Kadek Ayu Intan Sari
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KAPASITAS ANTIOKSIDAN SENYAWA GOLONGAN TRITERPENOID PADA DAUN PRANAJIWA (Euchresta horsfieldii lesch benn) Kadek Ayu Intan Sari; I Wayan Gede Gunawan; Ketut Gede Dharma Putra
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.166 KB) | DOI: 10.24843/JCHEM.2015.v09.i01.p10

Abstract

Isolation, identification, and anti-oxidant assay of triterpenoid compound  from Euchresta horsfieldii lesch benn) have been conducted. Maseration of 600 g pranajiwa leaves powder with n-hexane produced 6,1 g n-hexane extract which gave yellow to purple colour change with Lieberman-Burchard reagent. The separation with column chromatography using silica gel 60 obtained 3 fractions (F1, F2, F3) which were then analyzed with triterpenoid test. The positive fraction of triterpenoid (F1) produced 2 g yellow isolate. Infrared spectra indicated that the isolate was a triterpenoid, with characteristic functional groups of OH, –CH, C=O, –C=C, –CH2, –CH3, and C–O alcohol. Ultraviolet-visible spectra showed broad peak at 217 nm. The result of anti-oxidant capacity test of triterpenoid was 94,67%, showing that triterpenoid from pranajiwa leaves can be use as an anti-oxidant.