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All Journal Medical Journal of Indonesia INDONESIAN JOURNAL OF PHARMACY
Leonardus B.S. Kardono
International Center for Interdisciplinary and Advanced Research – LIPI, Jakarta, Indonesia
Medicine & Pharmacology

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Journal : Medical Journal of Indonesia

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Cytotoxic assay of endophytic fungus 1.2.11 secondary metabolites from Brucea javanica (L) Merr towards cancer cell in vitro Sudarmono, Pratiwi; Utji, Robert; Kardono, Leonardus B.S.; Kumala, Shirly
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.968 KB) | DOI: 10.13181/mji.v15i3.228

Abstract

Cytotoxic assay of secondary metabolite endophytic fungus 1.2.11 from Brucea javanica (L) Merr has been carried out. Brucea javanica fruit collected from Cianjur was used in this experiment. Cytotoxic assay was done on Raji, NS-1, HeLa and Vero cells. The observation was done for 24 hours and also for 48 hours. IC50 was calculated using the Rich and Muench theory. To observe the working mechanism of cytotoxic process, DNA staining with etidium bromide and acridine orange was conducted. The cytotoxic assay of endophytic fungi 1.2.11 showed an IC50 of 58.35 μg/ml, 88.39 μg/ml on Raji cell,; 162.09 μg/ml, 66.24 μg/ml on NS cell; 361.21 μg/ml, 219.97 μg/ml on HeLa cell; and lastly 1075.18 μg/ml, 656.82 μg/ml on Vero cell after 24 and 48 hour incubation respectively. The results of this study showed that secondary metabolite of endophytic fungus 1.2.11 has selective cytotoxic effect towards cancer cell and also showed that it might cause apoptosis in NS-1cell. (Med J Indones 2006; 15:137-44) Keywords: Brucea javanica (L.) Merr, endophytic microbe, Cytotoxic assay, endophytic isolate 1.2.11, apoptosis
Co-Authors Akhmad Darmawan Pratiwi Sudarmono Robert Utji Shirly Kumala Sofa Fajriah Soleh Kosela Wahyudi P. Suwarso, Wahyudi P.