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All Journal Indonesian Journal of Biotechnology The Indonesian Biomedical Journal IGKOJEI: Jurnal Pengabdian Masyarakat
Hevi Wihadmadyatami
Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Public Health Social Sciences Veterinary

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In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system Aris Haryanto; Hevi Wihadmadyatami; Nastiti Wijayanti
Indonesian Journal of Biotechnology Vol 25, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.54703

Abstract

The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV).  The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmids carrying the gene coding for the recombinant F protein of NDV were obtained. Selected recombinant plasmids were then in vitro by using a cell-free protein expression system followed by visualization of the recombinant F protein on a 12% SDS-PAGE gel both by Coomassie Brilliant Blue staining and Western blotting. Recombinant F protein was successfully in vitro expressed by using a cell-free protein expression system as indicated by a specific single protein band with a molecular mass of 25.6 kDa.
PENDAMPINGAN PEMBELAJARAN BIOLOGI ANATOMI HEWAN SECARA DARING UNTUK SEKOLAH-SEKOLAH DASAR GUGUS VI DI DESA TUKSONO, SENTOLO, KULON PROGO, YOGYAKARTA : Online Animal Anatomy Biology Learning Assistance for Cluster VI Elementary Schools in Tuksono Village, Sentolo, Kulon Progo, Yogyakarta Arvendi Rachma Jadi; Hery Wijayanto; Dwi Liliek Kusindarta; Vista Budiariati; Tri Wahyu Pangestiningsih; Teguh Budipitojo; Ariana; Hevi Wihadmadyatami
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2022): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v3i2.301

Abstract

ABSTRACT  The problem faced during the new normal period of the Covid-19 pandemic for students is to restore interest in learning that has been done at home. Additional efforts are needed to reduce these concerns, such as mentoring for learning outside of school. Community service in the form of learning assistance is carried out with the aim of providing new experiences, information, and knowledge outside of learning at school. The schools participating in the service activities are schools from Cluster VI Tuksono Village, Sentolo, Kulon Progo, Yogyakarta. This activity involves student assistants from the Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada with assistance for a certain period. Implementation of online activities providing materials and discussions that have been adapted to the competencies needed in schools. The material given is an introduction to vertebrates macroscopically and microscopically. Students will be divided into several classes and each has an assistant and a co-lecturer. The activity takes place from September to November 2021. The results of the questionnaire show that this learning assistance activity is very interesting (60%) and very useful (69%) for students and teachers in their schools.  Keywords: Animal, Biology Anatomy; Elementary school; Learning; Online   ABSTRAK  Permasalahan yang dihadapi pada masa normal baru pandemi Covid-19 bagi peserta didik adalah mengembalikan minat belajar yang selama ini dilakukan di rumah. Usaha untuk mengurangi keprihatinan tersebut diperlukan seperti pendampingan belajar luar sekolah. Pengabdian kepada masyarakat berupa pendampingan pembelajaran dilaksanakan dengan tujuan memberikan pengalaman, informasi, dan pengetahuan baru di luar pembelajaran di sekolah. Sekolah yang berpartisipasi dalam kegiatan pengabdian adalah sekolah-sekolah dari Gugus VI Desa Tuksono, Sentolo, Kulon Progo, Yogyakarta. Kegiatan ini melibatkan asisten mahasiswa Departemen Anatomi Fakultas Kedokteran Hewan Universitas Gadjah Mada dengan pendampingan selama periode tertentu. Pelaksanaan kegiatan daring pemberian materi dan diskusi yang sudah disesuaikan dengan kompetensi yang dibutuhkan di sekolah. Materi yang diberikan berupa pengenalan vertebrata secara makroskopik dan mikroskopik. Peserta didik akan dibagi menjadi beberapa kelas dan masing-masing memiliki satu asisten dan dosen pendamping. Kegiatan berlangsung dari bulan September sampai November 2021. Hasil kuisioner menunjukkan kegiatan pendampingan pembelajaran ini sangat menarik (60%) dan sangat bermanfaat (69%) bagi para peserta didik dan guru di sekolahnya. Kata kunci: Anatomi Biologi; Daring; Hewan; Pembelajaran; Sekolah Dasar
2-(3-(chloromethyl)benzoyloxy)benzoic Acid Increases CD4+ Regulatory T-Cell Population and FoxP3 Expression in Lipopolysaccharide-induced Mice Yudy Tjahjono; Caroline Caroline; Jusak Nugraha; Kuncoro Foe; Srikanth Karnati; Süleyman Ergün; Nico Jafet; Oryza Chrisantia; I Made Andika Bara Kusuma; Hendy Wijaya; Wuryanto Hadinugroho; Dwi Aris Agung Nugrahaningsih; Dwi Liliek Kusindarta; Yufita Ratnasari Wilianto; Senny Yesery Esar; Hevi Wihadmadyatami
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2429

