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All Journal Jurnal Agripet Jurnal Sain Peternakan Indonesia
Yosi Fenita
Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Veterinary

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Journal : Jurnal Sain Peternakan Indonesia

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Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods Teguh Rafian; Yurnalis Yurnalis; Yosi Fenita; Rafi Iskandarsyah
Jurnal Sain Peternakan Indonesia Vol 17 No 3 (2022)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.17.3.170-174

Abstract

This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.
Co-Authors Ahmat Kusnandar Nurmeiliasari Nurmeiliasari Rafi Iskandarsyah Teguh Rafian Yurnalis Yurnalis