The purpose of this research is to know whether the aqueous extract of purple nutsedge(Cyperus rotundus) have an effect on the growth and chlorophyll content of upland riceseedling of Inpago 8 varieties. This research was conducted at Botanical Laboratory ofBiology Department Faculty of Mathematics and the Natural Sciences University ofLampung from September to October 2017. The experiments were carried out in a completerandomized design with the main factors being aqueous extract of purple nutsedge with 5 level : 0% v / v (control), 5% v / v, 10% v / v, 15% v / v, and 20%v/v. Parameters measuredwere shoot length, fresh weight, dry weight, relative water content, and total chlorophyllcontent of rice seedling. Homogeneity of variance was determined by the Levene test at 5%significant level. Analysis of variance and Tukey HSD test is done at 5% significant level.The results showed that the concentration of extract correlated quadratic with shoot lengthwhere the maximum shoot length was 3.9 cm at 10% extract concentration. Fresh weight andrelative water content maximum were 73.21 mg and 76% respectively at concentrations of14% and 10%. The minimum total chlorophyll content was 0.47 mg / g tissue at aconcentration of 11%. There was no significant effect on the dry weight of the seedling.From the results of the study, it was concluded that the extract of purple nutsedge at aconcentration below 14% was a growth stimulator, but it was an inhibitor of chlorophyllbiosynthesis.Keyword: Chlorophyll, Growth, Upland rice variety Inpago 8, Purple nutsedge