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All Journal Jurnal Ternak Tropika
Suyadi Suyadi
Faculty of Animal Science, Universitas Brawijaya, Kota Malang, Jawa Timur, 65145, Indonesia

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Single Nucleotide Polymorphims (snps) Identification of Inhibin Sub Unit-α (inha) Gene on Madura Bulls Irida Novianti; Chairdin Dwi Nugraha; Rafika Febriani Putri; Ahmad Furqon; Wike Andre Septian; S. Rahayu; V. M. A. Nurgiartiningsih; Suyadi Suyadi
TERNAK TROPIKA Journal of Tropical Animal Production Vol 22, No 1 (2021): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2021.022.01.10

Abstract

INHA gene is a gene that is suggested to have role in reproductive system. Single Nucleotide Polymorphisms (SNPs) of Madura Bulls were identified in this study. Polymerase Chain Reactions (PCR) was used to amplify INHA gene region and MEGA 7 program was utilized to align the amplified region sequences with sequence from Ensembl database. Four SNPs found in INHA and they are located at the first exon. Two SNPs were misssense mutations that causing the substitution of amino acid leucine21 by proline, and amino acid valine63 by methionine and the other two SNPs were synonymous mutation. One of the synonymous SNPs was a novel mutation. Based on those identified SNPs, they could be suggested as potential candidate markers of reproduction traits for Madura bulls. Moreover, through heterozygosity value from the observed bulls, it was indicated that the genotype was varied in population. Therefore a molecular selection program could be designed to determine the Madura superior bull.
Co-Authors Ahmad Furqon Chairdin Dwi Nugraha Irida Novianti Nurgiartiningsih, V. M. A. Rafika Febriani Putri S. Rahayu Wike andre Septian