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All Journal Teknosains: Media Informasi Sains dan Teknologi Jurnal Fisika dan Terapannya
Sri Zelviani
UIN Alauddin Makassar
Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Physics

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ANALISIS NILAI ABSORBANSI KADAR FLAVONOID TANAMAN HERBAL MENGGUNAKAN SPEKTROFOTOMETER UV-VIS Anita Purnamasari; Sri Zelviani; Sahara Sahara; Nurul Fuadi
Teknosains Vol 16 No 1 (2022): Januari-April
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v16i1.24185

Abstract

Flavonoid merupakan metabolit sekunder dari polifenol yang banyak ditemukan pada tumbuhan dan makanan serta memiliki aktivitas biologis. Kadar flavonoid dapat diukur dengan mengetahui nilai absorbansi pada panjang gelombang tertentu berdasarkan prinsip Lambert-Beer dari setiap tanaman herbal menggunakan spektrofotometer UV-Vis. Tujuan penelitian ini adalah untuk mengetahui kadar flavonoid ekstrak daun sirih merah, daun kembang sepatu dan ekstrak daun kapuk. Proses ekstraksi kandungan kimia dan daun sirih daun waru dan daun kapuk dilakukan dengan metode maserasi menggunakan etanol 70%. Hasil peneltian menunjukkan bahwa kandungan flavonoid total daun sirih merah, daun kembang sepatu dan daun kapuk adalah sebesar 1,18131%, 1,4445% dan 1,2985%. Sehingga dapat disimpulkan bahwa kandungan flavonoid tertinggi dimiliki oleh daun sirih merah dengan nilai sebesar 1,8131%.
ANALISIS NILAI ABSORBANSI UNTUK MENENTUKAN KADAR FLAVONOID DAUN JARAK MERAH (JATROPHA GOSSYPIFOLIA L.) MENGGUNAKAN SPEKTROFOTOMETER UV-VIS Ahriani Ahriani; Sri Zelviani; Hernawati Hernawati; Fitriyanti Fitriyanti
JFT : Jurnal Fisika dan Terapannya Vol 8 No 2 (2021): DESEMBER
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jft.v8i2.23379

Abstract

Research on Absorbance Value Analysis on Determination of Flavonoid Levels in Red Jatropha Leaves (Jatropha Gossypifolia L.) using UV-Vis Spectrophotometer has been conducted. This study aimed to determine the absorbance of red jatropha (Jatropha Gossypifolia L.) leaves on young and old leaves and to determine the flavonoid content of red jatropha (Jatropha Gossypifolia L.) leaves on young and old leaves. The sample used in this study was 200 g of red jatropha leaf powder (Jatropha Gossypifolia L.) with the addition of 2000 ml of 70% ethanol as solvent in a ratio of 1:10. The extraction method used in this study is the MAE (Microwave Assisted Extraction) method to measure the absorbance value of the sample at a wavelength of 436 nm using a UV-Vis spectrophotometer. The results showed that the absorbance value of red jatropha (Jatropha Gossypifolia L.) leaves on young leaves was smaller than on old leaves. Where the absorbance value in young leaves is 0.355 while the absorbance value in old leaves is 0.616. The resulting absorbance value has complied with Lambert-Beer's law (0.2 ≤ A < 0.8). The flavonoid content of red jatropha (Jatropha Gossypifolia L.) leaves in young leaves was lower than in old leaves. Where the levels of flavonoids in young leaves is 2.71% while the levels of flavonoids in old leaves is 4.90%. This shows that the greater the absorbance value, the greater the flavonoid content produced.
Co-Authors Ahriani Ahriani Anita Purnamasari FITRIYANTI FITRIYANTI HERNAWATI HERNAWATI Nurul Fuadi Sahara Sahara