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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Irfan D. Prijambada
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Isolasi dan Seleksi Bakteri dari Sedimen Mangrove untuk Pembentukan Konsorsium Bakteri Perombak Dibenzofuran Yanisworo W. Ratih; Bostang Radjagukguk; Erni Martani; Irfan D. Prijambada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.115

Abstract

Dibenzofuran merupakan salah satu senyawa hidrokarbon aromatis polisiklik (HAP) yang mengandung oksigen. Paparannya di alam harus segera ditanggulangi karena dibenzofuran berperan sebagai prekursor bagi senyawa berkhlor turunannya yang bersifat lebih toksik. Dibenzofuran dapat dijadikan senyawa model karena beberapa bakteri perombak dibenzofuran juga mampu merombak senyawa mirip lainnya seperti dibenzodioksin, fluorena, fluorantena, dibenzofuran terkhlorinasi, fenantrena dan antrasena. Penelitian ini dilakukan untuk membentuk konsorsium bentukan yang mempunyai kemampuan tinggi dalam merombak dibenzofuran. Isolat bakteri diperoleh dari sedimen mangrove asal Balongan, Indramayu, Jawa Barat menggunakan medium mineral cair yang diperkaya dengan dibenzofuran sebagai satu-satunya sumber karbon dan energi. Total 12 isolat bakteri, GMYk-1, GMYk-2, GMYk-3, GMYk-4, GMYk-5, GMYs-1, GMYs-2, GMYs-3, GMYs-4, GMYs-5, GMYs-6 dan GMYs-7 berhasil diisolasi dari sedimen. Berdasarkan pengamatan terhadap keragaman isolat-isolat yang diperoleh serta interaksi di antara isolat dalam merombak dibenzofuran, empat isolat berhasil terseleksi untuk menyusun konsorsium bentukan. Isolat tersebut adalah GMYs-1, GMYs-6, GMYs-7 dan GMYk-1. Berdasarkan kemampuan merombak dibenzofuran dari kombinasi isolat-isolat yang disusun, biakan campuran GMYs-1- GMYs-6-GMYk-1 dipilih sebagai konsorsium bentukan. Konsorsium bentukan mempunyai kemampuan paling tinggi dalam merombak dibenzofuran.
Pengaruh Bahan Organik dan Suhu Pengeringan terhadap Ketahanan Hidup Aspergillus niger dalam Pupuk Pelet Bio-fosfat Yudi Sastro; Donny Widianto; Irfan D. Prijambada
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i2.2667

Abstract

This research was aimed to investigate the effect of organic matter and drying temperature on Aspergillus niger survivability in the rock phosphate pellet fertilizer namely bio-phosphate. The research was arranged by a completely randomized design 3x3x6. Addition of the mixing of tapioca waste, rice bran, and starch (BOC) and the humic substance (BH) in the bio-phosphate, and its drying temperature (SP) were the treatments. Aspergillus niger inoculums survivability in the bio-phosphate was determined using plating methods. The result showed that the addition of BOC decreased amount of A. niger in the bio-phosphate up to 28.0%, while the BH increased the amount of A. niger up to 24.4%. The ideal drying temperature of bio-phosphate pellet fertilizer was 600C.
Pengaruh Campuran Onggok, Sekam, Pati dan Bahan Humat terhadap Pertumbuhan Aspergillus niger dan Kemampuannya dalam Melarutkan Fosfat Yudi Sastro; Donny Widianto; Irfan D. Prijambada
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.173

Abstract

The aims of this study were to investigate the effects of the mixture of tapioca waste, rice bran, starch (BOC), and humic substance (BH) on Aspergillus niger growth and its phosphate solubilizing capability. The experiment was conducted in Erlenmeyer flask containing 200 ml Pikovskaya liquid media and incubated on shaker with 100 rotaries per minute for nine days.  The Christmas Island phosphate rock was used as phosphate source.  Aspergillus niger YD 17 was taken from the Laboratory of Microbiology, Faculty of Agriculture, Gadjah Mada University. The experiment was arranged using factorial completely randomized design with three-replications (3x4x3). The level of BOC (i.e. 0, 2, and 4 g.l-1 media)   and BH   (i.e. 0, 1, 2, and 3 ml.l-1) were applied as experimental factors. Dry weight of mycelia and the soluble phosphate in media were parameters of Aspergillus niger growth. The results showed that BOC and BH increased the Aspergillus niger growth and its phosphate solubilizing capability (P£0.05). Four-gram of BOC and 3 ml of BH per litter of media were the best level for Aspergillus niger growth, while 4 g of BOC and 2 ml of BH was the best level for phosphorus solubilization capability.
Co-Authors Bostang Radjagukguk Donny Widianto Erni Martani Yanisworo W. Ratih Yudi Sastro