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Production Of Malto-Oligosaccharides From Cassava Cultivar Kuning Nanik Rahmani; Ade Andriani; nFN Yopi; Sri Hartati
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 3 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v12n3.2015.147-155

Abstract

Characteristic the physic-chemical of Indonesia cassava starch from four cultivated varieties has been conducted for maltooligosaccharide production. Result of proximate analysis of the extracted starch indicated that the extracted starch was quite pure. The purity of the extracted starch was visually confirmed by microscopic analysis by using SEM micrographs at 2500X magnifications show that the integrity of the granules starch as intact. Based on the amylopectin and amylase content showed that one of cultivated variety of cassava, cultivated variety Kuning contain the amylopectin higher than amylase was compared with the other cultivated variety. The next focus research was analysis potential of starch from cultivated variety Kuning for maltooligosaccharide production by enzymatic hydrolysis by ?-amylase from marine bacterium Brevibacterium sp. The optimum hydrolysis condition for cultivated variety Kuning was obtained substrate concentration 4.5% (b/v), comparison of substrate: enzyme 1:2, temperature reaction 30oC with reducing sugars concentration of 13.359 ppm. The hydrolysis products of cassava starch cultivated variety Kuning were maltooligosaccharides mixture, yielding maltose, maltotriose, maltotetraose, maltopentaose. This result showed that cassava starch of cultivated varieties Kuning potential for maltooligosaccharides production. PRODUKSI MALTOOLIGOSAKARIDA DARI UBI KAYU VARIETAS KUNINGKarakteristik fisiko kimia karbohidrat dari empat varietas kultivar asal Indonesia dilakukan untuk melihat potensinya sebagai bahan baku untuk produksi maltooligosakarida. Analisa proksimat karbohidrat hasil ekstraksi dari keempat varietas kultivar ubi kayu mengindikasikan bahwa karbohidrat yang dihasilkan cukup murni. Kemurnian dari karbohidrat tersebut terlihat setelah dikonfirmasi dengan analisa mikrokospis dengan menggunakan mikroskop electron SEM dengan pembesaran 2500X yang menunjukkan bahwa granulanya utuh. Berdasarkan kadar amilopektin dan amilosa menunjukkan bahwa salah satu varietas kultivar ubi kayu yaitu varietas Kuning mengandung amilopektin lebih tinggi dibandingkan kadar amilosanya jika dibandingkan dengan tiga varietas kultivar lainnya. Fokus penelitian selanjutnya adalah analisa potensi karbohidrat varietas kultivar Kuning tersebut untuk produksi maltooligosakarida dengan hidrolisis enzimatis oleh ?-amylase dari Brevibacterium sp. Kondisi optimum hidrolisis dari varietas kultivar Kuning diperoleh pada konsentrasi substrat 4.5% (b/v), perbandingan substrat dan enzim 1:2, suhu reaksi 30oC dengan kadar gula reduksi yang diperoleh 13.359 ppm. Produk hidrolisis dari ubi kayu varietas kultivar Kuning adalah berupa campuran maltooligosakarida yang terdiri atas maltose, maltotriose, maltotetraose, maltopentaose. Hasil ini menunjukkan bahwa karbohidrat ubi kayu dari varietas kultivar Kuning mempunyai potensi untuk digunakan sebagai bahan baku untuk produksi maltooligosakarida.
PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI Aspergillus ustus BL5 Yopi Yopi; Nanik Rahmani; Ade Andriani; Fitria Dewi; Anja Meryandini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
PENINGKATAN KEMAMPUAN PEMECAHAN MASALAH MATEMATIK MAHASISWA FMIPA PENDIDIKAN MATEMATIKA MELALUI MODEL PEMBELAJARAN IMPROVE Ade Andriani
JURNAL TARBIYAH Vol 23, No 1 (2016)
Publisher : UIN Sumatera Utara Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1121.927 KB) | DOI: 10.30829/tar.v23i1.107

Abstract

The purpose of research in the design of this experiment was to investigate the apparent increase in the mathematical problem solving abilities of students. The research was conducted in the Department of Mathematics Education, State University of Medan. This research is an experimental study with the study design pre-test-post-test control group design. The population in this study were all students of semesters 1 (one) UNIMED mathematics education majors to take samples of two classes (class experimental and control classes) through random sampling technique. Data obtained from tests the ability of solving mathematical problems. Data were analyzed by ANOVA two lanes. Before use ANOVA two-lane first tested for normality and homogeneity in research in this study with a significant level of 5%. The results showed that the average increase problem solving ability test experimental class was 0.48 and 0.38 in the control class is sig = 0.02 with 0.02 <α = 0.05 then there is an increase in mathematical problem solving ability students are taught by learning models IMPROVE higher than in the Direct Learning. The findings of the study recommend IMPROVE be one learning approach used in primary school or university to achieve a high level of competence thinking.Keywords    : Improve Learning Model, Mathematical Problem Solving Ability