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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Riset Akuakultur

Daniar Kusumawati

Balai Besar Penelitian dan Pengembangan Budidaya Laut, Gondol

Author-ID : 2890846

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Library & Information Science

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Articles

GROW OUT CULTURE OF CORAL TROUT Plectropomu leopardus IN FLOATING NET CAGES WITH DIFFERENT FEEDING FREQUENCIES Ketut Maha Setiawati; . Zafran; Daniar Kusumawati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

A study on the effect of feeding frequency on coral trout culture Plectropomus leopardus was conducted in floating net cages. The aim of this study was to determine an optimum feeding frequency for better growth rate and feed conversion ratio. The experiment was divided into two stages, i.e.; using fish with a range of body weight of 24 to 100 g (1st stage), and fish with a range of 100 to150 g (2nd stage). The first stage, consisted of four treatments of feeding frequencies, i.e. four times a day (4/1), three times a day (3/1), twice a day (2/1), and once a day (1/1). For the second stage experiment, the treatments applied were, three times a day (3/1), twice a day (2/1), once a day (1/1), and once in two days (1/2). Each treatment was in triplicate. At each feeding time, experimental fish were fed with dry pellets to apparent satiation. Both first and second experimental stages showed that the treatments of twice a day (2/1) feeding frequency attained significantly higher growth rates and lower feed conversion ratios than other treatments (P<0.05). Survival rate at the first stage (4/1), three times a day (3/1), twice a day (2/1), higher than (1/1) (P<0.05). But in the second stage the survival rate is not significant. The most optimum feeding frequencies for this species is 2/1 for the fish of 24 to 150 g BW.

THE EFFECTS OF FASTING DURATION AND DIFFERENT DENSITIES OF GROUPER SEED TRANSPORTED IN CLOSED SYSTEM Suko Ismi; Daniar Kusumawati; Yasmina Nirmala Asih
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Indonesia is an archipelagic country with many locations suit for aquaculture. Grouper hatcheries are still concentrated in a few locations. Therefore, appropriate technology to transport live grouper seed is needed to ensure the survival and health of the seed. This study aimed to determine the duration of fasting before transportation and the optimum density of grouper seed during transportation. Transportation was conducted in closed system, transported using a car, for 24 hours. Grouper seed used for the experiment was cantik hybrid grouper, a cross-breed between female tiger grouper (Epinephelus fuscoguttatus) and male marbled grouper (Epinephelus microdon) with an average of size 7.0±0.32 cm. There were 2 experiments in this study. Experiment 1 was different durations of fasting before transprotation: A. 12 hours, B. 24 hours, C. 36 hours and D. 48 hours with the density of 150 seeds/boxes. The result of the first experiment was used to determine the experimental design of experiment 2, which consisted of 4 different densities per boxes: A. 125 seeds, B. 150 seeds, C. 175 seeds and D. 200 seeds. Both of the experiments were conducted in triplicates. The seeds were packed in plastic bags with a thickness 0.08 mm, length 120 cm x width 53 cm, filled with 10 liters of sea water. The results showed that the duration of fasting from 36 to 48 hours had the highest survival rate. Polinomial relation y = -0.0439x2 + 3.7696x + 17.075 with the value of R² = 0.966 illustrated that the optimum duration of fasting was 44 hours. There was a significant difference in densities of seeds during transportation (P<0.05), where the higher density lead to the lower survival rate. The density of 125 seeds/boxes resulted in the highest survival rate, 99.0 ± 1.0%.

GROWTH AND BLOOD PROFILE OF CORAL TROUT GROUPER Plectropomus leopardus SEEDS IN DIFFERENT SALINITIES Ketut Maha Setiawati; Ketut Mahardika; A. A. Ketut Alit; Daniar Kusumawati; Indah Mastuti
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

The information about the optimum and tolerable salinity to support in the success of coral trout grouper’s seed culture is still limited until now. The aim of this study was to understand the effect of different salinity on growth, survival, feed conversion (FCR) and the profile of blood (hematocrit and hemoglobin) of coral trout grouper seed. The seed with total length 6.0 ± 0.5 cm and body weight 3.2±0.3 g were used as tested animals. Five different salinity treatments, namely: 10±1 ppt (A), 16±1 ppt (B), 22±1 ppt (C), 28±1 ppt (D), and the sea water (34 ppt) as a control (E) were undertaken in this study, with three replications for each treatment. Salinity values were reach by adding freshwater into the tested seawater. Water changing system in this study was performed in recirculation process, with 25% of water replacement every 7 days. The result of this study showed that the survival rate values were 100% for all treatments, indicating that coral trout grouper seed can be cultured in the range of salinity between 10 to 34 ppt. The highest total length and body weight were recorded from treatment C (22 ppt), as well as noted to gain the lowest FCR. Correspondingly, the seed in treatment C also founded to have in the greatest amount of hematocrit and hemoglobin. Optimum salinity for rearing of coral trout grouper seeds with total length 6 cm was 22 ppt. Keywords: salinity, growth, survival rate, feed conversion, blood profile

