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Kusharto Kusharto
Jurusan Fisika Fakultas MIPA Universitas Brawijaya Jalan Veteran Malang 65145

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Insecticide Effects on Membrane Potential of Catfish Egg Cell (Clarias batrachus) Unggul Punjung Juswono; Kusharto Kusharto; Yeni Cahyati; Risalatul Latifah
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.853 KB) | DOI: 10.21776/ub.natural-b.2012.001.03.9

Abstract

Pollution has been occured in our environment due to daily life waste, industrial and using of peptiside in agroindustrial farm. Over usuage and dose of peptiside could be harmfull to the farm environment especially for fish farm. Measurement of membrane potential of fish egg cells is a simple metode to investigate water polution level. Membrane potential of fish egg cells can be measured using microelectrode probe which is connected to an electrometer. The changing of membrane potential value indicate the level of water polution. Variation of peptiside concentrations cause the changing of potential membrane value. Increasing of peptiside concentration cause decreasing of potential membrane. Its may due to some blocking of channel and other protein by peptiside molecule so the permeability of membrane to ions is decrease. The results of our experiment show that the increasing of peptiside concentration cause decreasing of the membrane potential value. For peptiside concentration of  0.4% decrease potential membrane to -28 ± 5 mV. It means that the increasing peptiside concentration cause significanly decrease in potential membrane which may be used for prediction of water polution level.
Effects of Visible Spectrum on Potential Differential Response to Soybean Sprouts (Glycine max (L.) Merill) during photosynthesis Farida Wahyuni; Unggul P. Juswono; Kusharto Kusharto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (899.497 KB) | DOI: 10.21776/ub.natural-b.2012.001.03.3

Abstract

The absorption visible light spectrum by chlorophyll cause excited electron. The transition of electron in photosynthesis process result electrical potential difference response which can be utilized to spur metabolism process in the plants itself. This research used the seedling of Glycine max (L.) and the varietas is sindoro. The measurement of the potential difference response is done by adding of visible light (red, yellow, green, blue and violet) alternately to the bean sprouts of Glycine max (L.) Merill. The light distribution is being done for 1 second, 5 second and constantly. The result of the research showed the effect of the visible light distribution constantly in the photosynthesis process. In the red light occurs maximum potential difference response. In peak I resulted -147,3 mV and peak II resulted -137 mV. The minimum potential difference response is resulted by green light. in peak I resulted -68,8 mV and peak II resulted -56,75 mV. The effect of the visible light distribution during any time in the photosynthesis process. In the red light occurs maximum potential difference response. In peak I resulted -119,2 mV for 1 second and -146 Mv for 5 second. In peak II resulted - 112,3 mV for 1 second and -125,5 mV for 5 second. The minimum potential difference response is resulted by green light. In peak I resulted -64,75 mV for 1 second, -69,5 mV for 5 second, and in peak II resulted -55,9 mV for 1 second and -56 mV for 5 second.