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Phytochemical Screening and Toxicological Evaluation Using Brine Shrimp Lethality Test (BSLT) of Some Fractions of Prasman Leaves (Eupatorium triplinerve V) Extract Kumala, Shirly; Sapitri, Dwi Windi
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

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Prasman leaves (Eupatororium triplinerve V) was well documented to have anti cancer benefit in Indonesian traditional medicine history. However, there were noscientific studies including toxicological assessment on the planextract.The purpose of this study was to investigate thetoxicological effect of some fractions of Prasman leavesmethanol extract. Phytochemical screening by the Farnsworth method on powder and some fraction of the methanol extractwere conducted followed by toxicitytest using the “Brine Shrimp Lethality” test (BSLT) method. In the current study results, the phytochemical screening showed the presence of flavonoid, saponin, coumarin, tannin, steroidandvolatileoil.LC50ofthen-hexanefraction238.660g/mL,ethylacetatefraction 24.420g/mL,andn3butanol64.100g/mL.
Cytotoxic assay of endophytic fungus 1.2.11 secondary metabolites from Brucea javanica (L) Merr towards cancer cell in vitro Sudarmono, Pratiwi; Utji, Robert; Kardono, Leonardus B.S.; Kumala, Shirly
Medical Journal of Indonesia Vol 15, No 3 (2006): July-September
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Cytotoxic assay of secondary metabolite endophytic fungus 1.2.11 from Brucea javanica (L) Merr has been carried out. Brucea javanica fruit collected from Cianjur was used in this experiment. Cytotoxic assay was done on Raji, NS-1, HeLa and Vero cells. The observation was done for 24 hours and also for 48 hours. IC50 was calculated using the Rich and Muench theory. To observe the working mechanism of cytotoxic process, DNA staining with etidium bromide and acridine orange was conducted. The cytotoxic assay of endophytic fungi 1.2.11 showed an IC50 of 58.35 μg/ml, 88.39 μg/ml on Raji cell,; 162.09 μg/ml, 66.24 μg/ml on NS cell; 361.21 μg/ml, 219.97 μg/ml on HeLa cell; and lastly 1075.18 μg/ml, 656.82 μg/ml on Vero cell after 24 and 48 hour incubation respectively. The results of this study showed that secondary metabolite of endophytic fungus 1.2.11 has selective cytotoxic effect towards cancer cell and also showed that it might cause apoptosis in NS-1cell. (Med J Indones 2006; 15:137-44) Keywords: Brucea javanica (L.) Merr, endophytic microbe, Cytotoxic assay, endophytic isolate 1.2.11, apoptosis
EFEK ANTIBAKTERI EKSTRAK ETANOL DAUN CENGKEH (Eugenia aromatic L.) Kumala, Shirly; Indriani, Dian
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 2 (2008)
Publisher : Indonesian Research Gateway

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The effect of antibacterial from ethanol  extrcat Eugenia aromatica towards Gram positive and negative bacteria have been carried out. This study using diffusion method. The results shows the concentration of the extract 10 % and 20 % that has the effect to inhibit the growth of bacteria (Staphylococcus aureus, Bacillus subtillis and  Escherichia coli , Salmonella  parathyposa.) while the 1 % concetrration has no effect.  The diameter of inhibition zone of Ampicillin  as positive control has more bigger than the extract. The statistic results show there are significant difference between the concentration extract and diameter of inhibition zone for each bacteria. ABSTRAK Telah dilakukan penelitian efek antibakteri ekstrak etanol daun cengkeh (Eugenia aromatica) dengan menggunakan penyari etanol 96% terhadap bakteri Gram positif (Staphylococcus aureus dan Bacillus snbtilis) dan bakteri Gram negatif (Escherichia coli dan Salmonella paratyphi) dengan menggunakan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak daun cengkeh menunjukkan efek antibakteri terhadap bakteri Staphylococcus aureus, Bacillus subtilis, Escherichia coli, dan Salmonella paratyphi. Efek antibakteri dimulai pada konsentrasi 10%, sedangkan pada konsentrasi 1% tidak memberikan efek. Hasil penelitian menunjukkan diameter daerah hambat yang lebih kecil bila dibandingkan dengan diameter daerah hambat dari antibiotika ampisilin yang digunakan sebagai kontrol positif.. Dari analisis data dalam penelitian menggunakan perhitungan analisis statistik anova satu arah, hasil yang diperoleh menunjukkan adanya perbedaan bermakna antara konsentrasi ekatrak daun cengkeh dengan diameter daerah hambat terhadap masingmasing bakteri.
