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All Journal Teknosains: Media Informasi Sains dan Teknologi
Inez Johanna
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Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Physics

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DETECTION OF QUORUM QUENCHING -PRODUCING BACTERIA FROM RHIZOSPHERE SOIL AS A BIOCONTROL AGENTS Stella Magdalena; Inez Johanna; Yogiara Yogiara
Teknosains Vol 15 No 1 (2021): Januari-April
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v15i1.16178

Abstract

Quorum sensing is known as a communication mechanism among bacteria to control gene expression such as bioluminescence, pigmentation, and pathogenicity. Quorum quenching is known as inhibition of quorum sensing activity. In order to block quorum sensing activity, some bacteria produced enzymes which could degrade AHL, such as AHL-acylase, AHL-lactonase, and AHL-oxidase and reductase. In this study, soil bacteria were isolated and screened for their quorum quenching activity. These isolates were divided into Streptomyces and non-Streptomyces isolates. Detection were done by using Chromobacterium violaceum as an indicator bacteria for pigment inhibition by quorum quenching activity. About 10 out of 695 isolates were shown to possess quorum quenching activity. These isolates were further identified by biochemical tests, Gram staining, and identified by 16S rRNA sequencing. Four positive isolates showed similarity with Streptomyces sp., and 6 positive isolates were non-Streptomyces which showed similarity with Pseudomonas putida, Bacillus pumilus, Bacillus sp., Enterobacter ludwigii, and Enterobacter sp.
Co-Authors Stella Magdalena Yogiara Yogiara