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Ema Mulyawati
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
Dentistry

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Effect of 35% sodium ascorbate on calcium and phosphorus loss in dentin bleached by 35% hydrogen peroxide Tunjung Nugraheni; Nuryono Nuryono; Siti Sunarintyas; Ema Mulyawati
Majalah Kedokteran Gigi Indonesia Vol 7, No 1 (2021): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.46946

Abstract

Post bleaching procedures often have free radicals trapped in dentin tubule and interprismatic enamel, leading to demineralization and denaturation. Sodium ascorbate is an antioxidant substance known to bind residual free radicals to stop demineralization and denaturation. The purpose of the study was to assess the calcium and phosphorus loss from the dentin surface following bleaching by 35% hydrogen peroxide and reversal by 35% sodium ascorbate andthe surface structure of the dentin. Six sound premolars were divided into their crown and root sections, with the crown subsequently being cut into four equal parts to obtain 24 samples. The calcium and phosphorus contents as well as the surface structure of the dentin were assessed using SEM-EDX. These were then divided into four groups, each containing six samples. Group A (control): the samples were bleached by 35% hydrogen peroxide, immersed in an artificial saliva, stored in an incubator at 37 °C for seven days. Group B: the samples were bleached by 35% hydrogen peroxide followed by the application of 35% sodium ascorbate for 5 minutes (once). Group C: the samples were bleached by 35% hydrogen peroxide followed by the applications of 35% sodium ascorbate (twice). Group D: the samples were bleached by 35% hydrogen peroxide followed by the applications of 35% sodium ascorbate (threetimes). The calcium and phosphorus contents as well as the surface structure of the dentin were re-assessed using SEM-EDX at the same locations. The results of one-way ANOVA indicated a difference in the calcium loss in the four treatment groups (p<0.05), but there was no difference in the phosphorus loss. An LSD test showed that there was a difference in the calcium loss between group A and groups C and D or between group B and groups C and D. The dentin tubules in group A were larger than group B, C and D. The frequency of 35% sodium ascorbate application had an effect on the calcium loss and the surface structure in the dentin bleached by 35% hydrogen peroxide.
The effect of sisal (Agave Sisalana) nanofiber in epoxy resin sealer on root canal obturation’s push-out bond strength Astriani Amanda; Diatri Nari Ratih; Ema Mulyawati
Majalah Kedokteran Gigi Indonesia Vol 7, No 3 (2021): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.62691

Abstract

Sisal nanofiber can be used as an additional filler to increase the adhesion strength of the resin epoxy sealer. The aim of this study was to observe the effect of sisal nanofiber addition to epoxy resin sealer on push-out bond strength of root canal obturation material against the root canals. Twenty-five mandibular premolars (n= 25) were prepared until file F3 and obturated using 0%, 0.25%, 0.5%, 0.75%, and 1% sisal nanofiber sealer. After the specimens were stored at incubator, they were horizontally sectioned at apical third. Root sections were tested with push-out technique, and observed under a stereo microscope to determine the failure type. Data were analyzed with one-way ANOVA and LSD pos hoc at 95% confidence level (α=0.05). A one-way ANOVA test revealed that the addition of sisal nanofiber in epoxy resin sealer had a significant effect on the push out bond strength of the obturation material against the root canals. The failure type was observed predominantly in the cohesive type and the lowest in the adhesive type. The addition of sisal nanofiber to the epoxy resin sealer could increase the push-out bond strength of the obturation material against the root canals. Epoxy resin sealers with the addition of sisal nanofiber at a concentration group of 0.75% resulted in the highest push-out strength of root canal obturation materials followed by 0.5% concentration group.
Effects of sisal nanofiber addition to epoxy resin-based sealer on its antibacterial power against Enterococcus faecalis Heny Retno Mayangsari; Tunjung Nugraheni; Ema Mulyawati
Majalah Kedokteran Gigi Indonesia Vol 7, No 3 (2021): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.62867

Abstract

Sealer is one of root canal filler materials which has been developed and has an antibacterial agent to keep root canal sterile during and after an obturation process. This study aimed to find out the effect of sisal nanofiber addition to resin epoxy-based sealer on the antibacterial power against Enterococccus faecalis (E. faecalis) which is known as adaptive and potent bacteria which can be both aerobic and anaerobic. Sisal fibers were processed through many stages to make it nano sized (scouring, bleaching, neutralization, ultrasonification, and freeze-drying). Once nano-sized sisal fibers had been obtained, they were then mixed with sealer powder (AH26) in different concentrations: powder 0%, 0.25%, 0.5%, 0.75% and 1%. These concentrations were chosen based on preliminary research for reasonable contact angle measurement of sisal-sealer mixture. Antibacterial effect was examined using the diffusion method, each concentration was tested in 5 petri dishes which were planted with 1.5 X 108 CFU/ml E.faecalis bacteria. Each dish consisted of 5 holes (6 mm in diameter), each hole represented each concentration of nano sisal and sealer which were mixed until homogenous for 3 minutes before added to each hole. The dishes were then incubated for 48 hours at 37 °C. Inhibitory zones were measured, and analyzed using one-way ANOVA. The one-way ANOVA result showed that p=0.502 (p>0.05), meaning that the sisal nanofiber addition to epoxy resin-based sealer had no effect on E.faecalis inhibition. Thus, there was no effect of sisal nanofiber addition to epoxy resin-based sealer on E. faecalis.
Co-Authors Astriani Amanda Diatri Nari Ratih Heny Retno Mayangsari Nuryono Nuryono Siti Sunarintyas Tunjung Nugraheni