PENGARUH PEMBERIAN REBUSAN KAYU SECANG (Caesalpinia sappan L.) TERHADAP MENCIT YANG DIINFEKSI BAKTERI Escherichia coli Kumala, Shirly; Yuliani, .; Tulus, Didik
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 4 (2009)
Publisher : Indonesian Research Gateway

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The research has done using two methods.Method A treatment was conduct 2 hours after infecting the mice with the Escherichia coli,  while in the B method, the tratment was given to the mice after 24 hours. In every method, there were six groups (K1 was normal control using  healthy mouce, K2 was negative control, infecting with bacteria without treatment with antibiotic,. and K3 was positive control using chloramphenicol) K4-K6 were the test group in this group were treated with different concentrations of “kayu secang”. After 3 days  of treatment, the peritonium fluid of the mice was taken followed by quantitative analysis using plate count and qualitative with bacteria reidentification. Quantitative analysis via method A demonstrated that a decreased of K4 39.65 %, K5 45.64 % and K6 44.74 %. in bacterial colony number was observed in the samples collected from K4. K5 and K6 respectively. Method B showed decreased K4 37.26 %, K5 43.13 % and K6 42.93 % in the colony number counted. The qualitative analysis using differential medium demonstrated metallic light that showed the isolated bacterial strain was Escherichia coli. ABSTRAK Penelitian dilakukan menggunakan 2 metode. Metode A pengobatan dilakukan 2 jam setelah bakteri diinfeksi ke mencit, sedangkan pada metode B pengobatan dilakukan setelah 24 jam infeksi. Setiap metode terdiri dari 6 kelompok, kelompok 1 (normal), kelompok 2 (kontrol negatif) hanya diinfeksi bakteri tanpa diobati, kelompok 3 (positif) diinfeksi bakteri dan diobati dengan antibiotika kloramfenikol, kelompok 4, 5, 6 diinfeksi bakteri dan diobati dengan  rebusan kayu secang dengan 3 konsentrasi yang berbeda. Pengobatan diberikan satu kali sehari selama 3 hari, setelah itu dilakukan pengambilan cairan intraperitonium dan diuji secara kuantitatif menggunakan metode plate count serta uji secara kualitatif dengan melakukan reidentifikasi bakteri. Hasil penelitian menunjukan rebusan kayu secang dengan konsentrasi 10 %, 20 % dan 50 % pada metode A secara uji kuantitatif dapat menghambat pertumbuhan bakteri Escherichia coli, dengan prosentase penurunan koloni bakteri pada K4 37,26 %, K5 43,13 % dan K6 42,93 %. Pada metode B, K4 37.26 %, K5 43.13 % dan K6 42.93 % Pengamatan metode A dan B secara kualitatif  pada  media diferensial Eosin Methylen Blue agar, menunjukkan kilap logam metal.