Abstract

BACKGROUND: Lipopolysaccharide (LPS) has been reported to increase CD4+ regulatory T-cell (CD4+ Treg) populations. Acetylsalicylic acid (ASA) has been reported to have immunomodulatory activity, but it may induce chronic gastric ulceration. Another salicylic acid-bearing compound, 2-(3-(chloromethyl)benzoyloxy)benzoic acid (3-CH2Cl), has been reported to have less gastric mucosal damage. However, the effect of 3-CH2Cl on CD4+ Tregs in LPS-induced mice is still unknown. Therefore, the present study was conducted to investigate the immunomodulatory effect of 3-CH2Cl on CD4+ T-cell and CD4+ Treg populations as well as FoxP3 expression in LPS-induced mice.METHODS: Synthesis of 3-CH2Cl was performed by mixing salicylic acid and chloromethylbenzoylchloride with the catalyzation of pyridine, acetone and heat. The 3-CH2Cl tablets were prepared using direct compression method. After intraperitoneal injection of 1 mg/kg BW LPS to mice, 60 mg/kg BW ASA or 60 mg/kg BW 3-CH2Cl was given orally for 3 days. The splenocyte was obtained through splenectomy and collagenase digestion. The population of CD4+ T-cells and CD4+ Tregs, as well as the splenic FoxP3 expression were determined using flow cytometry technique.RESULTS: CD4+ T-cell populations in mice treated with LPS and 3-CH2Cl or ASA were lower than those treated with LPS merely. Meanwhile, CD4+ Treg populations and FoxP3 expression levels in mice treated with LPS and 3-CH2Cl or ASA were higher than those treated with LPS merely.CONCLUSION: Since 3-CH2Cl could decrease CD4+ T-cell population and increase CD4+ Treg population mediated by the increase of FoxP3 expression in LPS-induced inflammation, it may act as a potential therapeutic drug to reduce inflammatory conditions.KEYWORDS: 2-(3-(chloromethyl)benzoyloxy)benzoic acid, acetylsalicylic acid, CD4, T-regulatory cells, FoxP3, LPS
Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization Dinda Aliffia; Dinda Ayu Ramadhani; Widya Wasityastuti; Dewi Ratih Tirto Sari; Ulayatul Kustiati; Hevi Wihadmadyatami; Dwi Liliek Kusindarta
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2415

Abstract

BACKGROUND: Neurodegenerative diseases are characterized by the loss of neuronal function in the nervous system. In recent years, more than 45 million people worldwide have suffered from progressive loss of memory and cognitive functions caused by Alzheimer’s disease. Ocimum sanctum is one of the medicinal plants known to have neuroprotective abilities. This study was conducted to elucidate the anti-apoptotic effects of ethanolic extract of O. sanctum (EEOS) on PC12 and SH-SY5Y cells as well as interaction between main compounds of EEOS and β-amyloid (Aβ) peptide through in silico molecular docking.METHODS: The viability of TMT-induced PC12 and SH-SY5Y cells was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and acridine orange/propidium iodide staining. Cell proliferation rate was measured with cell counting kit-8 (CCK-8) assay. Nuclear fragmentation was observed with Hoechst 33342 staining. Caspase -3 and -9 expressions were measured using enzyme-linked immunosorbent assay. Interactions between main compounds of EEOS and Aβ were visualized with in silico molecular docking.RESULTS: EEOS had the potential effect of maintaining cell viability, preventing the cell’s morphological changes, and inhibiting apoptosis via the caspase pathway in PC12 and SH-SY5Y cells. Meanwhile, flavonoid K, phenol, eugenol could interact with the active site of Aβ through hydrogen-bonding and hydrophobic interactions.CONCLUSION: EEOS could prevent neuronal cell apoptosis via downregulation of caspase-3 and -9. Main compounds of EEOS could interact with the active site of Aβ, and thereby might inhibit Aβ oligomerization. Thus, EEOS and its main compounds could be potential as neuroprotective agents for preventing neurodegenerative diseases.KEYWORDS: Ocimum sanctum, anti-apoptotic, β-amyloid, caspase, neurodegeneration
Co-Authors Ariana Aris Haryanto Arvendi Rachma Jadi Budiariati, Vista Caroline Caroline Dewi Ratih Tirto Sari Dinda Aliffia Dinda Ayu Ramadhani Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Foe, Kuncoro Hendy Wijaya Hery Wijayanto I Made Andika Bara Kusuma Jusak Nugraha Nastiti Wijayanti Nico Jafet Oryza Chrisantia Senny Yesery Esar Srikanth Karnati Süleyman Ergün Teguh Budipitojo Tri Wahyu Pangestiningsih Ulayatul Kustiati Widya Wasityastuti Wuryanto Hadinugroho Yudy Tjahjono Yufita Ratnasari Wilianto