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All Journal Jurnal Ilmu Ternak dan Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
NLP Indi Dharmayanti
Balai Besar Penelitian Veteriner
Veterinary

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Amantadine resistance of clade 2.3.2 H5N1 Avian Influenza Virus from Waterfowl in Indonesia Dyah Ayu Hewajuli; NLP Indi Dharmayanti; I Wayan Teguh Wibawan
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i3.2764

Abstract

The objective of this research was to know the sensitivity of H5N1 clade 2.3.2 AIV from Indonesia to antiviral drug (amantadine) through molecular and in vitro tests. The study was conducted by virus isolation and identification, nucleotide analysis, and susceptibility to the amantadine hydrocloride in MDCK cells. The study result represented that the mean EID50 isolates of H5N1 clade 2.3.2 AIV was determined of >108 EID50/ml. The analysis of phylogenetic tree of M2 gene from six viruses of H5N1 clade 2.3.2 AIV from Indonesia were closed with H5N1 clade 2.3.2 AIV avian influenza viruses from Vietnam, China, Hongkong. The substitution of M2 protein (V27I) was identified in six isolates H5N1 clade 2.3.2 AIV isolated from Indonesia. Avian influenza of clade 2.3.2 H5N1 subtype from Indonesia produced the formation of CPE and the positive HA reaction with non-toxic concentration of amantadine hydrochloride in MDCK cells. The result of genetic analysis of M2 gene for amantadine resistance was related with the results of HA test and the formation of CPE in MDCK cells. These results established that amantadine resistance have been identified in H5N1 clade 2.3.2 AIV viruses isolated from Indonesia
Anticipation of the entry and spread of Equine Infectious Anemia in Horses in Indonesia Muharam Saepullah; Indrawati Sendow; Atik Ratnawati; NLP Indi Dharmayanti
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 29, No 1 (2019): March2019
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.089 KB) | DOI: 10.14334/wartazoa.v29i1.1917

Abstract

Horse is an attractive commodity in the world of horse matches, both for horse racing and equistrian. Recently, Asian countries including Indonesia have conducted international horse races. Horse traffic from several countries is unavoidable. This movement can lead to the entry of new diseases or the transmission of imported horses from local horses. At present, data on horse disease in Indonesia has not been widely reported, including Equine Infectious Anemia (EIA). This paper discusses EIA disease in horses about the etiology, characterization, spread of disease, its status in Indonesia and its treatment. EIA is a chronic disease characterized by high fever and thrombocytopenia. One of the factors that spread EIA infection, namely through traffic or horse movements. The highest risk factors for the spread of EIA disease are in the equestrian sector, followed by horse as a hobby, horse show or fattening horses for consumption. Wet environmental factors have higher risk to infection than dry areas. Applying the proper quarantine system, monitoring horse disease through surveillance, and implementing biosecurity at farms, location of events and at veterinary clinics, need to be done so that the entry of EIA disease can be anticipated as early as possible. This paper is expected to be useful and can be used as input for policy makers in the horse imports and movement.
Co-Authors Atik Ratnawati Dyah Ayu Hewajuli I wayan Teguh Wibawan Indrawati Sendow Muharam Saepullah