POLA RESISTENSI ANTIBIOTIK TERHADAP ISOLAT BAKTERI SPUTUM PENDERITA TERSANGKA INFEKSI SALURAN NAFAS BAWAH Kumala, Shirly; Pasanema, Dimas A. M.; Mardiastuti, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 5, No 1 (2010)
Publisher : Indonesian Research Gateway

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Lower respiratory tract infection (LRTI) was one type of lung infection that is commonly suffered by the indonesion society including “in patients” in hospital ward.  The percentage of lung infection case was found to be 60-80% of all case of lung related disease while the rest (20-40%) were classified as non-infectious lung disease.  Fast and accurate diagnosis followed by correct choice of antibiotics based on antibiotic resistance test would certainly help to establish accurate diagnosis and treatment. Studies were carried out in clinical microbiology laboratory in the Faculty of Medicine of University of Indonesia.  A total of 124 specimens were used in the study, contained sputtam excreted by patients diagnosed with lower respiratory tract infectious disease. Isolation and identification procedure was performed according to the Laboratory standard operational procedure (SOP).  Antibiotic resistance test was performed using “CAKRAM” diffusion techniques according to CLSI which is abbreviated as Clinical and Laboratory Standards Institute. 76 bacterial isolates were successfully isolated and identified.  Most of the bacteria found were identified as Klebsiella Pneumoniae and they were Erithromycin sensitive and Ticarsilin resistant (75.9%).   ABSTRAK Infeksi Saluran Nafas Bawah (ISNB) merupakan penyakit infeksi paru yang paling sering ditemukan di masyarakat maupun yang dirawat di Rumah Sakit, dan masih merupakan masalah kesehatan utama di seluruh dunia. Penyakit infeksi paru berkisar 60-80% dari seluruh penyakit paru, sedangkan sisanya (20-40%) merupakan penyakit non infeksi. Penentuan diagnosis secara cepat dan tepat serta pemilihan antibiotik berdasarkan uji resistensi akan sangat membantu dalam petanalaksanaan dan terapi. Penelitian dilakukan di Laboratorium Mikrobiologi Klinik Fakultas Kedokteran Universitas Indonesia, Jakarta. Spesimen yang digunakan berupa sputum penderita tersangka infeksi saluran nafas bawah, jumlah sampel 124. Isolasi dan identifikasi dilakukan sesuai standard operasional laboratorium, uji resistensi menggunakan difusi cakram menurut CLSI. Dari hasil isolasi diperoleh 76 isolat bakteri, yang terbanyak  adalah Klebsiella pneumoniae yang sangat sensitif terhadap Eritromisin dan resisten terhadap Tikarsilin (75,9%)
UJI AKTIVITAS ANTI-BAKTERI EKSTRAK ETIL ASETAT KEMBANG PUKUL EMPAT (Mirabilis jalapa L.) DENGAN METODE BIOAUTOGRAFI Kumala, Shirly; Tambunan, Risma Marisi; Mochtar, Dede
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 2 (2006)
Publisher : Indonesian Research Gateway

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Assay for anti-bacterial activity  of ethyl acetate extract from kembang pukul empat ( Mirabilis jalapa L) using bioautographic method has been investigated. Bioautographic methods was carried by put the chromatogram of the extract on the media that had been inoculated with bacteria. The bacteria used in this experiment were Staphylococcus aureus and Escherichia coli. The results shown anti-bacteria activity  toward Staphylococcus aureus has value of Rf 21, 24, 35, 45, 53, 65 and  68. While for Escherichia  coli has value of  Rf 24, 33, 35, 45 and 65. ABSTRAK Aktivitas antibakteri dari ekstrak etil asetat kembang pukul empat ( Mirabilis jalapa L) telah diteliti menggunakan metoda bioautografi. Metoda ini dilakukan dengan jalan meletakkan kromatogram ekstrak di atas media yang telah diinokulasi dengan bakteri uji Bakteri yang digunakan dalam penelitian ini adalah Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan bahwa aktivitas anti bakteri ekstrak terhadap bakteri uji Staphylococcus aureus mempunyai nilai Rf 21, 24, 35, 45, 53, 65 dan  68. Sedangkan terhadap Escherichia  coli nilai Rf adalah 24, 33, 35, 45 dan 65.
