study Mn accumulation in the Eastern Pondmussel Ligumia nasuta (Say, 1817). Mn contents in the water, extra pallial fluid, and hemolymph were determined by Atomic Absorbance Spectrophotometer (AAS). The mussels were collected at day 0, 1, and 6 after transplantation at 3 stations: before, near, and after pollution sources. The results show that the highest Mn accumulation was in the extra pallial fluid (1,21 mmol kg-1 ww) at station 2, day 6. It showed that exposure of Mn to the mussels taken place via water.

Bioakumulasi Ion Tembaga Pada Ikan Nila (Oreochromis niloticus L.) di Instalasi Pengolahan Air Limbah (IPAL), Bantul Dian Yuni Pratiwi; Andhika Puspito Nugroho; Ayi Yustiati
Akuatika Indonesia Vol 4, No 2 (2019): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jaki.v4i2.25260

Penelitian ini bertujuan untuk mengetahui kandungan ion tembaga (Cu) pada kolam fakultatif dan kolam pematangan di IPAL, Sewon, Bantul serta mempelajari kemampuan bioakumulasi ion tembaga (Cu) pada tubuh ikan Nila (Oreochromis niloticus L.). Penelitian ini dilaksanakan di IPAL, Sewon, Bantul dan di Laboratorium Biokimia Fakultas Biologi UGM. Ikan Nila sebagai hewan uji diletakkan di kolam pematangan dan kolam fakultatif IPAL, Sewon, Bantul selama dua minggu. Ikan Nila tersebut kemudian diambil setiap minggu. Sampel berupa otot, hati, dan insang dari ikan nila didestruksi dengan metode digesti basah. Kandungan ion tembaga pada kolam dan ikan nila ditentukan dengan Atomic Absorption Spectrophotometer (AAS). Hasil menunjukkan bahwa kandungan ion tembaga (Cu) di kolam fakultatif dari minggu ke 0 sampai 2 berkisar 0,076-0,088 mg/l, sedangkan di kolam pematangan berkisar 0,024-0,030 mg/l. Nilai ini masih di bawah ambang standar baku mutu. Ion tembaga dapat terkumulasi pada hati dan insang ikan Nila. Ion tembaga lebih banyak terakumulasi di hati dibandingkan insang, dan tidak dapat terakumulasi pada otot ikan nila.

BIOAKUMULASI KADMIUM (Cd) PADA MANTEL, INSANG, dan GINJAL KERANG AIR TAWAR Elongaria orientalis (Lea, 1840) Selvi Ariyunita; Andhika Puspito Nugroho
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Cadmium (Cd) is one of non-essensial metals and toxic in low concentration. Cd polution in freshwater ecosystem from the usage of Cd in many industrial activities can interfere biology system of aquatic animals. Evaluation of heavy metals in freshwater ecosystem can be predicted with accumulation of metals in organism. Mussels are aquatic organism that capable to accumulate metals in their body. The purpose of this research were to study pattern of Cd accumulation in body mussels including mantle, gills, and kidneys. Mussels are exposed to Cd via water (20 µgL-1)for 24 days, followed by 12 days of depuration. On day 0, 1, 6, 12, 18, 24 (exposure time) and day 30, 36 (depuration time), mussels were dissected into mantle, gills, and kidneys. Cd bioaccumulation in all organ was determined by using Atomic Absorbance Spectrophotometer (AAS). The result showed that pattern of Cd bioaccumulation attend to increase in all organs within exposure time and attend to decrease within depuration time. The higest level of accumulation being in kidney, followed by gillsand mantle. Keywords: bioaccumulation, cadmium, freshwater mussel

EFEK LAJU KARBONDIOKSIDA (CO₂) TERHADAP MORFOLOGI DAN LAJU PERTUMBUHAN POPULASI Spirulina platensis (Gomont) [The Effect Of Carbon Dioxide (Co₂) Rate To The Morphology And The Growth Rate Of Spirulina Platensis (Gomont) Population] lutfi Anggadhania; Andhika Puspito Nugroho
Journal Penelitian Kehutanan FALOAK Vol 1, No 2 (2017): Jurnal Penelitian Kehutanan FALOAK
Publisher : Balai Penelitian Dan Pengembangan Teknologi Hasil Hutan Bukan Kayu

