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All Journal Nexus Biomedika Nexus Kedokteran Translasional
Riza Novierta Pesik
Sebelas Maret University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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The Effect of Propolis Ethanolic Extract on Serum Malondialdehyde level of mice Balb/c induced by cigarette smoke Riyan Angga Putra; Diding Heri Prasetyo; Riza Novierta Pesik
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Propolis' ethanolic extract contains many kind of potent antioxidant substances, such as resveratrol, chrysin and caffeic acid in abundant quantities. These cause Propolis owns prosperity to be antioxidant and antiinflamation agent which are able to fight against free radical effect of cigarette smoke, thus lipid peroxidation won't exist and the Malondialdehyde (MDA) formation can be banned. The objective of this research was to prove the effect of Propolis' ethanolic extract on serum MDA level of mice balb/c which were induced by cigarette smoke. Methods: This was a laboratory experimental research the post test only control group design. Samples were twenty five mice, Swiss webster type (age 6-8 weeks; weight 20-30 gram) divided into 5 groups, each group had five mice. Mice for Control Group (KK) and Induction Group (KI) were administered by distilled water. While, the First Treatment Group (KP1), the Second Treatment Group (KP2) and the Third Treatment Group (KP3) were administered Propolis' ethanol extract by dose 5.6 mg, 11.2 mg and 16.8 mg per 20 g of body weight of mice respectively. All groups except KK, were induced by smoke of one cigarette stick per day before extract administration. This research was done for 14 days. On day 15th, all mice were sucked the blood, then the MDA level were measured. The data were analyzed by using One-Way ANOVA (? = 0.05) and continued by Post Hoc test (? = 0.05). Results: The results of One-Way ANOVA test showed that there was significant difference among four-groups. Post Hoc test results showed there was significant difference among all groups. Conclusion: The administration of Propolis' ethanolic extract of dose 5.6 mg/20 g of body weight of mice can decrease the serum malondialdehyde level of mice Balb/c which are induced by cigarette smoke significantly. Keywords: Propolis, Cigarette smoke, Malondialdehid level
Molecular Docking Analysis of Liliaceae FamilyPhytochemicals to Estrogen Receptor ? on Breast Cancer Compared to Genistein Bryan Pandu Permana; Riza Novierta Pesik; Fikar Arsyad Hakim
Nexus Kedokteran Translasional Vol 4, No 2 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Breast cancer is the second highest cancer incidence in the world with 1.7 million women were diagnosed in 2012. Interaction between estrogen and estrogen receptor ? (ER?) have an important role in cancer progressivity and resistancy to chemotherapy. Genistein is one of the phytoestrogen that has selective estrogen receptor modulator (SERM) activity so it can inhibit the activity of estrogen receptor ? (ER?) on the breast cancer. Other phytochemicals are expected to have more affinity to ER? than genistein. Anticancer activity of Liliaceae family phytochemicals have been found but its inhibition to ER? is unknown. This research analyzes the interaction between Liliaceae phytochemicals to ER? by molecular docking technique. Method: This research is bioinformatics observational research that observed the interaction between Liliaceae phytochemicals to ER?. The molecular docking analysis was performed using PLANTS 1.1, preparation program MarvinSkecth 5.2 and YASARA 10.1. Visualization of the molecular docking was performed using PyMOL 1.3. Results: Six substances have lower docking score than genistein (-79.21). Those are spiraeoside (-84.80), stigmasterol (-84.28), cyanin (-83.79), beta-sitosterol (-82.81), progesterone (-82.68) and idaein (-82.22). Spiraeoside, cyanin, and idaein have one or more hydrogen bond to ER? in visualization. The six substances bind ER? on four same amino acids that bindgenistein, those are Trp383, Leu525, Met528, and Cys530. Conclusio: Molecular docking analysis of Liliaceae phytochemicals found six substances that have higher affinity to ER? than genistein with three substances have hydrogen bond and similar structure to the genistein. Keyword: Breast Cancer, Estrogen Receptor ?, Genistein, Liliaceae Family Phytochemical, Molecular Docking.
The Effect of Propolis Ethanolic Extract on the degree of lung damage of mice (Mus musculus) which induced by cigarette smoke . ismael; Diding Hery Prasetyo; Riza Novierta Pesik
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: The Propolis ethanolic extract contains a variety of potent antioxidants, especially flavonoids and Caffeic Acid Phenylethyl Ester (CAPE). Flavonoids have a very strong antioxidant effect against free radicals capable of cigarettes. Meanwhile, CAPE is a potent inhibitor of Nuclear Factor kappa-light-chain-enhancer of activated B cell (NF-kB). Nuclear Factor kappa-light-chain-enhancer of activated B cell (NF-kB) is an essential mediator of inflammation. This study aims to demonstrate the effect of ethanolic extract of propolis can reduce the degree of lung damage of mice induced by cigarette smoke. Methods: This was a laboratory experimental research the posttest only control group design. Samples were twenty five mices Swiss Webster type (age 2-3 months; weight 20-30 gram) divided into 5 groups, each group has five mices. Mices for Control Group (KK) and Induction Group (KI) were administered by aquadest. While, the first Treatment Group (KP1), the second Treatment Group (KP2) and the third Treatment Group (KP3) were administered propolis ethanolic extract by dose 5.6 mg/20 g weight of mice, 11.2 mg/20 g weight of mice, and 16.8 mg/20 g weight of mice respectively. All groups except KK, were induced by smoke of one cigarette stick per day before extract administration. This research was done for 14 days. On day 15th, the mice were killed and lung organ was isolated and prepared histologically using paraffin method and then stained with haematoxylin and eosin (HE). The Data were analyzed by using Kruskall Wallis (? = 0.05) and continued by Mann Whitney (? = 0,05). Results: The results of Kruskall Wallis test showed that there was significant difference between five-groups. Mann Whitney test results showed there was significant difference between grups of KK-KI, KI-KP1, KI-KP2,KI-KP3, KP1-KP2,KP1-KP3 and KP2-KP3. Conclusion: Propolis ethanolic extract decreasethe degree of lung damage of mice (Mus musculus) which induced by cigarette smoke. Keywords: Propolis ,cigarette smoke, degree of lung damage, mice.
Co-Authors . ismael Bryan Pandu Permana Diding Heri Prasetyo Diding Hery Prasetyo Fikar Arsyad Hakim Riyan Angga Putra