AKTIVITAS ANTIMIKROBA METABOLIT BIOAKTIF MIKROBA ENDOFITIK TANAMAN TRENGGULI (Cassia fistula L.) Kumala, Shirly; Shanny, Fransisca; Wahyudi, Priyo
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 2 (2006)
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Endophytic microbes are microorganisms that live symptomatically within the living tissue of host plants. Microbe as fungi, yeast and bacteria can associate with the plant; they can help the metabolism of the host plant and produce the potential secondary metabolite. The aims of this experiment were to investigate endophytic microbes that live in the Cassia fistula L (Trengguli) plant and their ability to produce bioactive metabolite as anti microbial agents. Direct seed inoculating technique was used by putting the plant sample on the surface of Nutrient Agar. The agar diffusion method using paper disc was applied to assay the anti microbial activity of bioactive metabolite. The results showed 7 isolate of bacteria had the anti microbial activity toward Staphylococcus aureus, Bacillus subtillis, Escherichia coli, Salmonella typhi and Candida albicans. Endophytic bacteria can be found in Cassia fistula L plant, which can produce potential bioactive metabolite as anti microbial agent. ABSTRAK Mikroba endofit adalah mikroorganisme yang hidup secara simptomatis di dalam jaringan hidup tanaman inangnya. Mikroba tertentu, misalnya jamur, ragi dan bakteri dapat berasosiasi dengan tanaman tersebut; mikroba-mikroba ini dapat membantu metabolisme tanaman inangnya dan memproduksi metabolit sekunder yang potensial. Tujuan penelitian ini adalah menyelidiki mikroba endofit yang tumbuh di dalam jaringan Trengguli (Cassia fistula L), dan kemampuannya untuk memproduksi  metabolit bioaktif  yang memiliki aktivitas antimikrobial. Dalam penelitian ini digunakan teknik inokulasi langsung dengan jalan meletakkan sampel tanaman langsung di atas permukaan Nutrient Agar. Metoda difusi agar menggunakan cakram kertas digunakan untuk menetapkan aktivitas antimicrobial dari metabolit aktif. Hasil penelitian menunjukkan bahwa 7 isolat bakteri memiliki aktivitas antimikrobial terhadap Staphylococcus aureus, Bacillus subtillis, Escherichia coli, Salmonella typhi dan Candida albicans. Dengan demikian dapat disimpulkan bahwa di dalam jaringan tanaman Cassia fistula L dapat ditemukan bakteri endofit yang dapat memproduksi metabolit bioaktif sebagai senyawa antimicrobial.
Alga Merah (Gracilaria verrucosa) sebagai Bahan Bakto Agar Kumala, Shirly; Sumarny, Ros; Rachmani, Rum; Ruswita, Atut
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 3 (2013)
Publisher : Indonesian Research Gateway

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The used of bacto agar in microbiological studies increased tremendously, however, up till now, to fullfill its high demant the scientist were stil relying on imported bacto agar eventhough domestic production of bacto agar was as goog and reliable as those produced commercially overseas. The current study we focuseon the production of bacto agar from red algae Gracilaria verrucosa using fell press technique; followed by quality analysis of the product. Red algae samples were collected from two different locations (Bekasi and Subang). Quality of the product was tested for its content, acid insoluble as content, overall as content, pH, gel strength and its ability to be use as culture media to culture Escherichia coli and Staphylococcus aureus. Microbiological test was performed via pour plate. The results clearlydemonstrated that red algae sample from Bekasi produced bacto agar that meets the criteria of commercial bacto agar. It has 10.3% water content, 3.9% overall as content, 0.4% acid insoluble as content, pH of 7.3 and gel strength of 600.8-602.8 g/square cm.Keywords : Bacto agar, red Algae, Gracilaria verrucosa, Microbe media
Efek Antimikroba dari Kapang Endofit Ranting Tanaman Biduri Kumala, Shirly
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

