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All Journal Current Biochemistry Jurnal Kompetitif BioEksakta : Jurnal Ilmiah Biologi Unsoed
Dodi Safari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Immunology & microbiology Veterinary

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Activity of Skin Secretions of Frog Fejervarya limnocharis and Limnonectes macrodon against Streptococcus pneumoniae Multidrug Resistant and Molecular Analysis of Species F. limnocharis Jajang Suhyana; I Made Artika; Dodi Safari
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Indonesia has about 450 frog species which is approximately 20% of frog species in the world. Among frog species found in Indonesia are Fejervarya limnocharis dan Limnonectes macrodon belonging to family Dicroglossidae. Frog skin secretion is considered to have a potency to be used as an alternative source of antibacterial agent against Streptococcus pneumoniae multidrug resistant (MDR). The aims of the present study were to analyze antibacterial activity of skin secretions of F. limnocharis and L. macrodon against S. pneumoniae multidrug resistant (MDR) and conduct molecular phylogenetic analysis of the frog used to ensure classification of frog species. The release of skin secretion was stimulated using epinephrine injection. Antibacterial activity of the skin secretions was tested using the well and paper disc methods. Results showed that skin secretions of F. limnocharis have antibacterial activity against S. pneumoniae multidrug resistant (MDR) SPN1307. The activity, however, was lower compared to that of chloramphenicol in both well and paper disc methods. On the other hand, skin secretions of L. macrodon failed to inhibit the growth of S. pneumoniae multidrug resistant (MDR) SPN1307. Molecular phylogenetic analysis was carried out on F. limnocharis based on DNA sequence of a partial fragment of mitochondrial cytochrome oxidase subunit I (COI) gene. Results showed that the frog F. limnocharis is closely related (97%) to Fejervarya sp from Bali. Skin secretions of F. limnocharis, therefore, has the potency to be developed as a source of antibacterial agents against S. pneumoniae multidrug resistant (MDR) SPN1307.
ANALISIS SUMBER DAN PENGGUNAAN MODAL KERJA PADA PT. ARITA PRIMA INDONESIA Tbk TAHUN 2016-2019 Dodi Safari; Eka Sevtia Mesta
JURNAL KOMPETITIF Vol 9, No 2: Edisi Juli-Desember 2020
Publisher : Fakultas Ekonomi Universitas Tridinanti Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52333/kompetitif.v9i2.713

Abstract

This study aims to see the working capital conditions of PT. Arita Prima Indonesia Tbk by calculating the source and use of working capital. The results of calculating the source and use of working capital at PT. Arita Prima Indonesia Tbk for the period 2016-2019, namely the development of working capital has increased by 3.39% in the 2016-2017 period and shows an excess of working capital in 2017 amounting to IDR 2,737,702,490. For the 2017-2018 period, the development of working capital increased by 28.52%, indicating an excess of working capital in 2018 amounting to Rp. 23,316,600,337. Whereas in the 2018-2019 period, the development of working capital decreased by 0.19%, indicating a shortage of working capital in 2019 amounting to Rp.204,079,037. The use of working capital at PT. Arita Prima Indonesia Tbk for the 2016-2019 period, namely the use of working capital in the 2016-2017 period is appropriate because in the operational management of PT. Arita Prima Indonesia Tbk has excess working capital of Rp. 2,737,702,490. The use of working capital in the 2017-2018 period is appropriate because in the operational management of PT. Arita Prima Indonesia Tbk, there is an excess of working capital of Rp. 23,316,600,337. Meanwhile, the use of working capital in the 2018-2019 period is not appropriate because in the operational management of PT. Arita Prima Indonesia Tbk, there is a working capital deficiency of Rp. 204,079,037. Keywords:Working Capital, Sources and Use of Working Capital
Identifikasi Serotipe dan Sensitivitas Antibiotik S. pneumoniae yang Dibawa Nasofaring Penderita Oma di Kabupaten Banyumas Miranti Oviani; Daniel Joko Wahyono; Dodi Safari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.118 KB) | DOI: 10.20884/1.bioe.2020.2.1.1779

Abstract

The aims of this study are to detect S. pneumoniae carried by nasopharnyx of children in primary school (aged 6-12 years) that diagnosed with AOM, to identify sensitivity of S. pneumoniae to antibiotics. The design of this study is nonexperimental survey with the descriptive analysis. Sampling was conducted in September - December 2018 in Banyumas district primary schools. Detection of S. pneumoniae was performed with microbiology methods. Meanwhile, serotype was determined by multiplex PCR and sensitivity to antibiotics was deduced using disc diffusion. The result of this study showed that carriage rate of S. pneumoniae carried by nasopharynx in children aged over 5 years that diagnosed with AOM in the Banyumas district was 35%. Serotype of S. pneumoniae obtainend from this study were 6A/6B, 6C/6D, 17F, 3, 13, 14, 23B, and untypeable. In addition, isolates of S. pneumoniae were highly susceptible to clindamycin (100%), erythromycin (100%), sulphametaxazole/trimethoprim (100%), chloramphenicol (88%), oxacillin (77%), and tetracycline (66%).
Co-Authors Daniel Joko Wahyono Eka Sevtia Mesta I MADE ARTIKA Jajang Suhyana Miranti Oviani