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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter PERTANIAN TROPIK
Luthfi A. M. Siregar
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Agriculture, Biological Sciences & Forestry Engineering Other

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Analysis of Centelloside of Pegagan (Centella asiatica) Noverita S. Vinolina; Narendra Singh; Justin A. Napitupulu; Luthfi A. M. Siregar; Marline Nainggolan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.39 KB)

Abstract

Centella asiatica is still categorized as a wild plant that has not undergone domestication. Sustainability of medicinal and aromatic plants for sustainable harvesting of medicinal plants so that it is not only in nature but also the harvest of cultivated so obtained uniform quality. Chemical constituents arealready known: contain some saponin compounds, including asiaticoside, madeccasoside and asiatic acid. This study aims to determine the bioactive content; asiaticoside, madeccasoside and asiatic acid on the top(leaves and petiol) and bottom (roots and tendrils) with HPLC method. The experiment was conducted in growth chamber Department of Biological Sciences and School of Pharmacy Auburn University, USA. This study conducted in September-December 2011. The materials used are Centella asiatica of Deli Serdang accession, Kabanjahe accession, Berastagi accession and Samosir accession. Equipment needed to support the growth chamber study were digital scales, plastic pots, HPLC system types waters alliance 2695 autosampler, 996 photodiode array detector, coulomb adsorbosphere C18 5μ, size 250 x 4.60 mm, UV absorbance, wavelength of 210 nm, flow rate 1.8 ml / min, injection volume of 20 mL, Empower Pro software, and others. The results that the pattern of centelloside (asiaticoside, madecassoside and asiatic acid), when the content of one of the high content of bioactive, the others bioactive will be lower or biosynthetic pattern toward a compound needed. Centelloside plant age affects the content of Centella asiatica. Centelloside pattern is influenced by the condition of the planting medium, very high levels of phosphorus, biosynthesis of centelloside more toward to asiaticoside.
In vitro The Role of Paclobutrazol in Potato Seed Production (Solanum tuberosum L) cultivar Granola Kembang Early Generation (G0) In Vitro: Potato Hans Samuel Purba; Hot Setiado; Luthfi A. M. Siregar
Jurnal Pertanian Tropik Vol. 8 No. 1 (2021): JURNAL PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (697.546 KB) | DOI: 10.32734/jpt.v8i1.5891

Abstract

The aim of the research was to determine the best paclobutrazol treatment to obtain superior quality seed potato cultivars. The research was conducted at the Tissue Culture Laboratory of Horticulture Technical Centre Service Unit, Gedung Johor, Medan, North Sumatra, Indonesia from January 2020 to March 2020. The complete randomized design with a single factor, 4 treatments, and 6 replications was used in this research. The results showed that the concentration of paclobutrazol affected the number of leaves, the shoots length, the number of shots, the number of nodes, the number of internodes, the stem diameter, and the percentage of roots formed but not significantly affected the number of roots. The M2 treatment, MS + 9 ppm paclobutrazol, showed the best treatment with an average plantlet performance value of 7.33 and the plantlets showed the compact morphology with larger stem diameter and smaller thick dark green leaves.
Co-Authors Hans Samuel Purba Hot Setiado Justin A. Napitupulu Marline Nainggolan Narendra Singh Noverita Sprinse Vinolina