LAJU PENGOSONGAN ISI PERUT PADA IKAN KERAPU CANSIR (Epinephelus fuscoguttatus x Epinephelus corallicola) SEBAGAI INFORMASI AWAL DALAM PENENTUAN MANAJEMEN PEMBERIAN PAKAN Daniar Kusumawati; Suko Ismi
Jurnal Riset Akuakultur Vol 9, No 3 (2014): (Desember 2014)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Informasi mengenai laju pengosongan isi perut suatu jenis ikan sangat diperlukan untuk meningkatkan keefektifan dan keefisienan pemanfaatan pakan. Dalam penelitian ini dilakukan uji untuk mengetahui lama pengosongan isi perut benih kerapu cansir yaitu hibrida dari kerapu macan (Epinephelus fuscoguttatus) x kerapu pasir (Epinephelus corallicola) terhadap jenis pakan pelet. Benih kerapu cansir sejumlah 50 ekor dengan kisaran panjang 8,00 ± 0,76 cm terlebih dahulu dipuasakan selama 48 jam dan kemudian ikan diberi makan secara adlibitum. Ikan selanjutnya dibedah sejumlah lima ekor pada jam ke-0, 6, 12, 15, 18, 21, 24, 30, 35, dan 42. Berdasarkan hasil pengamatan diketahui bahwa lama pengosongan isi perut berdasarkan persentase derajat kepenuhan isi perut per satuan waktu (ISC) pada ikan kerapu cansir ukuran 8,00 ± 0,76 cm terhadap jenis pakan pelet mencapai 28 jam dengan total laju kecepatan pengosongan lambung rata-rata terhadap penurunan persentase pakan sebesar 2,9%/jam. Berdasarkan lama pengosongan isi perutnya, ikan kerapu cansir cukup diberikan pakan pelet dengan frekuensi satu kali per hari.

PERAN GEN AIM1 DAN INTENSITAS CAHAYA TERHADAP KARAKTER POLA PIGMEN IKAN BADUT HITAM (Amphiprion percula) Daniar Kusumawati; S. Permana; Ketut Maha Setiawati; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 7, No 2 (2012): (Agustus 2012)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Pola pigmen merupakan faktor utama yang menentukan tingginya kualitas ikan hias. Pada benih-benih F1 populasi budidaya ikan badut hitam (Amphiprion percula) diketahui mengalami degeneratif pola pigmen yang menyimpang dibandingkan dengan populasi di alam. Pola pigmen merupakan salah satu karakter fenotip yang diturunkan di mana ekspresinya bergantung pada interaksi genetik dengan faktor eksternal yaitu lingkungan. Tujuan dari penelitian ini adalah untuk mengkaji peran gen yaitu Aim1 yang mengontrol sintesis melanin dan kondisi lingkungan dalam menginduksi pola pigmen hitam ikan badut hitam (Amphiprion percula). Analisis gen Aim1 dilakukan dengan menggunakan program speedy PCR dilanjutkan dengan SSCP (single strand confirmation polymorphism) untuk mengidentifikasi variasi genetik dari gen Aim1. Analisis SDS Page dilakukan untuk mengetahui peranan lingkungan terhadap profil protein yang disintesis. Berdasarkan hasil penelitian diketahui bahwa primer Aim1 memiliki sisi pengenalan pada whole genom ikan badut hitam (Amphiprion percula) pada target sequence 45 bp. Berdasarkan analisis SSCP profil fragmentasi amplicon primer Aim1 pada masing-masing tingkatan pada populasi budidaya homolog dengan populasi alam, sehingga gen Aim1 bukan merupakan gen yang mengontrol fenomena degeneratif pola pigmen ikan badut hitam (Amphiprion percula). Peran lingkungan yaitu cahaya memberikan pengaruh positif dalam menginduksi pola pigmen melalui stimulus pada sistem neuron dan migrasi melanophore.

PERKEMBANGAN EMBRIO DAN LARVA IKAN LETTER SIX, Paracanthurus hepatus Daniar Kusumawati; Ketut Maha Setiawati; Bambang Priono
Jurnal Riset Akuakultur Vol 10, No 2 (2015): (Juni 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Dalam upaya memperkaya populasi ikan letter six (Paracanthurus hepatus) di alam yang telah mendekati kepunahan akibat adanya illegal fishing maka dilakukan usaha perbenihan. Kegiatan perbenihan ikan letter six memerlukan informasi awal tentang perkembangan telur dan pemeliharaan larva yang nantinya akan digunakan sebagai acuan dasar dalam menentukan pengembangan teknik pemeliharaan larva ikan letter six yang tepat. Koleksi induk ikan letter six hasil tangkapan di alam dari perairan Maluku dan Papua. Induk letter six dipelihara dalam tangki policarbonat bulat berkapasitas dua ton dengan sistem air mengalir. Selama pemeliharaan, induk letter six mampu memijah secara alami. Durasi perkembangan embrio hingga menetas memerlukan waktu 22 jam 24 menit pada suhu normal air laut 29°C. Penyerapan kuning telur optimum terjadi pada hari kedua dan maksimum terserap habis pada hari ketiga, sedangkan butir minyak terserap habis tepat pada hari ketiga. Korelasi pertumbuhan panjang dan tinggi larva mengikuti hubungan alometrik negatif di mana konstanta pertumbuhan b = 0,6629 (b < 1). Berdasarkan perkembangan larva, umur D-2 (terbentuk bintik mata) mulai diberikan pakan alami berupa plankton dan pada D-3 (bukaan mulut) mulai diberikan zooplankton yaitu rotifer. Dengan metode pemeliharaan ini, larva ikan letter six mati pada D-25 sehingga perlu adanya perbaikan metode pemeliharaan melalui pendekatan lingkungan maupun pakan.

Co-Authors . Zafran A. A. Ketut Alit Bambang Priono Haryanti Haryanti Indah Mastuti Ketut Maha Setiawati Ketut Mahardika S. Permana Suko Ismi Yasmina Nirmala Asih

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