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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal SOLMA Journal of Halal Science and Research Jurnal Natural
HANIFAH RAHMI
Universitas Muhammadiyah Prof. DR. HAMKA
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Other

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Construction of recombinant sox2-encoding plasmids that regulate pluripotency of breast cancer stem cells from indonesian patient Hanifah Rahmi; Tiodinar Theresia; Amarila Malik; Septelia Inawati W
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.1 KB) | DOI: 10.12928/pharmaciana.v9i1.11919

Abstract

A therapy development with recombinant protein is a new and potential innovation to destroy cancer stem cells (CSCs). Various ways of killing CSCs include the provision of polyvalent anti-protein antibodies that code pluripotency. Therefore, it takes a mixture of Oct-4, c-Myc, Sox2, and Klf4 protein antigens that can stimulate the formation of polyvalent antibodies. This study aimed to construct Sox2 recombinant by identifying the target genes by reverse transcriptase PCR and then arranging their designs to be inserted into the cloning vector pET101/D-TOPO®. The target gene was developed by finding the complete sequences of Sox2 nucleotides on the NCBI GenBank. The growth on LB-ampicillin agar plates was amplified by PCR to obtain colonies with pET101/D-TOPO® vectors and inserts of the pluripotent gene of CSCs, then the PCR results were observed through electrophoresis. A total of fifteen colonies have DNA bands with a base pair of about 300 bp in length. The recombinant clones produced Sox2 genes with a base length of 330 bp.
Workshop dan Pelatihan Pengajuan Sertifikat Halal bagi Pelaku Industri Makanan Olahan UMKM Siska Siska; Hanifah Rahmi; Fitriani; Ema Dewanti
Jurnal SOLMA Vol. 9 No. 1 (2020)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.194 KB) | DOI: 10.29405/solma.v9i1.3823

Abstract

Indonesia adalah negara yang memiliki warga negara mayoritas adalah umat muslim. Sebagai umat Islam, kita diwajibkan mengkonsumsi makanan/minuman yang halal. Seiring dengan hal tersebut Pemerintah telah mengeluarkan Undang-Undang Nomor 33 Tahun 2014 tentang Jaminan Produk Halal, maka semua pelaku industri baik di bidang makanan/minuman olahan, produk farmasi dan lain-lain wajib memiliki sertifikat halal. Namun hingga saat ini belum banyak indutri olahan yang mengajukan sertifikat halal terutama pada industri UMKM (usaha mikro, kecil dan menengah). Fakultas Farmasi dan sains (FFS) UHAMKA yang telah memiliki Pusat Kajian Halal UHAMKA (PKHU), berkewajiban untuk mensosialisasi dan mengedukasi kepada seluruh masyarakat mengenai pentingnya “halal” suatu produk. Target pada kegiatan workshop ini adalah pelaku usaha di bidang pengolahan makanan/minuman UMKM di wilayah Duren Sawit Jakarta Timur. Metode yang digunakan adalah dengan melakukan pelatihan/workshop tentang tatacara pengajuan sertifikat halal dan dokumen-dokumen yang harus dipersiapkan. Workshop diawali dengan sosialisasi pentingnya sertifikat halal kemudian dilanjutkan dengan pretest. Pre-test untuk melihat sejauh mana pemahaman terkait sertifikasi halal. Workshop diakhiri dengan post-test, untuk melihat peningkatan pemahaman setelah diberikan sosislaisasi dan pelatihan terkait sertifikasi halal. Hasil yang didapat adanya peningkatan pemahaman peserta (p<0,05) dibandingkan antara sebelum dengan sesudah pelatihan. Kesimpulan dari kegiatan ini adalah workshop yang diberikan bermanfaat untuk para pelaku usaha UMKM dalam meningkatkan pemahaman terkait tatacara sertifikasi halal.
Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method HARIYANTI HARIYANTI; HANIFAH RAHMI
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (990.482 KB) | DOI: 10.24815/jn.v21i3.18874

Abstract

Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}
Narrative review: a study of pork DNA analysis methods in food gelatin Nisa Aulia Ansor; Hariyanti Hariyanti; Hanifah Rahmi
Journal of Halal Science and Research Vol. 4 No. 2 (2023): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v4i2.7798

Abstract

Gelatin is a substance resulting from the partial hydrolysis of collagen consisting of animal cartilage, skin, and bones from water-soluble polypeptides. The problem formulation in this study is what DNA analysis methods can be used to detect pig DNA in food gelatin. This study aimed to collect, review, and conclude information regarding the most widely used DNA analysis method to detect pig DNA in food gelatin. From the results of the literature study, it can be concluded that the most widely used extraction method in the analysis of porcine DNA in food gelatin is the DNeasy® Mericon Food kit extraction method for good purity results, there is the foodproof® III Kit method, the universal Wizard KIT Promega®, the DNeasy® Mericon Food kit, and the Column based FavorPrep™ Food DNA Extraction Kit. The pig DNA analysis method is widely used in conventional PCR. Sensitivity, fast testing time, and gh acporcine gelatin sample was identified by the appearance of a band on the electrophoresis results. Keywords: Food Gelatin, Extraction Method, DNA Analysis, Journal Review.
Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method HARIYANTI HARIYANTI; HANIFAH RAHMI
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i3.18874

Abstract

Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}
Co-Authors Amarila Malik Ema Dewanti Fitriani Hariyanti Hariyanti Nisa Aulia Ansor Septelia Inawati W Siska Siska Tiodinar Theresia