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ABSTRACT:Didymella bryniae strain MA71 endophytic fungus was isolatedfrom twigs of Biduri plant (Calotropis gigantea L.Dryand). It has anti yeastactivity and anti bacterial activity against gram positive and negative bacteria.Secondary metabolites were isolated via fermentation process using PDYmedia for 12 hr. Anti microbial activity of these fermentation product was assessedvia agar diffusion method using disk paper. Test result demonstratedthat extracellular secondary metabolites which was extracted using EtOAcwere active towards Staphylococcus aureus ATCC 25923, Escherichia coliATCC 25922 and Bacillus subtilis ATCC 6633. On the other hand, these EtOACextracted metabolites were also active against Candida albicans ATCC 10231.Its inhibitory zone were 17.3 mm, 17.2 mm, 17.41 mm and 11.6 mm respectively.Chloramphenicol was used as positive control for bacteria and nystatinfor yeast. Overall, the se condary metabolites produced by fermentation of endophyticfungi Didymella bryniae strain MA71 have broad spectrum of antibacterial and yeast activity.Keywords: endophytic Fungus, shaked fermentation, secondary metabolites,antimicrobials, and Calotropis gigantea L. Dryand  
Aktivitas Antibakteri dan Antioksidan Daun Pecut Kuda (Stachytarpheta jamaicensis L.) Secara Invitro Kumala, Shirly; Bekti, Novi Dyah Purnama
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 2 (2016)
Publisher : Indonesian Research Gateway

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Pecut Kuda leave (Stachytarpheta jamaicensis L.) was one of the many medicinal plants found in Indonesia and it has been widely used by the people in Indonesian to cure infection of tractus urinarius, pharingitis, hepatitis. Although, it has been very popular traditional medicine, very little scientific data were collected until now. This study focused on preliminary studies of antibacterial and antioxidant activity of Pecut Kuda leaves. Extracts were obtained using n-hexane, ethyl acetate and methanol. The antibacterial activity test was performed using the agar diffusion method against Staphylococcus aureus and Escherichia coli. While antioxidant activity was performed by the colorimetric method using DPPH radical reduction. The results showed that n-hexane extracts did not have either antibacterial or antioxidant activity. The ethyl acetate extract had zone inhibition (DDH) between 7 to 11.67 mm against S. aureus bacteria, whereas against E. coli with DDH between 7.67 to 10.83 mm, and had IC50 value of 182.97%. The methanol extract had DDH between 8.33 to 13.67 mm against S.aureus, whereas against E. coli bacteria with DDH between 9 to 14.67 mm, and IC50 value of 14.28%. In conclusion, ethyl acetate and methanol extract of Pecut kuda leaves have antioxidant and antibacterial activity against S. aureus and E. coli bacteria.
Co-Authors . Desi . Yuliani Abdul Aziz Setiawan Ahmad Subhan Anton Pratama Anwar Wahyudi Ariyani Kiranasari Ariyani Kiranasari Armansyah, Septiyana Lia Asih Widiyastuti Atut Ruswita Ayuditiawati, Meita Bekti, Novi Dyah Purnama Bekti, Novi Dyah Purnama Delina Hasan Deni Rahmat Dian Indriani Dian Ratih L. Didik Tulus Dimas Adrianto Dwi Hapsari Juniarti Dwi Windi Sapitri Elvina Dhiaul Iftitah Erlita Agustina Esti Mumpuni Febbyasi Megawaty Gabriel Gabriel Gabriel, Gabriel Gandes Winarni Halim, Marta Hasan, Delina Herru Setiawan Hersunaryati, Yetti Heru Santosa Heru Santosa Hesty Utami Hesty Utami R Hesty Utami R Huda, Badrul I Ketut Suada Iha Haryani Hatta Kurota Aini Lea P. Retnoningsih Leonardus B.S. Kardono Lestari Rahayu Mardiastuti, . Mardiastuti, . Marlin M. Raunsai Mochtar, Dede Mochtar, Dede Nancy Dewi Yuliana Nancy Raisa Nancy Raisa, Nancy Nur Aji Nurmaini Nurmaini Partomuan Simanjuntak Partomuan Simanjuntak Pasanema, Dimas A. M. Pasanema, Dimas A. M. Pasaribu, Rita Kristina Pieter Hazmen Pratiwi Sudarmono Prih Sarnianto Priyo Wahyudi R, Hesty Utami Rachmani, Rum Rachmani, Rum Restu Restiani Reubun, Yonathan Tri Atmodjo Reza Agung Sriwijaya Risma Marisi Tambunan, Risma Marisi Rita Kristina Pasaribu Robert Utji Rochimah Ros Sumarny Sarnianto, Prih Saru Noliqo Rangkuti Sesilia A. Keban Sesilia Andriani Keban Setiadi, Feri Setiyarini, Setiyarini Shanny, Fransisca Shanny, Fransisca Simanjuntak, Partomuan Siswa Setyahadi Siswa Setyahadi Sofi Nurmay Stiani Sofyan Ramani Suhaimi Suhaimi Suhaimi, . Syarifah Aini Syilvi Adini Teti Indrawati Teti Indrawati Teti Indrawati Umilawati Rawi Utami R, Hesty Violeta, Violeta Wahyudi Uun Yelfi Anwar Yusransyah Yusransyah