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All Journal Forum Pasca Sarjana Jurnal Veteriner
Aisjah Girindra
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Education Veterinary

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Isolation and Identification of Serum Gamma Immunoglobulin (IgG) of Native and Imported Chickens By Ion Exchange Chromatography and Immunochernistry Methods Murniaty Simorangkir; Aisjah Girindra; Fachriyan H. Pasaribu; Wasmen Manalu
Forum Pasca Sarjana Vol. 18 No. 1 (1995): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

The study was designed to isolate and identify serum IgG of native and imported chickens, after being immunized with Newcastle Disease Vaccine. The isolation method used was the DEAE-Cellulose ion exchange chromatography using 0.01 M Tris-HCl buffer, at pH 8.0, with linear gradient NaCl from 0.01 M to 0.30 M after salting out with anhydrous Na2SO4. Identification of IgG characteristics carried out using the Ouchterlony double immunodiffusion, immuno-electrophoresis and SDS-PAGE 8% methods. Serum fractionation of native and imported chickens using the DEAE-Cellulose chromatography, after salting out with anhydrous Na2So4 of 18,14 and 14% resulted in four peaks of protein fractions.
Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS) Iwan Harjono Utama; Aisjah Girindra; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan; Endhie D. Setiawan; Gatut Ashadi; Aida Louise Tenden Rompis
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS)
Co-Authors Aida Lousie Tenden Rompis Endhie D. Setiawan Fachriyan H. Pasaribu Fachriyan Hasmi Pasaribu Gatut Ashadi I wayan Teguh Wibawan Iwan Harjono Utama Murniaty Simorangkir Wasmen Manalu