E. Handiwirawan
Indonesian Center for Animal Research and Development, Jl. Raya Pajajaran Kav E-59, Bogor 16151

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Journal : Journal of the Indonesian Tropical Animal Agriculture

Polymorphism of 5’UTR myostatin gene indel (g.1256/TTTTA) and its association with body weight in Boerka crossbred goat Ismail, R.; Handiwirawan, E.; Elieser, S.; Jakaria, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.163-172

Abstract

This study aimed to identify the variation of 5’UTR Myostatin (MSTN) gene and its association to body weight in Boer, Kacang, and Boerka goats. DNA samples were obtained from 149 heads of the goats from the Indonesian Goat Research Center, Sungei Putih, North Sumatera. Polymorphism identification was conducted by direct sequencing and PCR-RFLP with DraI as the restriction enzyme for indel g.1256/TTTTA. Analysis of variance for body weight was carried out using the General Linear Model (GLM). The 5’UTR MSTN gene|DraI was monomorphic in Kacang but polymorphic in Boer and Boerka. Genotype frequencies for Boer 0.40(AA), 0.43(AB), 0.17(BB); Kacang 1.00(AA); Boerka 0.53(AA), 0.37(AB), 0.10(BB). The allele frequencies for Boer 0.62(A), 0.38(B); Kacang 1.00(A); Boerka 0.72(A), 0.28(B), respectively. AB was the most frequent genotype among Boer, but AA was the most frequent in Kacang and Boerka. Indel g.1256/TTTTA has a significant effect (P<0.05) only on birth weight (BW) of Boer, but no significant effect on other bodyweight parameters both in Boer and Boerka.AA genotype has the highest BW (P<0.05) than AB, but it’s not significantly different from BB. Indel g.1256/TTTTA could be used as a genetic marker for the birth weight of Boer but not in Boerka goats. 
THE DIFFERENTIATION OF SHEEP BREED BASED ON THE BODY MEASUREMENTS Handiwirawan, E.; Noor, R.R.; Sumantri, C.; Subandriyo, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 1 (2011): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.1.1-8

Abstract

This research was carried out to make a difference and to predict genetic distance some sheep thatare genetically related to each other based on the body size measurements. Nineteen bodies size of 323sheeps of five sheep breeds; namely Barbados Black Belly Cross (BC), Garut Local (GL), GarutComposite (GC), Sumatra Composite (SC) and St. Croix Cross (SCC) were measured. Analysis ofvariance and canonical discriminant analysis, Mahalanobis distance, plotting canonical and dendogramwere performed using PROC GLM, PROC CANDISC, PROC CLUSTER and PROC TREE of SASsoftware ver. 9.0. Index Length, Width Slope, Depth Slope, Balance and Cumulative Index of GC sheepwere significantly higher than the four other breeds. Canonical discriminant analysis successfully coulddifferentiate among the five sheep breeds. All five breed were divided into two groups: the first groupconsisted of SCC, SC and BC; and the second group consisted of the GL and GC. The results of geneticdistance estimation showed that the SCC had a value of sheep genetic distance closest to SC (10.83) andBC (27.98), while GL had the closest distance to GC (66.60). The tail width, horn base circumference,horn length (canonical 1) and variable length of the tail and body length (canonical 2) were the breeddifferentiation variable in this study.