ABSTRACTCarbon dioxide pollution occurs due to increase the use of fossil fuels for industry and transportation. Due to deforestry, the absorption of carbon dioxide by terrestrial environment is reduced. This will lead the increased of carbon dioxide absorption by the sea. The absorption of carbon dioxide by the ocean will lead the changes in ocean chemistry and affects the marine ecosystems. Spirulina platensis, as a cosmopolitan organism that can use inorganic carbon that absorbed by the ocean become one of microorganism that has effect by these changes. This research aims are to study the effect of carbon dioxide rate to the morphology and the growth rate of Spirulina platensis population. This research method is completely randomized design with three treatments and three replications. The period of treatment started in the exponential phase with carbon dioxide rate at 0.1 lpm, 0.2 lpm, and 0.4 lpm. The results showed that carbon dioxide is given can be used by S. platensis to stimulating the growth but this will also shorten the growth kinetics. This is also reflected in the results of the statistical analysis that there is no significant defference (p>0,05). And the morphological responses of S. platensis are fragmentation and lysis cell.ABSTRAK Pencemaran karbondioksida terjadi karena peningkatan penggunaan bahan bakar fosil untuk industri dan transportasi. Akibat terjadinya deforestri penyerapan karbondioksida oleh lingkungan terrestrial berkurang, sehingga terjadi peningkatan penyerapan karbondioksida oleh laut. Penyerapan karbondioksida oleh laut akan menyebabkan perubahan sifat kimia laut yang berdampak pada ekosistem laut. Spirulina platensis sebagai organisme kosmopolitan yang terdapat di laut dapat menggunakan karbon anorganik yang terserap dalam laut. Penelitian ini bertujuan untuk mempelajari efek laju karbondioksida terhadap morfologi dan laju pertumbuhan populasi Spirulina platensis. Metode penelitian ini adalah rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan. Masa perlakuan dimulai pada fase eksponensial dengan laju karbondioksida 0,1 lpm, 0,2 lpm, dan 0,4 lpm. Hasil penelitian menunjukkan bahwa karbondioksida yang diberikan mampu digunakan oleh S. platensis untuk meningkatkan pertumbuhan tetapi juga akan memperpendek kinetika pertumbuhan. Hal ini juga tercermin pada hasil analisis statistiknya yang tidak ada beda nyata (p>0,005). Secara morfologi respon S. platensis terhadap pemberian karbondioksida adalah terjadinya fragmentasi dan lisis sel.

Toxicity of Copper (Cu) and Chromium (Cr) on the Seed Germination of Mung Bean (Vigna radiata L.) Sutan Nur Chamida Tri Astuti; Andhika Puspito Nugroho
Journal of Biotechnology and Natural Science Vol. 2 No. 2 (2022)
Publisher : Universitas Ahmad Dahlan

Rapid industrial growth may have implications for an increase in cases of pollution of soil ecosystems through untreated waste disposal. Copper and chromium are metals found in some types of industrial wastewater, such as electroplating and silversmithing, that are essential at low concentrations but toxic at high concentrations. Vigna radiata was chosen as the test organism because it has been reported that the yield and production of the crop have decreased worldwide due to heavy metal pollution. This study aims to evaluate individual and mixed Cu and Cr toxicity in the seed germination of V. radiata. The individual and mixed Cu and Cr toxicity test was started with a range-finding and definitive test for 96 h. The toxicity level of the individual metal was expressed as IC50-96 h by probit analysis. For the mixed test, the organism was exposed to concentration ratios of Cu and Cr: 10%:90%; 35%:65%; 50%:50%; 65%:35%; and 90%:10% of individual IC50-96 h, each conducted for 96 h. The results showed that the individual IC50-96 h of Cu was 127.4 mg/L, while for Cr, it was 615.23 mg/L, indicating that Cu is more toxic than Cr. The mixed test showed that the IC50-96 h of Cu was 247.5 mg/L, 579.85 mg/L for Cr. The highest inhibition value at the mixed test was at a Cu and Cr concentration ratio of 90%:10%. Based on the mixed test, the interaction of Cu and Cr resulted in an additive effect (CI=1).

Literature Review and Experiment: Diversity of Bacteria in Forest, Revegetated Post-Mining Land, and Active Tin Mining with A Metagenomic Approach Eka Sari; Andhika Puspito Nugroho; Endah Retnaningrum; Irfan Dwidya Prijambada
Indonesian Journal of Science and Technology Vol 8, No 1 (2023): IJOST: April 2023
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v8i1.51662

The objective of this study was to investigate the diversity of bacteria in the rhizosphere of Acacia grow in the forest and different ages of revegetated land and non-rhizospheric bacteria from active tin mining soil on Bangka Island with a metagenomic approach and to investigate its relationship with physicochemical properties of the soil and its metal content. 17 species/genera were found in the four fields, and Bradyrhizobium is the dominant genus. Bacterial communities of the forest (more than 5 years in revegetated post-tin mining land containing C, N, P, and Fe) are Paraburkholderia sp., Bacillus sp., Candidatus-Xiphinematobacter, and Bacterium enrichment. The physicochemical properties of soil from less than 5 years of revegetated contain Cu, Cr, and Mo, and the dominant species in this soil were Bulkhorderia sp., Bacillus lehensis, B. fordii, B. thermolactis, Chloroflexi bacterium, and Thermonosporaceae bacterium. The physicochemical properties of soil from the mining were dominated by Cd and Ni, and the dominant species in this soil were Sinomonas atrocyanea.

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