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Koichi Fujie
Department of Ecological Engineering, Toyohashi University of Technology, Toyohashi
Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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Rapid Determination of Microbial Quinones using Supercritical CO2 Extraction Muhammad Faisal; Irvan Irvan; Yoichi Atsuta; Hiroyuki Daimon; Koichi Fujie
Jurnal Rekayasa Kimia & Lingkungan Vol 5, No 1 (2006): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

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Abstract

The supercritical CO2 extraction of microbial quinones from activated sludge samples obtained from various activated sludge was investigated and compared to the conventional method using organic solvent extraction. The extraction was carried out in a supercritical fluid extraction (SFE) system in the temperature range of 25 to75 oC and the pressure up to 30 MPa. Different extraction conditions, such as the temperature, pressure, extraction time and modifier were employed to maximize the SFE efficiency. Significant amount of microbial quinones (ubiquinones and menaquinones) could be extracted rapidly with supercritical CO2. Results on the value of diversity and dissimilarity suggested that the SFE with supercritical CO2 extraction was a reliable technique for quinones extraction.
Co-Authors Hiroyuki Daimon Irvan Irvan Muhammad Faisal Yoichi Atsuta